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30,870 results

Showing: 3581 - 3600 of 30870 results

  2. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196055-plasmid-map-sequence-id-385866

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  3. pB80-hKif5b(1-560)rigor-2xmNeongreen

    Plasmid
    #174649
    Purpose
    Live-cell marker for stable microtubules
    Depositor
    Lukas Kapitein
    Publication
    Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
    Tags
    mNeongreen-mNeongreen
    Expression
    Mammalian
    Mutation
    rigor mutation G234A
    Promoter
    Chicken beta-actin
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174649-plasmid-map-sequence-id-345295

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  4. pDAC446

    Plasmid
    #195239
    Purpose
    Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Publication
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Expression
    Mammalian
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195239-plasmid-map-sequence-id-382947

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  5. G13-CASE

    Plasmid
    #168127
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F125/D126 within GNA13
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168127-plasmid-map-sequence-id-332872

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  9. pDONR221_SLC3A2

    Plasmid
    #132213
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC3A2 (SLC3A2 Human)
    Expression
    Mammalian
    Available since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    132213-plasmid-map-sequence-id-261916

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  10. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid
    #127354
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboID (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, V5, and mVenus
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127354-plasmid-map-sequence-id-246338

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  11. pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

    Plasmid
    #129722
    Purpose
    AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin
    Depositor
    Elina Ikonen
    Publication
    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Seipin (BSCL2 Human)
    Use
    CRISPR and TALEN; Human safe harbor locus (a avs1…
    Tags
    miniIAA7-3XFlag
    Expression
    Mammalian
    Mutation
    silent mutations
    Promoter
    EF1a
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    129722-plasmid-map-sequence-id-254817

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  12. pH2B-miRFP670nano

    Plasmid
    #127438
    Purpose
    Histone 2B labeling with near-infrared fluorescent protein miRFP670nano
    Depositor
    Vladislav Verkhusha
    Publication
    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-miRFP670nano (H2BC11 Synthetic, Human)
    Tags
    miRFP670nano
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127438-plasmid-map-sequence-id-250415

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  14. pcDNA3-ExRai-CKAR

    Plasmid
    #118409
    Purpose
    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-CKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    118409-plasmid-map-sequence-id-243085

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  19. pX-evoCas9

    Plasmid
    #107550
    Purpose
    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells
    Depositor
    Anna Cereseto
    Publication
    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    M495V/Y515N/K526E/R661Q
    Promoter
    CBh
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107550-plasmid-map-sequence-id-206015

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Showing: 3581 - 3600 of 30870 results