27,007 results
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Plasmid#98285Purposemammalian expression of CTLA4-mEos2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTLA4 (CTLA4 Human)
TagsmEos2ExpressionMammalianPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)
Plasmid#69138PurposeFor the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
TagsnoneExpressionMammalianAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNTI194
Plasmid#84266PurposeExpresses Avi-tagged RPL1 and humanized birADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsAvi tag, Flag, Gly/Ser linker, and TEV siteExpressionMammalianMutationR33A, A34E (*see below)PromoterCMV and P2A peptideAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPaint-2A-TurboGFP-PEST
Plasmid#67180PurposeCRISPaint universal donor for gene expression quantification using destabilized TurboGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT2A-TurboGFP-PEST
Available sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1
Plasmid#81047Purposemammalian expression of Zdk1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZdk1
Tags6x His tag, FLAG, and mVenusExpressionBacterial, Insect, and Mamm…Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGCaMP6m-N3-TPC2
Plasmid#80147PurposeGCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real timeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTPCN2 (TPCN2 Human)
TagsGCaMP6mExpressionMammalianPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG sHRPb-NLG
Plasmid#73144PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsHRPb-NLG (Nlgn1 Synthetic, Mouse)
TagsV5 tagExpressionMammalianPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic FAK biosensor
Plasmid#78300PurposeFRET biosensor. Visualization of cytosolic FAK activity in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCytosolic FAK biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lyn FAK biosensor
Plasmid#78299PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn Fak biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PXL
Plasmid#75349PurposePX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRExpressionMammalianPromoterU6Available sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKanCMV-mClover3-18aa-actin
Plasmid#74259PurposeMammalian expression of N-terminal fusion of mClover3 to actinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertActin
TagsmClover3ExpressionMammalianPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmGFP10C-Tau
Plasmid#71433PurposeSplit GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTau (0N4R) (MAPT Human)
TagsGFP (1-212)ExpressionMammalianAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cx43-msfGFP
Plasmid#69007PurposeExpresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGja1 (Gja1 Rat)
Tagsmonomerized superfolder GFPExpressionMammalianPromotercmvAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OPRK1-Tango
Plasmid#66462PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOPRK1 (OPRK1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MRGPRX2-Tango
Plasmid#66440PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMRGPRX2 (MRGPRX2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AGTR1-Tango
Plasmid#66222PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAGTR1 (AGTR1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mRFP-FKBP12-5ptpase domain
Plasmid#67516PurposeRecruitable human type IV 5-phosphatase domain (214-644)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsmRFPMutationINPP5E has C641A to destroy the C-terminal CAAX d…Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_ST-SBP-EGFP
Plasmid#65264Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p189
Plasmid#65207PurposeMutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
ExpressionBacterial and MammalianMutationPremature stop codon added to pGEM5Z(+)-EGFP (TGG…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP rbFOX2
Plasmid#63086PurposeMammalian expression of EGFP tagged FOX2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRBFOX2 (RBFOX2 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by