Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,870 results

Showing: 4641 - 4660 of 30870 results

  1. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

    Plasmid
    #172910
    Purpose
    Mammalian expression of voltage sensor; soma localization targeting sequence
    Depositor
    GENIE Project
    Publication
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Voltron2-ST
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    CAG
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172910-plasmid-map-sequence-id-341196

    Enlarge

  2. pcDNA3-pmEKAR4

    Plasmid
    #174440
    Purpose
    Plasma membrane-targeted EKAR4.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pmEKAR4
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174440-plasmid-map-sequence-id-344974

    Enlarge

  3. UBC plasmid

    Plasmid
    #169746
    Purpose
    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.
    Depositor
    Francois St-Pierre
    Publication
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    UBC
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169746-plasmid-map-sequence-id-334556

    Enlarge

  4. pC1-CMV-mito-DAAO

    Plasmid
    #141132
    Purpose
    Expresses DAAO with mitochondrial localization signal in mammalian cells
    Depositor
    Vsevolod Belousov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mito-DAAO
    Tags
    2 mitochondrial localization signals and 7xHis
    Expression
    Mammalian
    Mutation
    deleted DAAO amino acids 367-368
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    141132-plasmid-map-sequence-id-323598

    Enlarge

  8. CGBE1-NG (pBM1246)

    Plasmid
    #140256
    Purpose
    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eUNG-BE4max(R33A)_NG-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140256-plasmid-map-sequence-id-291778

    Enlarge

  9. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140982-plasmid-map-sequence-id-300744

    Enlarge

  10. pcDNA4/TO/Strep-HA-ZAK alpha

    Plasmid
    #141193
    Purpose
    Expresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line
    Depositor
    Simon Bekker-Jensen
    Publication
    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAP3K20 (MAP3K20 Human)
    Tags
    Strep, HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141193-plasmid-map-sequence-id-299542

    Enlarge

  13. Fibronectin-human-EDA in pMAX

    Plasmid
    #120402
    Purpose
    enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence
    Depositor
    Inaam Nakchbandi
    Publication
    Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EDA-fibronectin (FN1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120402-plasmid-map-sequence-id-251099

    Enlarge

  19. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid
    #109121
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    109121-plasmid-map-sequence-id-209767

    Enlarge

  20. dxCas(3.6)-VPR

    Plasmid
    #108388
    Purpose
    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dxCas(3.6)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108388-plasmid-map-sequence-id-207184

    Enlarge

Showing: 4641 - 4660 of 30870 results