Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,870 results

Showing: 4681 - 4700 of 30870 results

  2. pcDNA3.1-KozATG-dL5-2XG4S-mCer3

    Plasmid
    #73207
    Purpose
    Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KozATG-dL5-2XG4S-mCer3
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73207-plasmid-map-sequence-id-139724

    Enlarge

  3. pHBV-X/EnhI-Luc

    Plasmid
    #71418
    Purpose
    To see HBV X promoter(overlaped EnhanceI) activity for luciferase assay
    Depositor
    Wang-Shick Ryu
    Publication
    Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HBV X promoter and enhancer I driving Luciferase expression
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    HBV X
    Available since
    Dec. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71418-plasmid-map-sequence-id-128516

    Enlarge

  4. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Publication
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-dCas9VP192-T2A-EGFP
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69534-plasmid-map-sequence-id-122831

    Enlarge

  5. HTR1A-Tango

    Plasmid
    #66404
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTR1A (HTR1A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66404-plasmid-map-sequence-id-417855

    Enlarge

  6. LPAR1-Tango

    Plasmid
    #66418
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LPAR1 (LPAR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66418-plasmid-map-sequence-id-244390

    Enlarge

  7. FFA1-Tango

    Plasmid
    #66280
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FFA1 (FFAR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  8. CCR2-Tango

    Plasmid
    #66239
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCR2 (CCR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  9. CHRM1-Tango

    Plasmid
    #66248
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHRM1 (CHRM1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  10. BLBP-GFP

    Plasmid
    #63174
    Purpose
    Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter
    Depositor
    Bart De Strooper
    Publication
    Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BLBP (Fabp7 Mouse)
    Expression
    Mammalian
    Promoter
    1700bp upstream of BLBP gene
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63174-plasmid-map-sequence-id-117985

    Enlarge

  12. GFP-TRIM24

    Plasmid
    #65396
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRIM24 (TRIM24 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. EGFP-Galectin1-9

    Plasmid
    #56434
    Purpose
    Localization: Glycoproteins, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Galectin1 (LGALS1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56434-plasmid-map-sequence-id-522615

    Enlarge

  17. SP6-VEE-OKS-iM

    Plasmid
    #58973
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Publication
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58973-plasmid-map-sequence-id-91416

    Enlarge

  18. pCMV-SNAP-CRY2-VHH(GFP)

    Plasmid
    #58370
    Purpose
    For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP
    Depositor
    Won Do Heo
    Publication
    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRY2[1-498] (CRY2 Mustard Weed)
    Tags
    SNAP and vhhGFP4
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58370-plasmid-map-sequence-id-90205

    Enlarge

  19. mEmerald-WASP1-N-14

    Plasmid
    #54315
    Purpose
    Localization: Cytoskeleton, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WASP1 (WASF1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54315-plasmid-map-sequence-id-298515

    Enlarge

  20. PML-3/pSG5

    Plasmid
    #50939
    Purpose
    expression of PML with Sumo sites destroyed
    Depositor
    Pier Pandolfi
    Publication
    Zhong et al Blood. 2000 May 1;95(9):2748-52.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PML-3 (PML Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K65R, K160R, K490R (Sumo modification sites)
    Promoter
    SV40
    Available since
    Jan. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 4681 - 4700 of 30870 results