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27,006 results

Showing: 4681 - 4700 of 27006 results

  4. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    183411-plasmid-map-sequence-id-361257

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  7. pC1-CMV-mito-DAAO

    Plasmid
    #141132
    Purpose
    Expresses DAAO with mitochondrial localization signal in mammalian cells
    Depositor
    Vsevolod Belousov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mito-DAAO
    Tags
    2 mitochondrial localization signals and 7xHis
    Expression
    Mammalian
    Mutation
    deleted DAAO amino acids 367-368
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    141132-plasmid-map-sequence-id-323598

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  13. CGBE1-NG (pBM1246)

    Plasmid
    #140256
    Purpose
    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eUNG-BE4max(R33A)_NG-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140256-plasmid-map-sequence-id-291778

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  14. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    140982-plasmid-map-sequence-id-300744

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  15. pcDNA4/TO/Strep-HA-ZAK alpha

    Plasmid
    #141193
    Purpose
    Expresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line
    Depositor
    Simon Bekker-Jensen
    Publication
    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAP3K20 (MAP3K20 Human)
    Tags
    Strep, HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    141193-plasmid-map-sequence-id-299542

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  16. pcDNA3-sACE2(WT)

    Plasmid
    #145147
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    145147-plasmid-map-sequence-id-292583

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  19. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid
    #129668
    Purpose
    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus
    Depositor
    Michael Guertin
    Publication
    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OsARF16-PB1 domain
    Tags
    GFP
    Expression
    Mammalian
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    129668-plasmid-map-sequence-id-254335

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Showing: 4681 - 4700 of 27006 results