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30,870 results

Showing: 5021 - 5040 of 30870 results

  2. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  4. RANLS

    Plasmid
    #50843
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50843-plasmid-map-sequence-id-76292

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  7. NOVA1 eGFP

    Plasmid
    #61275
    Purpose
    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1
    Depositor
    Nicolas Charlet-Berguerand
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NOVA1 (NOVA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61275-plasmid-map-sequence-id-99586

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  10. mCherry-WASH1-C-18

    Plasmid
    #55162
    Purpose
    Localization: Cytoskeleton, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WASH1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55162-plasmid-map-sequence-id-492813

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  13. pMH3

    Plasmid
    #52528
    Purpose
    serves as PCR-template to generate HR donors for C-terminal GFP-tag
    Depositor
    Klaus Foerstemann
    Publication
    Bottcher et al Nucleic Acids Res. 2014 Apr 19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP and Blasticigin resistance cassette
    Use
    Blunt cloning kit
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52528-plasmid-map-sequence-id-81488

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  15. p2attNG-H11-long

    Plasmid
    #51545
    Purpose
    p2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.
    Depositor
    Michele Calos
    Publication
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP4m
    Use
    Human targeting
    Expression
    Mammalian
    Available since
    March 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51545-plasmid-map-sequence-id-78773

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  16. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid
    #49060
    Purpose
    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Publication
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP (chloride-sensitive)
    Expression
    Mammalian
    Mutation
    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…
    Promoter
    CMV
    Available since
    Nov. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    49060-plasmid-map-sequence-id-72178

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  18. pAC153-dual-dCas9VP96-sgExpression

    Plasmid
    #48239
    Purpose
    Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    HA-Tag, HA-tag, and VP96
    Expression
    Mammalian
    Mutation
    D10A H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48239-plasmid-map-sequence-id-69546

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Showing: 5021 - 5040 of 30870 results