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30,881 results

Showing: 8141 - 8160 of 30881 results

  5. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid
    #208336
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
    Depositor
    Pranam Chatterjee
    Publication
    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Sc++ (1-1119), SpRY (1111-1368), D10A
    Promoter
    CAG
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208336-plasmid-map-sequence-id-439176

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  6. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid
    #201254
    Purpose
    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells
    Depositor
    Alexander Kleger
    Publication
    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAS (KRAS Human)
    Use
    Piggybac transposon
    Tags
    HA-Tag
    Expression
    Mammalian
    Mutation
    changed Gly (G) 12 to Asp (D)
    Promoter
    TRE
    Available since
    Sept. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    201254-plasmid-map-sequence-id-408509

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  11. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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  13. pCMV6-AN-HA_WT POLH

    Plasmid
    #201671
    Purpose
    Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells
    Depositor
    Roger Woodgate
    Publication
    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Polymerase eta (POLH Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Coding sequence has been optimised for expression…
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    201671-plasmid-map-sequence-id-394899

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  15. pcDNA3.1-FGFR2c-V5/HIS

    Plasmid
    #201107
    Purpose
    expression of human FGFR2 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    201107-plasmid-map-sequence-id-394499

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  16. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enOsCas12f1
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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  18. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid
    #197031
    Purpose
    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized denOsCas12f1-VPR
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197031-plasmid-map-sequence-id-388973

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  19. pCDNA3.1_WiChR_TS_mScarlet_ER

    Plasmid
    #195190
    Purpose
    High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells
    Depositor
    Peter Hegemann, Johannes Vierock
    Publication
    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Wobblia lunata inhibitory Channelrhodopsin
    Tags
    ER-export sequence, Kir2.1 trafficking motif, and…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    195190-plasmid-map-sequence-id-390435

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  20. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid
    #189755
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Publication
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    189755-plasmid-map-sequence-id-385774

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Showing: 8141 - 8160 of 30881 results