27,462 results
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Plasmid#79005PurposeExpresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPrkaa2 (Prkaa2 Mouse)
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4
Plasmid#78349PurposeExpresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)
UseSynthetic BiologyTagsrTetRExpressionMammalianPromoterpEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HSV-DYN-hM4Di
Plasmid#53327PurposeDynorphin promoter driving hM4DiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthM4Di (Pdyn Rat)
UseHsvTags3xHA tagExpressionMammalianPromoterRat dynorphin promoter ( -1858 to +135)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL
Plasmid#73209PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertkappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
TagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pE478G-EGFP
Plasmid#68848Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertoptineurin (OPTN Human)
TagsEGFPExpressionMammalianMutationE478G, associated with ALSPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H2B-GFP-AsLOV2-NES27 (pDN158)
Plasmid#72659PurposeConstitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear exportDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-GFP-AsLOV2-NES27
ExpressionMammalianPromoterCMVAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmVenus-C2-MmMKL2
Plasmid#67894PurposeExpresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmVenus-MKL2
ExpressionMammalianPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMM733
Plasmid#68898PurposeIPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9
LacI
sgRNA
NanoLuc
Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-alpha-E-Catenin 3xFP4 (895)
Plasmid#67936PurposeMammalian expression of alpha E cateninDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha E catenin (Ctnna1 Mouse)
Tags3xFP4 and EGFPExpressionMammalianMutationN-terminal domainPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cx26-msfGFP
Plasmid#69016PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCx26 (Gjb2 Rat)
TagsV206K monomerized super folder GFPExpressionMammalianPromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ155
Plasmid#69306PurposeGateway entry vector with AteCas9D10ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAteCas9D10A (Arabidopsis codon optimized)
UseCRISPRMutationD10A mutation; NLS fusionAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
PTGER3-Tango
Plasmid#66485PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEP3-I (PTGER3 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SCTR-Tango
Plasmid#66501PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSCTR (SCTR Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SUCNR1-Tango
Plasmid#66507PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSUCNR1 (SUCNR1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
P2RY1-Tango
Plasmid#66468PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP2RY1 (P2RY1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MC3R-Tango
Plasmid#66429PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMC3R (MC3R Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
HTR4-Tango
Plasmid#66412PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTR4 (HTR4 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPR88-Tango
Plasmid#66380PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR88 (GPR88 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GRM8-Tango
Plasmid#66393PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGRM8 (GRM8 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GPR35-Tango
Plasmid#66354PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR35 (GPR35 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only