30,881 results
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Plasmid#129087PurposeExpression of luxC in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertco luxC
ExpressionMammalianPromoterCMVAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
co luxD pcDNA3.1(+)
Plasmid#129088PurposeExpression of luxD in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertco luxD
ExpressionMammalianPromoterCMVAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
co frp pcDNA3.1(+)
Plasmid#129090PurposeExpression of frp in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertco frp
ExpressionMammalianPromoterCMVAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Lifeact_Halo_T2A_EGFP
Plasmid#135445PurposeMamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifact-HaloTag7 and EGFP
TagsLifactExpressionMammalianPromoterCMV/TetOAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 1c
Plasmid#134832PurposeExpression of rat Myo 1c (Myr 2) in mammalian cells. N-terminal GFP-tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyo 1c (rat) with 3' UTR (Myo1c Rat)
TagsEGFPExpressionMammalianPromoterCMV IEAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA
Plasmid#134924PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
ExpressionMammalianPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC38A1
Plasmid#132095PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSLC38A1 (SLC38A1 Human)
ExpressionMammalianAvailable sinceDec. 2, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
PX458-3xHA-FnCas9
Plasmid#130969Purpose3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFnCas9
UseCRISPRTags3xHA-NLS and NLS-T2A-EGFPExpressionMammalianPromoterCbhAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR221_SLC7A1
Plasmid#131925PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSLC7A1 (SLC7A1 Human)
ExpressionMammalianAvailable sinceOct. 16, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDONR221_MFSD2A
Plasmid#131893PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMFSD2A (MFSD2A Human)
ExpressionMammalianAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA3.1(+)-OTI-TCRA_p2_TCRB
Plasmid#131033PurposeExpresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptideDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sox2-t2A-mCherry
Plasmid#127538PurposeExpresses human SOX2 and mCherry via t2A linker.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSOX2 (SOX2 Human)
ExpressionMammalianPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eGFP L202 gRNA
Plasmid#119132PurposegRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP L202 gRNA
UseCRISPRMutationNonePromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUF1-dCas9-GCN5
Plasmid#122509PurposeCombining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-GCN5
UseCRISPRTags3*FLAGMutationdCas9(D10A,H840A)PromoterPfHsp86Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-RAB-EKARev
Plasmid#118477PurposeddRFP-based single-color ERK biosensor targeted to the plasma membrane.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn-RAB-EKARev
TagsN-terminal targeting sequence from Lyn-kinaseExpressionMammalianPromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B G120A
Plasmid#123115PurposeExpresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsEGFPExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Orange_CaMBI_110
Plasmid#124094Purposeorange calcium-modulated bioluminescent indicator, 110nMDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOrange CaMBI 110
ExpressionMammalianAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119946PurposeExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla luciferase
Tags5'TOP sequence, 24xGCN4 repeats (SunTag), 24…ExpressionBacterial and MammalianPromoterTetCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-eGFP-CAAX-SV40
Plasmid#117815PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyTagsCAAXAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-SV40
Plasmid#117811PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyTagsNLSAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by