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30,881 results

Showing: 10241 - 10260 of 30881 results

  4. pDD428

    Plasmid
    #202081
    Purpose
    Cas9/sgRNA plasmid targeting the C-terminus of Myh9
    Depositor
    Daniel Dickinson
    Publication
    Shi et al Development. 2023 May 15;150(10):dev2013
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
    Use
    CRISPR
    Promoter
    EF1a
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202081-plasmid-map-sequence-id-399529

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  6. pcDNA3.1-FGFR4-V5/HIS

    Plasmid
    #201109
    Purpose
    expression of human FGFR4 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    201109-plasmid-map-sequence-id-394498

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  8. Membrane mNeonGreen

    Plasmid
    #198057
    Purpose
    Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LCK membrane mNeonGreen (LCK A.victoria, Human)
    Use
    Synthetic Biology; Sea urchin, mammal, zebrafish
    Tags
    mNeonGreen
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198057-plasmid-map-sequence-id-389150

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  9. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196078-plasmid-map-sequence-id-388989

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  14. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Elvir Becirovic
    Publication
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    TRE and U6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    166693-plasmid-map-sequence-id-331043

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  15. LYSO-LRRK2

    Plasmid
    #193582
    Purpose
    Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus
    Depositor
    Mark Cookson
    Publication
    Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Leucine-rich repeat kinase 2 (LRRK2 Human)
    Tags
    LAMTOR1(1-39aa) and 3xFLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    193582-plasmid-map-sequence-id-382173

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  16. pAK_DR30_CasRx_GFP

    Plasmid
    #134843
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    134843-plasmid-map-sequence-id-266705

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  17. CABE-T3.155

    Plasmid
    #193270
    Purpose
    Expression of CABE-T3.155 base editor in mammalian cells, CMV promoter
    Depositor
    Nicole Gaudelli
    Publication
    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CABE-T3.155
    Expression
    Mammalian
    Mutation
    D10A (Cas9), TadA mutations within
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193270-plasmid-map-sequence-id-379827

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  18. CBE-T1.14

    Plasmid
    #193277
    Purpose
    Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter
    Depositor
    Nicole Gaudelli
    Publication
    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CBE-T1.14
    Expression
    Mammalian
    Mutation
    D10A (Cas9), TadA mutations within
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193277-plasmid-map-sequence-id-379839

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Showing: 10241 - 10260 of 30881 results