31,663 results
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Plasmid#218368PurposeEmpty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sitesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only
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pHH0103_PCMT1_PCMT
Plasmid#109932PurposeProtein expression and purification of PCMT1_PCMTDepositorInsertPCMT1_PCMT
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2_SEPT6
Plasmid#174497Purposebacterial co-expression of human SEPT2 and of human SEPT6DepositorAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
ExpressionBacterialPromoterglnS and pBADAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
ExpressionBacterialAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
elavl3:hArr-TEV;he1.1:YFP
Plasmid#218855PurposeD. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.DepositorInserthArr
Tagshuman Arrestin-Tobbaco Etch Virus protease fusion…ExpressionBacterialAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPerimCh
Plasmid#227013PurposeExpresses periplasmic mCherryDepositorInsertPelB-mCherry, arabinose inducible cytosolic GFP
ExpressionBacterialPromoterrpsM and pBADAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWUR 1+2 tetO mut89
Plasmid#80102PurposeEncodes a set of wild-type Cas1+2 and a mutant set of Cas1+2DepositorExpressionBacterialMutationQ24H, P202Q, G241D, E276D, L297QAvailable SinceDec. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
NW9
Plasmid#133442PurposeCovalently circularized nanodisc NW9 for studying membrane proteins and viral entry.DepositorInsertNW9
ExpressionBacterialAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-SCR-sgRNA-A
Plasmid#177811PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAJM.657
Plasmid#108525PurposeCymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.DepositorInsertCymRAM + PCymRC-YFP
ExpressionBacterialMutationCymR: S110G A171VAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV643
Plasmid#226182PurposeExpresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.DepositorInsertuidA marker flanked with targeting regions to KU70 gene in K.phaffii
ExpressionBacterialPromoterTEF1pAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvLEAM
Plasmid#90033PurposeTo express His-tagged RvLEAM in bacteriaDepositorInsertRvLEAM
TagsHisExpressionBacterialPromoterT7Available SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_rfp
Plasmid#194155PurposeRed Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
ExpressionBacterialPromoterPoxyR, PoxySAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-Nanog
Plasmid#16351DepositorInsertHomo sapiens Nanog homeobox (NANOG Human)
ExpressionBacterialAvailable SinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T MDM2 WT
Plasmid#16237DepositorAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184741Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-A20 (OTU, aa 1-366)
Plasmid#61584PurposeExpresses human A20 (OTU domain) in E. coli.DepositorInsertA20 (TNFAIP3 Human)
TagsGST-3CExpressionBacterialMutationDeleted aa 367-790.PromotertacAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialMutationMissing first 408bpAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM253 (miniCTX1-rhaSR-PrhaBAD)
Plasmid#110571PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
ExpressionBacterialPromoterrhaSR-PrhaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only