30,738 results

1. pGEM4Z-EGFP

   Plasmid

   #183475

   Purpose

   This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

   Depositor

   Chantal Pichon

   Article

   Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

   Insert

   EGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-15b-TEV-C9R

   Plasmid

   #118754

   Purpose

   TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

   Depositor

   Yutaka Kuroda

   Article

   Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

   Insert

   Tobacco Etch Virus protease (NEWENTRY Synthetic)
   

   Tags

   C9R and His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLRT

   Plasmid

   #78234

   Purpose

   Expresses Murine Leukemia Reverse Transcriptase (MLRT)

   Depositor

   Christopher Voigt

   Article

   Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

   Insert

   Murine leukemia reverse transcriptase
   

   Expression

   Bacterial

   Promoter

   J23102

   Available Since

   Aug. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAIP4_2PPN

   Plasmid

   #234190

   Purpose

   Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2PPN (FKBP1A Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pQE30-roGFP1-iR

   Plasmid

   #83314

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

   Insert

   roGFP1-iR
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

   Promoter

   T5

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pBK- Ma PylRS WT

   Plasmid

   #197573

   Purpose

   Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Insert

   M. alvus PylRS WT
   

   Expression

   Bacterial

   Promoter

   GlnS (constitutive)

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-smURFP-RBS-HO-1

   Plasmid

   #80341

   Purpose

   Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   HO-1
   

   Tags

   6 Histidine Tag

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRS-XylS-dCas9

   Plasmid

   #220196

   Purpose

   mini-Tn7 dCas9 expression vector - XylS/pM promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   XylS/pM

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSP3535

   Plasmid

   #46886

   Purpose

   Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

   Depositor

   Gary Dunny

   Article

   Bryan et al Plasmid. 2000 Sep;44(2):183-90.

   Type

   Empty backbone

   Use

   Nisin-inducible expression; gram-positive bacteri…

   Expression

   Bacterial

   Promoter

   PnisA

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCB591

    Plasmid

    #141096

    Purpose

    E. coli-Lactobacilli shuttle vector for recombineering template cloning

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-Cdc42

    Plasmid

    #12201

    Depositor

    Jonathan Chernoff

    Article

    Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET19-TdTwt

    Plasmid

    #207466

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTwt (Dntt Mouse)
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETMCSI-T4P_Y320A

    Plasmid

    #122522

    Purpose

    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

    Depositor

    Gottfried Otting

    Article

    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

    Insert

    gp43 - DNA polymerase (43 Escherichia virus T4)
    

    Expression

    Bacterial

    Mutation

    tyrosine 320 changed to alanine

    Promoter

    T7 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKM2

    Plasmid

    #25538

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PKM2 (PKM Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pNP568

    Plasmid

    #222725

    Purpose

    pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Tags

    sfGFP

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDB302

    Plasmid

    #113673

    Purpose

    expresses Cth SUMO protease in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Cth SUMO protease
    

    Tags

    10-His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-934

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-LIC

    Plasmid

    #26098

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    Three consecutive Taq promoters, 2 active

    Expression

    Bacterial

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pFC902

    Plasmid

    #106904

    Purpose

    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108

    Inserts

    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PEmax_2star_pet21A

    Plasmid

    #227675

    Purpose

    Expression plasmid for PEMAX** protein in bacteria.

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    PEmax_2_star
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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