32,126 results
-
Plasmid#119882PurposeConstitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpConstitutive_GFP+
ExpressionBacterialAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG012 (DRT type 4)
Plasmid#157890PurposeExpresses the DRT type 4 defense systemDepositorInsertDRT type 4
ExpressionBacterialAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG013 (DRT type 5)
Plasmid#157891PurposeExpresses the DRT type 5 defense systemDepositorInsertDRT type 5
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204816PurposeBacterial expresison of Enterovirus A71 3C proteaseDepositorInsertEV A71 3C protease
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTlpA39-Wasabi
Plasmid#86116PurposemWasabi reporter gene gated by TlpA39DepositorInsertsTlpA39
mWasabi
Tags6xHisExpressionBacterialMutationD341A, D135V, A217V, L236FPromoterpTlpA and pTlpA, LacIAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutS
Plasmid#86605PurposeE. coli MutS expression plasmidDepositorInsertE. coli MutS (D835R,R840E)
Tagshis-tag and ald-tagExpressionBacterialMutationD835R, R840EPromoterT7Available SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PFO
Plasmid#92109PurposeRecombinant expression in E.ColiDepositorInsertPFO
Tags6xHisExpressionBacterialMutationDelta27aaAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-beta-arrestin2
Plasmid#36919DepositorAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTCas9_scr
Plasmid#207566PurposeThermoCas9-mediated cleavage of genomic DNA in E. coliDepositorInsertPlac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-Pho8∆60-HphMX6
Plasmid#240973PurposeTo endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection markerDepositorInsertPHO8∆60 (PHO8 Budding Yeast)
ExpressionBacterialAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_FL Gcn4
Plasmid#231855PurposeBacterial expression of N-terminally 6His tagged FL Gcn4DepositorInsertGcn4
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHelper-CRISPRi-sgRNA
Plasmid#221135PurposeHelper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with dCas9 handle
UseCRISPRExpressionBacterialPromoterJ23119(SpeI)Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-CRISPRi
Plasmid#221136PurposeCoxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.DepositorInsert3xF-dCas9
UseCRISPRTags3xFLAGExpressionBacterialPromotercbu1169 promoterAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-L1
Plasmid#205033PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-L1
TagsGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CD11b promoter in pGEM3zf(-)
Plasmid#26168DepositorInsertCD11b promoter (ITGAM Human)
ExpressionBacterialAvailable SinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEwt2/Imwt2
Plasmid#107127Purposeplasmid for expression of WT colicin endonuclease and immunity pair 2DepositorInsertcolEwt2/Imwt2
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-CLIP-KanMX6
Plasmid#87025PurposeTag S. pombe genes in C terminal with CLIPDepositorInsertCLIP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Himar-dCas9
Plasmid#137079PurposeHimar-dCas9 gene with C-terminal 6x-His tag for protein purificationDepositorInsertHimar-dCas9
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTdTomato-L5
Plasmid#140994PurposetdTomato, StrepR, L5int, attP to create RFP mycobacteriaDepositorInserttdTomato
ExpressionBacterialPromoterhsp60Available SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
TXNRD1
Plasmid#38863PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP1a
Plasmid#205304PurposeExpression of WHaloCaMP1a in E. coliDepositorInsertWHaloCaMP1a
ExpressionBacterialAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-IF1
Plasmid#124123PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1TFAKRS
Plasmid#177311PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Tre-C04
Plasmid#79754PurposeSingle fluorescent protein biosensor for trehaloseDepositorInsertTre-C04
Tags6xHisExpressionBacterialPromoterpTrcAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRM049 His-TEV-Cas9 triNLS 2C
Plasmid#196245PurposeExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGNW2
Plasmid#122086Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP22x-1A(sfGFP)
Plasmid#126969PurposeLevel 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA
Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Q-IL6-9
Plasmid#166552PurposescFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptideDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only