29,712 results

1. pJMP8823

   Plasmid

   #220901

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx-RhoA FLARE.sc Biosensor WT

   Plasmid

   #12150

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor (RHOA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDB302

   Plasmid

   #113673

   Purpose

   expresses Cth SUMO protease in Escherichia coli

   Depositor

   Christopher Bahl

   Article

   Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

   Insert

   Cth SUMO protease
   

   Use

   Tags

   10-His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-934

   Promoter

   T7

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCV019-catP

   Plasmid

   #232825

   Purpose

   Random transposon mutagenesis vector for Bacteroidales species - chloramphenicol

   Depositor

   Athanasios Typas

   Insert

   catP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-FUS_FL_6E

   Plasmid

   #98655

   Purpose

   Expresses MBP-tagged full length phosphomimetic FUS

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   6 S/T to E

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLITMUS*-PBBa_J23106-geneIII

   Plasmid

   #134352

   Purpose

   Phagemid

   Depositor

   Mark Isalan

   Article

   Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

   Insert

   Gene III
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC28-DENV3-NS5FL

   Plasmid

   #86013

   Purpose

   Expression of dengue virus 3 NS5 protein

   Depositor

   Dahai Luo

   Article

   Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

   Insert

   DENV3-NS5FL
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   amino acid residues 6 to 895 of DENV3 NS5

   Promoter

   T7

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRST11B-AS3_4

   Plasmid

   #102789

   Purpose

   Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Inserts

   tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
   suppressor tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pH6HTC_ABCE1-Halo

   Plasmid

   #175335

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   ABCE1 (ABCE1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

    Plasmid

    #174515

    Purpose

    Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Inserts

    Mm PylS
    MmtRNA-Pyl-opt(UGA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ASL from the serT gene (UGA anticodon) and recoded

    Promoter

    GlnS and lpp

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTCF236 HLA-B*40:01

    Plasmid

    #180463

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*40:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pet28a-His6-Keap1

    Plasmid

    #62454

    Purpose

    His6 tagged Keap1 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_PLK1_Pkinase

    Plasmid

    #109933

    Purpose

    Protein expression and purification of PLK1_Pkinase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PLK1_Pkinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA6.MCV.LT339.V5.4

    Plasmid

    #28193

    Depositor

    Patrick Moore

    Article

    Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

    Insert

    MCPyV Truncated Tumor Large T antigen (399 tumor strain)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINA

    Plasmid

    #41141

    Depositor

    Ray Owens

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET41-MBP-t-A3A-t-His

    Plasmid

    #109231

    Purpose

    Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

    Depositor

    Rahul Kohli

    Article

    Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

    Insert

    human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mu-IFNg-pCIneo

    Plasmid

    #163517

    Purpose

    Expresses murine IFN gamma in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine-IFN gamma (Ifng Mouse)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF-CjPglB

    Plasmid

    #199305

    Purpose

    pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    CjPglB with flag tag
    

    Use

    Tags

    Flag tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. KLHL12

    Plasmid

    #38908

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL12 (KLHL12 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET-His-Cre

    Plasmid

    #123128

    Purpose

    Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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