30,738 results

1. pAJM.336

   Plasmid

   #108528

   Purpose

   LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   LacIAM + PTac-YFP
   

   Expression

   Bacterial

   Mutation

   LacI Q18M A47V F161Y

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FusionRed-pBAD

   Plasmid

   #54677

   Purpose

   Localization: Protein Expression Vector , Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Type

   Empty backbone

   Tags

   6xHis and FusionRed

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pKSJ376

   Plasmid

   #103065

   Purpose

   Expresses colicin E1 with R domain deletion and ImmE1

   Depositor

   Alan Finkelstein, Karen Jakes

   Article

   Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

   Inserts

   colicin E1 immunity protein (pColE1_26, imm E. coli)
   cea/colicin E1
   

   Expression

   Bacterial

   Mutation

   R (receptor-binding domain) deletion in colicin E…

   Promoter

   T7 lac (from pET) and T7 lac (from pET52-b)

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET11phiC31poly(A)

   Plasmid

   #18942

   Depositor

   Michele Calos

   Article

   Groth et al Genetics. 2004 Apr . 166(4):1775-82.

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-At-TRL

   Plasmid

   #32242

   Depositor

   Stan Fields, Jay Hesselberth

   Article

   Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

   Insert

   A. thaliana tRNA ligase (RNL Mustard Weed)
   

   Tags

   6xHistidine

   Expression

   Bacterial

   Promoter

   T7 RNA Polmyerase

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExp-sfGFP

   Plasmid

   #112573

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis, Strep II, and sfGFP

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET264-pUC 24xMS2V6 Loxp KANr Loxp

   Plasmid

   #104393

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-16

   Plasmid

   #172746

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-16
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

    Plasmid

    #187356

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)_beta2M (MHC Class I light)

    Plasmid

    #187357

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    beta2M (MHC Class I light)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3

    Plasmid

    #204808

    Purpose

    Bacterial co-expression of Dengue2 NS2B and NS3 proteins

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Dengue2 NS2B (POLY Dengue2)
    

    Expression

    Bacterial

    Available Since

    Dec. 18, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pSUC01

    Plasmid

    #177204

    Purpose

    chromogenic reporter for gene knockout in Streptomyces

    Depositor

    Wenjun Guan

    Article

    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

    Insert

    SaindC
    

    Use

    Synthetic Biology

    Tags

    NONE

    Expression

    Bacterial

    Mutation

    NONE

    Promoter

    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. UbcH5c S22R pET28a

    Plasmid

    #12644

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubch5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Expression

    Bacterial

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

15. JpExpress404 PTEN-LONG

    Plasmid

    #49417

    Purpose

    Bacterial Expression Vector

    Depositor

    Ramon Parsons

    Article

    Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

    Insert

    PTEN-Long (PTEN )
    

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-SerRS-His

    Plasmid

    #124118

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Seryl-tRNA ligase (serS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHimar6

    Plasmid

    #137081

    Purpose

    bacterial Himar1 mini-transposon donor plasmid

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar1 mini-transposon with chloramphenicol resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC18T-mini-Tn7T-Gm-Pc-tdTomato

    Plasmid

    #199250

    Purpose

    Fluorescent labelling of bacterial cells with tdTomato

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized tdTomato gene
    

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTara:500

    Plasmid

    #60717

    Purpose

    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a_Pro-IL-1β

    Plasmid

    #214340

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1B (IL1B Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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