32,963 results
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pOT2
Plasmid#14460DepositorInsertGfp-UV
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisA-PPARalpha(aa101-468)
Plasmid#13574DepositorInserthuman peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
Tagsx6His N-terminal, enterokinase cleavage siteExpressionBacterialMutationdeleted residues 1-100.Available SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Smad7-HA
Plasmid#13506DepositorAvailable SinceDec. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30 SUMO-1 G97
Plasmid#13381DepositorInsertSUMO-1, G97 (SUMO1 Human)
TagsHisExpressionBacterialMutationG97 mutant: lacks 4 amino acids from the C-termin…Available SinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 EGFP-Sumo2 GG
Plasmid#13384DepositorInsertSumo2 GG (SUMO2 Human)
TagsEGFP and GSTExpressionBacterialMutationMature SUMO2GG: diglycine at amino acid positions…Available SinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
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883 pET15b PTEN
Plasmid#10936DepositorAvailable SinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTUM4
Plasmid#230905PurposePlasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coliDepositorInsertsExpressionBacterialAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
prhaBAD-pA-Hia5
Plasmid#222303PurposePlasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertpA-Hia5
Tags6His and protein AExpressionBacterialMutationcodon optimized for bacterial expressionPromoterrhaBADAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLemon
Plasmid#219399PurposeConstitutive expression of mLemon in E. coliDepositorInsertmLemon
ExpressionBacterialPromoterproDpAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
TagsbdSUMOExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-pA-M.EcoGII
Plasmid#192873PurposepA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578DepositorInsertpA-M.EcoGII
Tags3X Flag TagExpressionBacterialPromoterT7Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHybrid-Electra2
Plasmid#179475PurposeExpresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cellsDepositorInsertsElectra2
mScarlet-I
ExpressionBacterial and MammalianAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR0306 CcaCas13b His6-TwinStrep-SUMO
Plasmid#182687PurposeExpresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purificationDepositorInsertCcaCas13b
TagsSUMOExpressionBacterialPromoterT7Available SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHyo182
Plasmid#173136PurposeExpresses pheS_A294G in e.coliDepositorInsertpheS_A294G
ExpressionBacterialAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
plRL58
Plasmid#166886PurposeOptimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIMcpf1
Plasmid#153034PurposeFor CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.DepositorInsertAscpf1
ExpressionBacterialAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recX-sacB
Plasmid#158718PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.DepositorInsertrecX
ExpressionBacterialPromoterPmycAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only
