30,260 results

1. pQE30-His-RF3

   Plasmid

   #124131

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 3 (prfC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS413-GAL1-yEGFP1

   Plasmid

   #40235

   Depositor

   Michael Benton

   Insert

   yEGFP1
   

   Use

   Tags

   HIS3MX6

   Expression

   Bacterial

   Mutation

   Promoter

   GAL1

   Available since

   Oct. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDuEx2-NIR-GECO1

   Plasmid

   #113680

   Purpose

   Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NIR-GECO1
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-2TK-14-3-3 eta GST

   Plasmid

   #13277

   Depositor

   Michael Yaffe

   Article

   Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

   Insert

   14-3-3 eta (YWHAH Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pET11a-Z-NspGFP

   Plasmid

   #40729

   Depositor

   Brian McNaughton

   Article

   Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

   Insert

   N super positive GFP - Leucine Zipper
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-c2X

   Plasmid

   #75286

   Purpose

   Expresses proteins in the cytoplasm as fusions to maltose-binding protein

   Depositor

   Paul Riggs

   Article

   Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

   Type

   Empty backbone

   Use

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 18, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28a-mAmetrine-CNA35

   Plasmid

   #61604

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mAmetrine and CNA35
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMyNT

   Plasmid

   #42191

   Depositor

   Annabel Parret, Matthias Wilmanns

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   ColE1

   Available since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJKR-O-mphR

    Plasmid

    #62570

    Purpose

    Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJMP1339

    Plasmid

    #119271

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbS8k-RFP

    Plasmid

    #35276

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. TccCas13a Twin SUMO

    Plasmid

    #199754

    Purpose

    Expresses codon optimized thermostabile TccCas13a in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    TccCas13a
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRS-BG42-GFP

    Plasmid

    #220202

    Purpose

    mini-Tn7 GFP expression vector - pBG42 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1m-ELP

    Plasmid

    #170763

    Purpose

    EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    EF1m-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRS-AraC-GFP

    Plasmid

    #220199

    Purpose

    mini-Tn7 GFP expression vector - AraC/pBAD promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    AraC/pBAD

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRS-XylS-GFP

    Plasmid

    #220200

    Purpose

    mini-Tn7 GFP expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    XylS/pM

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP3666

    Plasmid

    #222352

    Purpose

    Expression vector with GFP expressed under PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli.

    Depositor

    Jason Peters

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PabstBR

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP64T Activin betaB (DM#108)

    Plasmid

    #15045

    Depositor

    Douglas Melton

    Article

    Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

    Insert

    Activin Beta b (inhbb Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pTP1112

    Plasmid

    #104158

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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