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32,113 results

Showing: 5201 - 5240 of 32113 results

  2. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

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  4. pETM6-G6-vioABE-4A6-vioC

    Plasmid
    #73439
    Purpose
    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6 and P4A6 (orthogonal T7-lac variants)
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73439-plasmid-map-sequence-id-135338

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  14. pBHM2404

    Plasmid
    #173441
    Purpose
    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    mNeonGreen-CBP-2XFLAG-NAT1
    Tags
    CBP-2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    173441-plasmid-map-sequence-id-341226

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  15. pMBEC2

    Plasmid
    #187596
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187596-plasmid-map-sequence-id-367535

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  16. pGEX4T1-Opt WWP1

    Plasmid
    #179327
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1
    Depositor
    Arthur Haas
    Insert
    WWP1 (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Available Since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179327-plasmid-map-sequence-id-358706

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  20. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid
    #218193
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF
    Depositor
    Florence Pojer
    Insert
    PNGaseF
    Tags
    tufA-His-CBD-3C
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-354
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218193-plasmid-map-sequence-id-431798

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  22. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. pET28-SBP-TEV

    Plasmid
    #36943
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHistag-SBP-TEV
    Expression
    Bacterial
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    36943-plasmid-map-sequence-id-46249

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  26. FKBP1B

    Plasmid
    #73241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FKBP1B (FKBP1B Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. pGGD_RT_SRDX

    Plasmid
    #136976
    Purpose
    Goldengate/Greengate module EAR-repression domain
    Depositor
    Klaus Theres
    Insert
    SRDX
    Expression
    Bacterial
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136976-plasmid-map-sequence-id-272093

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  32. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87703-plasmid-map-sequence-id-176992

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  37. pRSF-G1(CysCNP)RS

    Plasmid
    #225054
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Chem 102385
    Insert
    tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    225054-plasmid-map-sequence-id-446766

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Showing: 5201 - 5240 of 32113 results