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31,059 results

Showing: 7151 - 7200 of 31059 results

  4. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40787-plasmid-map-sequence-id-68902

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  5. p007ampGFP

    Plasmid
    #58535
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Expression
    Bacterial
    Promoter
    T5 between two lac operators
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58535-plasmid-map-sequence-id-90574

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  6. pXZ13lacZalpha

    Plasmid
    #160229
    Purpose
    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160229-plasmid-map-sequence-id-314764

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  9. AWP-031

    Plasmid
    #213967
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.
    Depositor
    Alexander Westermann
    Article
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Synthetic, Segatella bryantii)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213967-plasmid-map-sequence-id-429166

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  12. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  13. pSCW02

    Plasmid
    #72301
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72301-plasmid-map-sequence-id-255198

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  14. pGGG-Sr43

    Plasmid
    #186974
    Purpose
    Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).
    Depositor
    Brande Wulff
    Article
    Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:
    Insert
    Sr43
    Tags
    None
    Expression
    Bacterial and Plant
    Mutation
    None
    Promoter
    Natice
    Available Since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    186974-plasmid-map-sequence-id-382711

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  17. pConRef-2H12

    Plasmid
    #221182
    Purpose
    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221182-plasmid-map-sequence-id-437177

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  20. EPHA7 (2REI)

    Plasmid
    #25148
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    epha7.590.899 (EPHA7 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pET15b/EYFP-GgVcl 884-1066-ECFP FRET

    Plasmid
    #46179
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (VCL Chicken)
    Tags
    ECFP, EYFP, and His
    Expression
    Bacterial
    Mutation
    aa 884-1066 (Vt, tail domain)
    Promoter
    T7
    Available Since
    July 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    46179-plasmid-map-sequence-id-198213

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  38. pJK407

    Plasmid
    #72421
    Purpose
    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    lipase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Available Since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72421-plasmid-map-sequence-id-134675

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  41. pTCF200 Mouse beta-2 microglobulin

    Plasmid
    #180462
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Mouse beta-2 microglobulin
    Tags
    BSP41
    Expression
    Bacterial
    Available Since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180462-plasmid-map-sequence-id-355083

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  43. BRDT

    Plasmid
    #38898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRDT (BRDT Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. pJMP8823

    Plasmid
    #220901
    Purpose
    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo
    Depositor
    Jason Peters
    Article
    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003
    Insert
    dCas9-novo
    Use
    CRISPR
    Tags
    3xMyc
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220901-plasmid-map-sequence-id-437054

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  49. pET-Bst-LF-Idaho

    Plasmid
    #170469
    Purpose
    Expresses His-tagged Bst-LF-Idaho in E. coli
    Depositor
    Jonathan Schmid-Burgk
    Insert
    Bst-LF-Idaho
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170469-plasmid-map-sequence-id-338354

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  50. pCK301

    Plasmid
    #87767
    Purpose
    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Insert
    PrhaBAD-sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87767-plasmid-map-sequence-id-177279

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Showing: 7151 - 7200 of 31059 results