30,249 results

1. pMSP3545

   Plasmid

   #46888

   Purpose

   Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

   Depositor

   Gary Dunny

   Article

   Bryan et al Plasmid. 2000 Sep;44(2):183-90.

   Type

   Empty backbone

   Use

   Nisin-inducible expression; gram-positive bacteri…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PnisA

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnEA-vH_His-TEV-SEPT2_SEPT6

   Plasmid

   #174497

   Purpose

   bacterial co-expression of human SEPT2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRBC-sfGFP wild type

   Plasmid

   #174075

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEVOL_PylRS(AF)-tRNA

   Plasmid

   #223511

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y306A; Y384F

   Promoter

   araBAD, rrnB, and glnS and proK

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbA6c_PsulA_sfGFP

   Plasmid

   #224587

   Purpose

   SOS response reporter

   Depositor

   Ophelia Venturelli

   Article

   Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PsulA

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28HIS-hAPE1

   Plasmid

   #70757

   Purpose

   bacterial expression of human APE1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   APEX1 (APEX1 Human)
   

   Use

   Tags

   6-HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lac operon

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-DAXX-His

   Plasmid

   #175782

   Purpose

   DAXX protein for bacteria expression system

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRAD1

   Plasmid

   #47697

   Purpose

   General cloning vector for use in E. coli or D. radiodurans

   Depositor

   Mary Lidstrom

   Article

   Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   repU

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDx_mScarlet-I3

    Plasmid

    #189757

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB2-mKate2

    Plasmid

    #104009

    Purpose

    Plasmid encoding mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mKate2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, V45A, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_GNAI2_G-alpha

    Plasmid

    #109791

    Purpose

    Protein expression and purification of GNAI2_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI2_G-alpha (GNAI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQmod3C-GG

    Plasmid

    #191350

    Purpose

    Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIB185

    Plasmid

    #90196

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAT-WT-Myc

    Plasmid

    #177717

    Purpose

    ER protein alpha-1-antitrypsin (WT), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNDGG054

    Plasmid

    #231323

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCPP5372

    Plasmid

    #128733

    Purpose

    Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

    Depositor

    Alan Collmer

    Article

    Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNCS-mCyRFP1

    Plasmid

    #84545

    Purpose

    Bacterial expression of mCyRFP1

    Depositor

    Michael Lin

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMDH

    Plasmid

    #133129

    Purpose

    A recombinant plasmid expressing mdh (malate dehydrogenase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    malate dehydrogenase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    malate dehydrogenase promoter

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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