31,870 results
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Plasmid#224211PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only
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Ecoli PancACe
Plasmid#215712PurposeExpresses acetyl-CoA biosensor in EcoliDepositorInsertPancACe
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-FLAG-dN6-His
Plasmid#22143DepositorInsertFlag-clpXdeltaNLinkedHexamer-His6
TagsFlag, His6, and L20 LinkersExpressionBacterialAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
TagsHisExpressionBacterialMutationE54K and L372PAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-hGSDMD
Plasmid#111559PurposeExpress human GSDMD in E.coliDepositorAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
ExpressionBacterialAvailable SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
spNW50
Plasmid#173486PurposeExpresses spNW50 in Bl21DepositorInsertspNW50
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29a-YS14
Plasmid#66890PurposePolycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)DepositorInsertsHis6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin
TagsH2A, H2B, H3, H4, His6 tag, and ThrombinExpressionBacterialPromoterT7Available SinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGTM_YR375_SUMO_Protease_001
Plasmid#190063PurposeExpresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusionDepositorInsertULP1_YEAST (ULP1 Budding Yeast)
Tags8X His-MBP (Maltose Binding Protein)ExpressionBacterialMutationConstruct contains residues 347 – 621PromoterT7 lacAvailable SinceJan. 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
ExpressionBacterialPromoterglnS and pBADAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
TagsHis tagExpressionBacterialPromoterT7Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
TagsHalo and His6ExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-cOVA
Plasmid#25097DepositorInsertcytoplasmic ovalbumin
ExpressionBacterial and MammalianAvailable SinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-H6-FGAm (FlincG3)
Plasmid#49202PurposeFluorescent reporter for cGMPDepositorInsertFlincG3
TagsHis tag + other tagsExpressionBacterial, Insect, and Mamm…MutationMet to Lys in cpEGFP region similar to CGaMP3PromoterCMVAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-1UAG
Plasmid#82500PurposeExpresses GFP with 1 UAG codon at the amino acid position 3DepositorInsertGreen Fluorescent Protein with 1 UAG codon
ExpressionBacterialMutationAdded an UAG codon at the amino acid position 3PromoterAraBADAvailable SinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE1
Plasmid#186804PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo
Plasmid#239948PurposeExpression of MFSD6 loop 3 tagged with rabbit IgG Fc2DepositorInsertFc2-MFSD6(L3)
ExpressionBacterial and MammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGM1192
Plasmid#115683PurposeE. coli-Streptomyces shuttle vector for gene expressionDepositorInsertmCherry, aac(3)IV, tsr
TagsStrep-tagII on mCherry, codon optimizedExpressionBacterialMutationmCherry codon optimized for StreptomycesPromoterSF14*Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SV40LT
Plasmid#136616PurposeDox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cellsDepositorInsertSV40 large T antigen
UseLentiviralExpressionBacterialAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only