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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Publication

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pY001 (pFnCpf1_full)

Plasmid

#69973

Purpose

Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus

Use

CRISPR

Expression

Bacterial

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX-EZH2

Plasmid

#28060

Depositor

Mien-Chie Hung

Publication

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Tags

GST

Expression

Bacterial

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEM-LIN28

Plasmid

#16350

Depositor

James Thomson

Publication

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens lin-28 homolog (C. elegans) (LIN28A Human)

Expression

Bacterial

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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psfTn5

Plasmid

#79107

Purpose

bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

Depositor

Sten Linnarsson

Insert

transposase 5

Tags

His

Expression

Bacterial

Mutation

E54K and L372P

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pet30b-5M SrtA

Plasmid

#51140

Purpose

expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

Depositor

Hidde Ploegh

Publication

Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

Insert

S.aureus SrtA 5M

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pET9d-hnRNP-A1

Plasmid

#23026

Depositor

Douglas Black

Insert

hnRNPA1 (HNRNPA1 Human)

Expression

Bacterial

Promoter

T7

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Publication

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Tags

14Histidine-TEV

Expression

Bacterial

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Publication

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Publication

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pTRKH3-slpGFP

Plasmid

#27168

Depositor

Michela Lizier

Publication

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

surface (S)-layer protein promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pET11phiC31poly(A)

Plasmid

#18942

Depositor

Michele Calos

Publication

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GSK3 beta pGEX

Plasmid

#15898

Depositor

Jim Woodgett

Insert

GSK3 beta (GSK3B Human)

Tags

GST

Expression

Bacterial

Available since

Nov. 1, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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T444T

Plasmid

#113081

Purpose

Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

Depositor

Tibor Vellai

Publication

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

RNAi

Expression

Bacterial

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pRSET-mSA-EGFP

Plasmid

#39862

Depositor

Sheldon Park

Publication

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin fused to EGFP

Tags

6xHis, EGFP, and FLAG

Expression

Bacterial

Promoter

T7

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNH-TrxT

Plasmid

#26106

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis, TEV cleavage site, and TrxA

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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GST-Pin1

Plasmid

#19027

Depositor

Michael Yaffe

Publication

Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

Insert

Pin1 (PIN1 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pET23b-Casp3-His

Plasmid

#11821

Depositor

Guy Salvesen

Publication

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Publication

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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