32,115 results
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Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
ExpressionBacterialPromoterPpro1Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBL090J
Plasmid#195045PurposecCadC-CCB3.5 under the control of PBADDepositorInsertcCadC-CCB3.5
ExpressionBacterialAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-EXO1b
Plasmid#68267PurposeBacterial expression of human EXO1DepositorInsertEXO1 (EXO1 Human)
TagsMxe intein/chitin binding domainExpressionBacterialMutationb form, codons 1-846. Please see depositor commen…PromoterT7Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-PR/PRM-GFP
Plasmid#80859PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
ExpressionBacterialPromoterLambda Pr and Lambda PrmAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS Wnt-6HA
Plasmid#17378DepositorAvailable SinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
BRD2
Plasmid#39074PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
6his-WT gusA-pET28a+
Plasmid#61156PurposeN -terminal His tagged wild-type GUS expression vectorDepositorInsertgusA
Tags6x HisExpressionBacterialPromoterT7Available SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFloat.cspA_v1
Plasmid#133071PurposeEntry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-His6 and His6-3CExpressionBacterialPromotercspAAvailable SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EIF5A
Plasmid#25260PurposeBacterial expression for structure determination; may not be full ORFDepositorInserteIF5A:S15-A151 (EIF5A Human)
TagsHisExpressionBacterialMutationContains amino acids S15-A151Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOTIII.EFGP.IGR3.6
Plasmid#40866DepositorAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACλCI
Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
ExpressionBacterialPromoterlac UV5Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2PPN
Plasmid#234190PurposeHeterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS-lpp0.2-K64A
Plasmid#201789Purposemutant of PadR; transcriptional regulator can respond to p-coumaric acidDepositorAvailable SinceJuly 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_ESRRB_Hormone-recep
Plasmid#109909PurposeProtein expression and purification of ESRRB_Hormone-recepDepositorInsertESRRB_Hormone-recep (ESRRB Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNLS-2xBFP(4C)
Plasmid#176151PurposeEncodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.DepositorInsertNLS-2xBFP(4C)
Tags6xHis tag and SV40 nuclear localization sequence …ExpressionBacterialMutationCompared with EGFP, the two BFP domains have the …PromoterPtrcAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestDD04Neo
Plasmid#26386DepositorTypeEmpty backboneExpressionBacterial and WormAvailable SinceOct. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
PB6K-mcherry-iATPsnFR1.1
Plasmid#227239PurposeInducible ATP sensor for Pseudomonas putidaDepositorInsertiATPsnFR1.1
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
C1-82 pComb3xss anti-cFos nanobody
Plasmid#145829PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-PUM1-HD
Plasmid#17543DepositorAvailable SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_14
Plasmid#59952PurposeTriple split (at 67, 601-SZ)DepositorInsertThree-fragment T7* RNA polymerase
ExpressionBacterialMutationN/APromoterPTacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP1
Plasmid#129504PurposeExpresses AvicFP1 constitutively in E. coli (most strains)DepositorInsertAvicFP1
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CJ8421_04975 Rptr2 recording plasmid
Plasmid#194211PurposeCJ8421_04975 Rptr2 targeted DNA sequenceDepositorInsertCJ8421_04975 Rptr2 DNA target
ExpressionBacterialAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ACPs (from S. pneumonia)
Plasmid#63687PurposeInducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniaeDepositorInsertacpS
TagsHisExpressionBacterialPromoterT7Available SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recAmu-sacB
Plasmid#158717PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.DepositorInsertrecA
ExpressionBacterialMutationR61CAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1415
Plasmid#84828PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Cdk9 T186A
Plasmid#28101DepositorInsertCdk9 (CDK9 Human)
TagsFLAGExpressionBacterial and MammalianMutationContains a T186A mutation in Cdk9Available SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-3WJdB-T
Plasmid#87311PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.DepositorInsertThree-way Junction dimeric Broccoli aptamer
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS:TVA-mCherry
Plasmid#187823PurposeExpression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.DepositorInsertTVA-mCherry
ExpressionBacterialPromoter5xUASAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-1xGFP-6xHis
Plasmid#196972PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tagDepositorAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG310
Plasmid#115611Purposesurface-displayed Ag2, Arabinose-inducible; medium-copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b-gRNA-1
Plasmid#184834PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b-gRNA-NT
Plasmid#184835PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-NT
Plasmid#184838PurposeExpression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.DepositorInsertCo-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1MycHis/HIP1
Plasmid#29590DepositorAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB
Plasmid#26607DepositorAvailable SinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mCardinal2
Plasmid#52631Purposebacterial expression vector with far-red FP mCardinal2DepositorInsertmCardinal2
ExpressionBacterialAvailable SinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only