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32,161 results

Showing: 641 - 680 of 32161 results

  3. pGEX6P1-N-HA

    Plasmid
    #119756
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and HA
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119756-plasmid-map-sequence-id-233665

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  4. pGL004

    Plasmid
    #215336
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    hyper-pSpank
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215336-plasmid-map-sequence-id-426101

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  5. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

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  10. pNB22

    Plasmid
    #103059
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Tags
    none
    Expression
    Bacterial
    Promoter
    hmpAp
    Available Since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103059-plasmid-map-sequence-id-199053

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  23. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61737-plasmid-map-sequence-id-190162

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  25. pSW002-Pc-TorA(sp)-mNeonGreen

    Plasmid
    #205020
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorA-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205020-plasmid-map-sequence-id-407993

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  26. pCR2.1-InC

    Plasmid
    #83959
    Purpose
    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
    Depositor
    Kai-Shun Chen
    Insert
    Internal Control sequence
    Expression
    Bacterial
    Available Since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83959-plasmid-map-sequence-id-160468

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  29. KAT2B

    Plasmid
    #39002
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KAT2B (KAT2B Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available Since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

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  34. pTP1172

    Plasmid
    #104161
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104161-plasmid-map-sequence-id-200072

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  39. cBEST2

    Plasmid
    #234658
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    kasO*17tss and kasOp*
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234658-plasmid-map-sequence-id-492748

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  40. cBEST3

    Plasmid
    #234659
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and kasO17tss
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234659-plasmid-map-sequence-id-492747

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Showing: 641 - 680 of 32161 results