32,126 results
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Plasmid#89662Purposeexpression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT21
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q
Plasmid#84352PurposeExpression of the human Huntingtin Exon1 protein containing 43Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT32
Plasmid#89667Purposeexpression of doubleTAL staple protein dT32 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT32
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSC226
Plasmid#216375PurposexhNup358-Nb2t (+3Cys) Nanobody productionDepositorInsertxhNup358-Nb2t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT16
Plasmid#89659Purposeexpression of doubleTAL staple protein dT16 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT16
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK
Plasmid#126554PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-Rab29
Plasmid#186021Purpose2xHis-Rab29DepositorInsert2xHis-Rab29 (RAB29 Human)
ExpressionBacterialAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23106-RiboJ-UTR1-E0040m-B0015)
Plasmid#197674PurposeExpresses GFP under the control of J23106 and RiboJ-UTR1DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23100-RiboJ-BCD2-E0040m-B0015)
Plasmid#197661PurposeExpresses GFP under the control of J23100 and RiboJ-BCD2DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23103-RiboJ-BCD2-E0040m-B0015)
Plasmid#197667PurposeExpresses GFP under the control of J23103 and RiboJ-BCD2DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-SP44s-GUS
Plasmid#172430PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidaseDepositorInsertbeta-D-glucuronidase
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterSP44sAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT006
Plasmid#182716PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloningDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsGFP in cloning site of crRNA for easier screening…ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBH532_MBP-Cas14b1 expression
Plasmid#112505PurposeExpresses 10xHis-MBP fused to Cas14b1 under t7 promoterDepositorInsertCas14b1
UseCRISPRTagsMBPExpressionBacterialPromoterT7Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD deltahexa
Plasmid#169714PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deletedDepositorInserthnRNPA1 (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2/hGGA3(GAT)
Plasmid#79436PurposeExpresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coliDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET-PesaRlux
Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-luxCDABE-term
UseSynthetic BiologyExpressionBacterialPromoterPesaRAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX 6P1 Cyclin A (555)
Plasmid#61844Purposebacterial expression of GST cyclin ADepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-oriT
Plasmid#32556DepositorInsertoriT
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIE
Plasmid#171083PurposeCo-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permissionDepositorTagsHisExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
23 pCOLI_A_N-SUMO_C_STREP
Plasmid#160259PurposeN-term His-SUMO 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags2xStrepII, 6xHis, and SUMOExpressionBacterialPromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-Pspac
Plasmid#112778PurposePromoter repressed by LacI (not natively produced in B. subtilis 168)DepositorArticleInsertPspac promoter
ExpressionBacterialAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAT224
Plasmid#134892PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAPK3
Plasmid#73235PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK3 (MAPK3 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00069:Pkinase 42-330PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCT3-Halo
Plasmid#175322PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SETD7
Plasmid#40746PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceNov. 14, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-luxOFP
Plasmid#229945PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)DepositorInsertluxOFP
TagsHis-tagExpressionBacterialPromoteraraBADAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTKIP-neo
Plasmid#41064DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68
Plasmid#153527PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#68DepositorInsertSb#68
ExpressionBacterialPromoterpBADAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs3-SH2
Plasmid#46506DepositorInsertSocs3 (SOCS3 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 22-142PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
p361_DG
Plasmid#234683PurposeGolden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiTDepositorTypeEmpty backboneTagsHis6-3C-3XFLAG-SUMO and Thrombin-HiBiTExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pBig
Plasmid#51962PurposeExpression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in DictyosteliumDepositorInsertYellow Cameleon-Nano15
ExpressionBacterialAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pROP-IP-FBFP
Plasmid#122138PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)DepositorInsertFBFP
ExpressionBacterialPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV125
Plasmid#63191PurposepRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-AtUBC8-S-AtUBA1-S
Plasmid#222714PurposeExpression AtUBA1-S and AtUBC8-S in in bacterial cellsDepositorAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-HIS-FLAG-AtUBQ11
Plasmid#222720PurposeExpression HIS-FLAG-AtUBQ11 in in bacterial cellsDepositorAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-jREX-GECO1
Plasmid#113941PurposeBacterial expression of red fluorescent Ca2+ sensor jREX-GECO1DepositorInsertjREX-GECO1
Tags6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterialAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only