29,744 results

1. pET-28c(+)-YTHDF3

   Plasmid

   #102276

   Purpose

   For bacterial expression of His-tagged human YTHDF3

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDF3 (YTHDF3 Human)
   

   Use

   Tags

   6X HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBS834 H14-SUMO-TDP43 WT-TEV-mCherry

   Plasmid

   #133320

   Purpose

   Bacterial expression of TDP43 with WT CTD

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-EPN-24

   Plasmid

   #79939

   Purpose

   PH-Late1-I3-01-myc

   Depositor

   Neil King

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN-24
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHH0103_GRB2-1/2

   Plasmid

   #91480

   Purpose

   Protein expression and purification of human SH3 domain construct GRB2-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   GRB2-1/2 (GRB2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-mH6-Cas12g1

   Plasmid

   #120879

   Purpose

   Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12g1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. T777T

   Plasmid

   #113082

   Purpose

   Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJ251-GERC

   Plasmid

   #47441

   Purpose

   Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.

   Depositor

   George Church

   Insert

   Gene Expression Reporter Construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAX1

   Plasmid

   #117397

   Purpose

   allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBBR1_DVK_AE

   Plasmid

   #188946

   Purpose

   Level 1 destination vector containing pBBR1 origin of replication and CIDAR AE scars

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. T4 Lysozyme WT*

    Plasmid

    #18111

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No cysteines: C54T, C97A

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184741

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

    Plasmid

    #117728

    Purpose

    Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAJM.011

    Plasmid

    #108529

    Purpose

    TetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    TetR + PTet*-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZac2.1 gfaABC1D-LifeAct-GFP

    Plasmid

    #190199

    Purpose

    Expresses the small actin binding peptide, LifeAct, with a GFP reporter

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    LifeAct-eGFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-PIPK1 gamma 87

    Plasmid

    #22300

    Depositor

    Pietro De Camilli

    Article

    Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

    Insert

    human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSG019

    Plasmid

    #126502

    Purpose

    Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

    Depositor

    Ute Romling

    Article

    Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

    Insert

    disaggregase ClpG with 6xHis-tag
    

    Use

    Tags

    C-terminal 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAWP78-PVCpnf_pvc13-ALFA

    Plasmid

    #198301

    Purpose

    Produces PVCs retargeted with ALFA tag

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-ALFA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSEVA228-pro4IUPi

    Plasmid

    #122018

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pro4

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEXP5-NT/pT7 LuxI

    Plasmid

    #193626

    Purpose

    Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LuxI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge