30,753 results

1. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

   Plasmid

   #105235

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH6HTC_CCT2-Halo

   Plasmid

   #175337

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCT2 (CCT2 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPomyces-FnCpf1

   Plasmid

   #129554

   Purpose

   Contains codon-optimized FnCpf1 and gRNA for streptomyces

   Depositor

   Fong Tian Wong, Mingzi M. Zhang

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   FnCpf1
   

   Expression

   Bacterial

   Promoter

   rpsL(XC)-BbsI

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET42b-ABE7.10

   Plasmid

   #120398

   Purpose

   Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   Junjiu Huang

   Article

   Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

   Insert

   ABE7.10
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mammalian codon-optimized

   Promoter

   T7

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDSG288

   Plasmid

   #115607

   Purpose

   surface-displayed Ag3, used in composability experiments; low copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKAR2-Br512g3.1

   Plasmid

   #171200

   Purpose

   Expresses Br512g3.1 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al medRxiv 2022.08.09.22278605

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (vHP47…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTD-NTwinStrep_Sm

   Plasmid

   #45937

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBx-Spas-sgRNA-Gm

   Plasmid

   #105232

   Purpose

   Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a Halotag-CNA35

    Plasmid

    #131128

    Purpose

    Halotag-CNA35 peptide for Collagen labelling

    Depositor

    Samantha Stehbens

    Article

    Ju et al bioRxiv 2022

    Insert

    Halotag-CNA35
    

    Tags

    Halotag

    Expression

    Bacterial

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLG163

    Plasmid

    #124365

    Purpose

    IPTG inducible system (gfpmut2 with Ppsank) for expression in B. subtilis

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pspank

    Available Since

    July 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKnockinDonor-human-p16INK4A-P2A-mCherry

    Plasmid

    #137026

    Purpose

    donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

    Depositor

    Chunliang Li

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

    Insert

    p16INK4A-knockin-homology arm-P2A-mCherry
    

    Tags

    P2A-mCherry

    Expression

    Bacterial

    Promoter

    no promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCE27

    Plasmid

    #174405

    Purpose

    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDSG358

    Plasmid

    #115599

    Purpose

    surface-displayed Ag1; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Expression

    Bacterial

    Mutation

    See depositor comments below

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST17-UbE2M

    Plasmid

    #15798

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2M (UBE2M Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pET28b-DiCas7-11-His

    Plasmid

    #234482

    Purpose

    Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLADE_ONLY_A

    Plasmid

    #168051

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Expression

    Bacterial

    Promoter

    J23101**

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX4T moesin 1-577

    Plasmid

    #11637

    Depositor

    Vijaya Ramesh

    Article

    Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

    Insert

    moesin (MSN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSM019

    Plasmid

    #128291

    Purpose

    asymmetric plasmid partitioning E. coli - regulatory plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Para - lp-sfyfp-parB
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1.Gα13-LgB106

    Plasmid

    #134364

    Purpose

    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα13-LgB106 (GNA13 Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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