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pFGL821

Plasmid

#58223

Purpose

For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

hygromycin B phosphotransferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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H6-auxilin

Plasmid

#62571

Purpose

6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria

Depositor

Ernst Ungewickell

Article

Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304

Insert

Auxilin

Tags

6XHis

Expression

Bacterial

Mutation

*see comment below

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
H6-AP180

Plasmid

#63006

Purpose

expresses the adaptor protein AP180 in bacteria

Depositor

Ernst Ungewickell

Article

Norris et al J Biol Chem. 1995 Jan 6;270(1):214-7.

Insert

AP180 (Snap91 Rat)

Tags

His-tag

Expression

Bacterial

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only
pMemoryArray

Plasmid

#60585

Purpose

An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

MemoryArray

Expression

Bacterial

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_4

Plasmid

#60576

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_4

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTE101

Plasmid

#59396

Purpose

MethyloBrick cloning vector, no promoter; KmR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

none

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-MSI1(FL)

Plasmid

#60358

Purpose

express mouse MSI1 in mammalian cells

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (FL) (Msi1 Mouse)

Expression

Bacterial

Promoter

CMV

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDI

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-KDM4A double Tudor

Plasmid

#59699

Purpose

Contains histone modification interacting domain (KDM4A double Tudor)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

KDM4A double Tudor (KDM4A Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_8

Plasmid

#59948

Purpose

PT7 GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PT7

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX3X-RanBP2dFG

Plasmid

#53120

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2553-2838)

Promoter

tac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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RAP1B

Plasmid

#55665

Purpose

expression of mouse RAP1B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP1B (Rap1b Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pOPINEneo-3C-GFP

Plasmid

#53534

Purpose

pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

Depositor

Ray Owens

Insert

GFP

Tags

GFP-His8

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Tags

GST

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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CMV:: HA-hM4Dnrxn

Plasmid

#52525

Purpose

Expresses N-terminal HA tagged hM4Dnrxn

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

HA-hM4Dnrxn (CHRM4 Human, Synthetic)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCOM4

Plasmid

#49798

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMal-E4rGel

Plasmid

#51280

Purpose

Expresses a fibronectin fusion with gelonin targeting EGFR in bacteria

Depositor

Dane Wittrup

Article

Pirie et al J Biol Chem. 2011 Feb 11;286(6):4165-72.

Insert

E4rGel

Expression

Bacterial

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGMCtKq28-TSC10M1-sspB

Plasmid

#49526

Purpose

Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Expression

Bacterial

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse Dicer

Plasmid

#50357

Purpose

Mouse Dicer cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

Dicer1 (Dicer1 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAND

Plasmid

#49377

Purpose

Expression of AND gate in bacteria

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

AND gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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