29,744 results

1. pBBR2k-GFPuv

   Plasmid

   #106383

   Purpose

   Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYTK003

   Plasmid

   #65110

   Purpose

   Encodes ConL1 as a Type 1 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   ConL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15-His-∆NGlpG

   Plasmid

   #155131

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   insert codes for GlpG residues 87-276

   Promoter

   T7

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. ath5:GFP-CAAX

   Plasmid

   #105958

   Purpose

   transgenesis, EGFP membrane

   Depositor

   Caren Norden

   Article

   Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

   Insert

   CAAX
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30a-His6-ABP-LOXCAT

   Plasmid

   #139971

   Purpose

   Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DHX9A

   Plasmid

   #42456

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DHX9A (DHX9 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pES002 MBP-GSK3β_S9A-HA-His

   Plasmid

   #196184

   Purpose

   Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Tags

   HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-SENP1 (catalytic domain)

   Plasmid

   #16356

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP1 catalytic domain (SENP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 419 to 644 catalytic domain of human S…

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDDGFP-B

   Plasmid

   #40287

   Depositor

   Robert Campbell

   Article

   Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

   Insert

   ddGFP-B
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pET22b-6h-GST-TEVp

    Plasmid

    #172887

    Purpose

    Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Insert

    TEV protease
    

    Use

    Tags

    GST solubility tag and HHHHHH

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p405ADH1

    Plasmid

    #15966

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    p405ADH1 (ADH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    ADH1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbA5a-sufCDSUB

    Plasmid

    #195055

    Purpose

    Expresses SUF operon from B. subtilis from IPTG-inducible promoter

    Depositor

    Greg Bokinsky

    Article

    D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

    Insert

    sufCDSUB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AK2

    Plasmid

    #38829

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK2 (AK2 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pBSV2_PflaB-mCherryBb

    Plasmid

    #118243

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF420

    Plasmid

    #123659

    Purpose

    Produces cofactor F420 in E. coli

    Depositor

    Colin Jackson

    Article

    Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

    Inserts

    CofD
    FbiB
    FbiD
    FbiC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_R0040

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. L4440_BioBrick-SCR-sgRNA-B

    Plasmid

    #177812

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    Scrambled sgRNA targeting sequences
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-MHL: nsp16_SARS-CoV-2

    Plasmid

    #167850

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pET28-MHL: nsp10_SARS-CoV-2

    Plasmid

    #167851

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp10

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pBS-hsFlt3L

    Plasmid

    #25074

    Depositor

    Maria Castro

    Article

    Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

    Insert

    human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-28a(+)-FGF2-K128N

    Plasmid

    #120284

    Purpose

    Produce human recombinant FGF2 K128N in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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