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pET20b-His6-LONP1-Walker B mutant (E591A)

Plasmid

#247938

Purpose

Expression of human LONP1 with methionine 115 as its first residue (mature mitochondrial-matrix localized form) and Walker B mutant (E591A)

Depositor

Gabriel Lander

Article

Shin et al Nat Commun. 2021 May 28;12(1):3239. doi:

Insert

LONP1 (LONP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

encodes aa 115-959, with E591A

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc

Plasmid

#196241

Purpose

N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag

Tags

GST tag and myc tag

Expression

Bacterial

Mutation

amino acid 35 lysine is replaced with glutamic ac…

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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MZB053

Plasmid

#217835

Purpose

Bacterial expression of human AP3B1 (1-677) and AP3M1 AP-3 core hemicomplex

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Inserts

AP3B1 (AP3B1 Human)
AP3M1 (AP3M1 Human)

Tags

GST

Expression

Bacterial

Mutation

Removed residues 678-1094 from AP3B1

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (catalytically active, self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204795

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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NBla_1.2_EmrE_TMD4_3P_G90V_G97V

Plasmid

#207847

Purpose

BLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_EmrE_TMD4_3P

Plasmid

#207846

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_GpA_wt

Plasmid

#207842

Purpose

BLaTM-System GpA wt positive control in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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CBLa_1.2_GpA_wt

Plasmid

#207843

Purpose

BLaTM-System GpA wt positive control in CBLa 1.2 vector

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAP01

Plasmid

#186260

Purpose

sfGFP under light light responsive PEL222 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Insert

sfGFP

Expression

Bacterial

Promoter

PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251_ Ptrc.x.tetO2::D-HicDH

Plasmid

#185456

Purpose

CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

D-HicDH from Lactobacillus paracasei DSM 20008

Expression

Bacterial

Promoter

Ptrc.x.tetO2

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB402-er-mCherry

Plasmid

#186288

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTD103-ARG1

Plasmid

#106475

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLux

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET Flag TEV LIC cloning vector (2L-T)

Plasmid

#29715

Depositor

Scott Gradia

Type

Empty backbone

Tags

FLAG-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pDD57

Plasmid

#119882

Purpose

Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_GFP+

Expression

Bacterial

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Sperm whale Mb 64Y68F in pVP80K

Plasmid

#86956

Purpose

Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

Depositor

John Olson

Insert

Mb 64Y68F

Expression

Bacterial

Mutation

64Y68F

Promoter

T5

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GST-GGA3-VHS-GAT

Plasmid

#173719

Purpose

VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

Depositor

Juan Bonifacino

Article

Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

Insert

GGA3 (GGA3, GGA2, GGA1 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFGM1

Plasmid

#64949

Purpose

source of GmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-GmR-FRT

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN

Plasmid

#178032

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Promoter

T7LacI

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-GST-FBW7 alpha

Plasmid

#197456

Purpose

The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Use

In vitro translation

Tags

GST

Expression

Bacterial

Mutation

See depositor comments below

Promoter

T7

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe-SH1446

Plasmid

#112507

Purpose

Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

Depositor

Simon Newstead

Article

Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

Insert

POT family transporter

Tags

C terminal TEV GFP octa-His affinity tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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