29,744 results

1. pWZ403

   Plasmid

   #208113

   Purpose

   mSwAP-In marker cassette 1 with mScarlet-PuroTK

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mScarlet-PuroTK
   

   Use

   Tags

   P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. His_Naa80d in pTYB12

   Plasmid

   #188455

   Purpose

   Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   Naa80 (NAA80 Human)
   

   Use

   Tags

   intein-CBD, 6xHis

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFN18A-HaloTag-SpyCatcher

   Plasmid

   #206041

   Purpose

   HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   HaloTag and SpyCatcher-8His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBHM2329

   Plasmid

   #171686

   Purpose

   pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   C. neoformans U6

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-TRSter

   Plasmid

   #78865

   Purpose

   Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

   Depositor

   Elias Arnér

   Article

   Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

   Insert

   Thioredoxin Reductase (Txnrd1 Rat)
   

   Use

   Tags

   Mutated E. coli-type SECIS element (3’ end of ins…

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLEXIN A1

   Plasmid

   #58237

   Purpose

   Riboprobe for in vitro transcription of Plexin A1

   Depositor

   Christiana Ruhrberg

   Article

   Bouvree et al Circ Res. 2012 Aug 3;111(4):437-45. doi:

   Insert

   Plexin A1 (Plxna1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pT7RNAP

   Plasmid

   #170101

   Purpose

   expresses T7 RNAP

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   T7-RNA polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HS-Ras-mKate2

   Plasmid

   #105951

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   Ras
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEK22

   Plasmid

   #210588

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   BdH3pro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   BdH3pro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDFDuet-nsp7-nsp8

    Plasmid

    #159092

    Purpose

    Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

    Depositor

    Seth Darst

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Inserts

    NSP7
    NSP8
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD33.lon

    Plasmid

    #22145

    Depositor

    Robert Sauer

    Article

    Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

    Insert

    Lon (lon E. coli K12)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None, WT gene

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HS-H2B-RFP

    Plasmid

    #105953

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    H2B
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PGEX-NEDD4

    Plasmid

    #45043

    Depositor

    Shiaw-Yih Lin

    Article

    Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

    Insert

    NEDD4-1 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pQLinkN

    Plasmid

    #13670

    Depositor

    Konrad Buessow

    Article

    Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

    Insert

    multi cloning site
    

    Use

    Co-expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. luxA+luxB/pRSETB Duet

    Plasmid

    #197581

    Purpose

    Expressing luxA and luxB under dual T7 promoter

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxB
    

    Use

    Tags

    T7 tag and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ykgMO-IGS-sfGFP

    Plasmid

    #207231

    Purpose

    Calprotectin sensing promoter with sfGFP reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET51b-His-TEV-CLIP-tag_fast

    Plasmid

    #167275

    Purpose

    Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBbB6c-GFP

    Plasmid

    #35356

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ibpAB-GFP

    Plasmid

    #109390

    Purpose

    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pibpAB-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PP-GSK3

    Plasmid

    #25133

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    cgd4_240:C29838
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits