30,753 results

1. pTHSSe_40

   Plasmid

   #109239

   Purpose

   PTac driving TALEsp2

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   TALEsp2
   

   Expression

   Bacterial

   Promoter

   PTac

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC201plus

   Plasmid

   #164227

   Purpose

   Integrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression

   Depositor

   Silvia Cardona

   Article

   Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Rhamnose-inducible

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. [pSK131] pCOLADuet p14|MP1

   Plasmid

   #136145

   Purpose

   bacterial expression of p14 and MP1

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   p14 and MP1
   

   Expression

   Bacterial

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFCiKm7

   Plasmid

   #167521

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinRAM-KmR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB1A3_GFP_dT33

   Plasmid

   #89668

   Purpose

   expression of doubleTAL staple protein dT33 with N-terminally fused cycle3-GFP

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   GFP_dT33
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK_kanR_proJK1_PAD

   Plasmid

   #60630

   Purpose

   Constitutively expresses PAD (phenolic acid decarboxylase)

   Depositor

   Timothy Whitehead

   Article

   Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

   Insert

   Phenolic acid decarboxylase padC (padC )
   

   Expression

   Bacterial

   Mutation

   K146Y

   Promoter

   proJK1

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pramr-GFP

   Plasmid

   #194148

   Purpose

   Contains a RamR-regulated promoter expressing GFP

   Depositor

   Andrew Ellington

   Article

   d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

   Insert

   GFP
   

   Expression

   Bacterial

   Promoter

   Pramr

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTHSSe_39

   Plasmid

   #109238

   Purpose

   PTac driving TALEsp1

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   TALEsp1
   

   Expression

   Bacterial

   Promoter

   PTac

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30-2-PHGDH

   Plasmid

   #83907

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Tags

   6x His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pETM41_KPNB1_HEAT

    Plasmid

    #178494

    Purpose

    Bacterial expression of human domain with His-tag and MBP tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    KPNB1_HEAT (KPNB1 Synthetic, Human)
    

    Tags

    His-MBP

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-32a(+)-TGFB1m

    Plasmid

    #120286

    Purpose

    Produce human recombinant mTGFB1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β1
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promotor

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUS252

    Plasmid

    #127674

    Purpose

    Expresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    Free Use GFP (fuGFP)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    June 24, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1GFP-His-GFP-TEV-cs-ATG3

    Plasmid

    #197472

    Purpose

    Bacterial Expression of human ATG3

    Depositor

    James Hurley

    Insert

    ATG3 (ATG3 Human)
    

    Tags

    His-GFP

    Expression

    Bacterial

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpyB-GFP

    Plasmid

    #220190

    Purpose

    sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQFT-mNG-Pcon-Scar

    Plasmid

    #203678

    Purpose

    pQFT-mNG with constitutively expressed mScarlet-I

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    mScarlet-I
    

    Expression

    Bacterial

    Promoter

    PQJ and Phyb18

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQFT-mNGsacB

    Plasmid

    #203676

    Purpose

    pQFT with mNeonGreen and sacB under control of PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    sacB
    

    Expression

    Bacterial

    Mutation

    Has synonymous (silent) mutations to remove BsrGI…

    Promoter

    PQJ

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. QUAS-0-T7-GFP_ColE1

    Plasmid

    #171649

    Purpose

    QUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-0-T7
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. T7-LacO-QF_p15A

    Plasmid

    #171651

    Purpose

    Expresses QF activator protein in BL21(DE3) E. coli. Contains p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QF-T7 Stop
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. QUAS-5-T7-GFP_ColE1

    Plasmid

    #171653

    Purpose

    QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-5-T7
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge