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31,059 results

Showing: 7801 - 7840 of 31059 results

  3. pAGH42

    Plasmid
    #107249
    Purpose
    Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    vipp1-gfp
    Use
    Suicide vector for destined for double homologous…
    Tags
    mGFPmut3
    Expression
    Bacterial
    Promoter
    native promoter of sll0617
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107249-plasmid-map-sequence-id-207556

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  7. p404GAL1

    Plasmid
    #17417
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17417-plasmid-map-sequence-id-8592

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  12. pGBW-m4092677

    Plasmid
    #153883
    Purpose
    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stop
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    wt;handful of SNPs as watermarks
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153883-plasmid-map-sequence-id-307322

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  18. pTSlb-N748D

    Plasmid
    #60732
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60732-plasmid-map-sequence-id-99421

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  19. pDipA-TEV-His12

    Plasmid
    #137042
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137042-plasmid-map-sequence-id-275698

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  22. GABARAPL1

    Plasmid
    #25215
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh57.003.111 (GABARAPL1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. Rec-PhoA/CsgA

    Plasmid
    #170787
    Purpose
    Recombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinase
    Depositor
    Urartu Seker
    Article
    Ahan et al Chembiochem. 2019 Jul 15;20(14):1799-180
    Inserts
    csgA (csgA )
    phoA (phoA )
    Use
    Synthetic Biology
    Tags
    HHHHHH
    Expression
    Bacterial
    Promoter
    Inverted proD with Bxb1recognition sites and Inve…
    Available Since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    170787-plasmid-map-sequence-id-343131

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  26. pET14b-dabAB2

    Plasmid
    #133013
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Promoter
    t7
    Available Since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133013-plasmid-map-sequence-id-262987

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  27. GPX3

    Plasmid
    #38899
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX3 (GPX3 Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. pRSF-Ivy-his-hLYZ

    Plasmid
    #83920
    Purpose
    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.
    Depositor
    Karl Griswold
    Article
    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do
    Inserts
    Ivy
    lysozyme (LYZ Human)
    Tags
    Ivy protein has C-terminal hisx6 tag
    Expression
    Bacterial
    Mutation
    Ivy leader sequence deleted and replaced.
    Promoter
    T7 and t7
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    83920-plasmid-map-sequence-id-160356

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  40. pJK234

    Plasmid
    #73324
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC
    Depositor
    T. J. Kappock
    Inserts
    violacein biosynthesis protein VioA
    violacein biosynthesis protein VioB
    violacein biosynthesis protein VioC
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73324-plasmid-map-sequence-id-135032

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Showing: 7801 - 7840 of 31059 results