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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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32,126 results

Showing: 7801 - 7840 of 32126 results

  1. K980

    Plasmid
    #129762
    Purpose
    Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129762-plasmid-map-sequence-id-263587

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  3. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225139-plasmid-map-sequence-id-460505

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  12. pDIV537

    Plasmid
    #177704
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177704-plasmid-map-sequence-id-352590

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  17. pUC19-link-sfGFP-FLAG:Kan

    Plasmid
    #211833
    Purpose
    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.
    Depositor
    Peng Chen
    Insert
    sfGFP-FLAG-Kan
    Expression
    Bacterial
    Mutation
    NA
    Available Since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211833-plasmid-map-sequence-id-418068

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  22. pOPS0254

    Plasmid
    #115506
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115506-plasmid-map-sequence-id-224294

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  27. CD Ugi1

    Plasmid
    #177194
    Purpose
    Greengate entry vector pGGC000 modified to contain bladderwort insulator 1
    Depositor
    Wayne Parrott
    Article
    Park et al bioRxiv 2021.11.04.467280
    Insert
    Utricularia gibba insulator 1
    Expression
    Bacterial
    Available Since
    June 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177194-plasmid-map-sequence-id-361543

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  30. pAIP4_2WWE

    Plasmid
    #234210
    Purpose
    Heterologous protein expression of PI3K-C2-gamma in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2WWE (PIK3C2G Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234210-plasmid-map-sequence-id-464175

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  38. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  40. pTriEx-mPA-GFP-PA-Rac1

    Plasmid
    #22022
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Tags
    6X His and mPA-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 7801 - 7840 of 32126 results