29,771 results

1. pRha-ABE7.10-SpCas9-NG

   Plasmid

   #170663

   Purpose

   Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells

   Depositor

   David Liu

   Article

   Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10

   Insert

   ABE7.10-SpCas9-NG
   

   Use

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   pRhaBAD

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBEI-6411

   Plasmid

   #47050

   Purpose

   BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
   

   Use

   Synthetic Biology; Bglbrick

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC3-FGFR1

   Plasmid

   #222611

   Purpose

   Expression of MAC3-tagged FGFR1

   Depositor

   Markku Varjosalo

   Article

   Liu et al Methods Mol Biol. 2023;2690:281-297. doi

   Insert

   FGFR1 (FGFR1 Human)
   

   Use

   Tags

   MAC3

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. UEV1

   Plasmid

   #25619

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   UEV1 (UBE2V1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172505

   Purpose

   Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHJL4

   Plasmid

   #199379

   Purpose

   Encodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   jGCaMP7f::SL2::mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no mutations

   Promoter

   Available since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pMRE-Himar-131

   Plasmid

   #185923

   Purpose

   Himar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein gene

   Depositor

   Mitja Remus-Emsermann

   Article

   Stocks et al Bacteria 2022, 1(1), 56-65

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD-pHScarlet

   Plasmid

   #191258

   Purpose

   Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet

   Depositor

   Robert Campbell

   Insert

   pHScarlet
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAWP78

   Plasmid

   #61263

   Purpose

   Small IncP-based broad host range replicating vector.

   Depositor

   Mary Lidstrom

   Article

   Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AviTag-SpyCatcher

    Plasmid

    #72326

    Purpose

    Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    AviTag-SpyCatcher
    

    Use

    Tags

    N-term His6 tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HMGCS1

    Plasmid

    #38844

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HMGCS1 (HMGCS1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pRha-ABE8e-NRCH

    Plasmid

    #165417

    Purpose

    For bacterial purification of ABE8e-NRCH protein

    Depositor

    David Liu

    Insert

    ABE8e-NRCH
    

    Use

    Tags

    8xHIS and BP-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    pRhaBAD

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Human RNF144A (16-228)

    Plasmid

    #110752

    Purpose

    Bacterial Expression of RNF144A RBR domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    RNF144A (RNF144A Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCON1-ProB.gfp

    Plasmid

    #112516

    Purpose

    ProB driven gfpmut3 expression with synTT

    Depositor

    Olivia Steele-Mortimer

    Article

    Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

    Insert

    ProB gfpmut3 TT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProB

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYTK-DN5

    Plasmid

    #180286

    Purpose

    NatR multi-gene yeast expression vector (GFP-dropout)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS' - GFP - ConRE' - NatR - CEN6/ARS4
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAWP78-PVCpnf_pvc13-truncated

    Plasmid

    #198286

    Purpose

    Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-truncated
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMAL-EFSep

    Plasmid

    #34627

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    EF-Sep (tufB E. coli)
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Available since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pCPP5616

    Plasmid

    #128727

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    avrPtoB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-CV-SNAP25

    Plasmid

    #168031

    Purpose

    Expresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-SNAP25(A141-G206)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC18T-mini-Tn7T-hph-Ptac

    Plasmid

    #218364

    Purpose

    Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge