30,756 results

1. pET28_6xHis-PARP1delBRCT

   Plasmid

   #173938

   Purpose

   Bacterial expression of PARP1 lacking BRCT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delBRCT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 374-520; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_GAS7-1/1

   Plasmid

   #91301

   Purpose

   Protein expression and purification of human SH3 domain construct GAS7-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   GAS7-1/1 (GAS7 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINO_NbH11-H4

   Plasmid

   #155364

   Purpose

   Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

   Insert

   H11-H4
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pEERM4 Cm

   Plasmid

   #64026

   Purpose

   Plasmid for integration into neutral site 2 of Synechocystis 6803

   Depositor

   Pia Lindberg

   Article

   Englund et al ACS Synth Biol. 2015 Jul 13.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PnrsB

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET23 P53DBD

   Plasmid

   #174503

   Purpose

   express P53 DNA binding domain (94-312) in E.coli without tag

   Depositor

   Stewart Loh

   Article

   Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

   Insert

   P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
   

   Tags

   none

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a+GFP-CAMSAP3 CKK

   Plasmid

   #59033

   Purpose

   expresses CKK domain of CAMSAP3 in E. coli

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP3 CKK domain (CAMSAP3 Mouse)
   

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pRSFDuet-v2 RsTAL At4CL

   Plasmid

   #126524

   Purpose

   expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

   Depositor

   Andrew Woolley

   Article

   Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

   Inserts

   tyrosine ammonia lyase
   p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115216

   Purpose

   Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   Pin2Cas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMW41: pET-His-MBP-SUMO-R2Bm-SII

   Plasmid

   #209290

   Purpose

   Expresses the R2Bm ORF in E. coli

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2
   

   Tags

   His, MBP, StrepII, and bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCis GFPuv-SS Himar1 A7

    Plasmid

    #84686

    Purpose

    Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

    Depositor

    Ulrike Munderloh

    Article

    Munderloh et al Intracellular Pathogens II: Rickettsiale

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBWB294

    Plasmid

    #140636

    Purpose

    pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTOX11

    Plasmid

    #127456

    Purpose

    Encodes MqsR toxin. Gent-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    mqsR toxin
    

    Expression

    Bacterial

    Promoter

    pRha

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas1+2

    Plasmid

    #72676

    Purpose

    Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

    Depositor

    Udi Qimron

    Article

    Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

    Insert

    Cas1, Cas2
    

    Expression

    Bacterial

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pClodANL-FGF4

    Plasmid

    #107874

    Purpose

    Bacterial expression of FGF4-NanoLuc

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    FGF4 (FGF4 Synthetic)
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Bacterial

    Promoter

    pro D

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Parp14-c012

    Plasmid

    #173099

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    W1003-A1190

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBx-Spas-sgRNA-Kan

    Plasmid

    #105233

    Purpose

    Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP1237

    Plasmid

    #119262

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLN-AIDA-Tyr1

    Plasmid

    #192831

    Purpose

    IPTG inducible AIDA-Tyrosinase expression

    Depositor

    André Studart

    Article

    Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

    Insert

    AIDA-Tyr1
    

    Expression

    Bacterial

    Promoter

    pL-lac

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4046887

    Plasmid

    #145730

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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