32,156 results
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Plasmid#21497DepositorTagsHisExpressionBacterialMutationnoneAvailable SinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only
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pMN252
Plasmid#32177DepositorInsertrpsL, FRT-hyg-FRT
ExpressionBacterialAvailable SinceNov. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS415-SPT6
Plasmid#10993DepositorAvailable SinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
ExpressionBacterialPromoterE . coli insulated waaH gene promoter, low phosp…Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM cCol2 (CT#617)
Plasmid#13943DepositorAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
SNX29-PX (269-391)
Plasmid#119108PurposeBacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)DepositorInsertSNX29-PX (269-391)
TagsGSTExpressionBacterialPromotertacAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BmNG
Plasmid#232207PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTyDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PmNG
Plasmid#232201PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTyDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM8315
Plasmid#213988PurposePrpsM driven expression of mCherry2-LDepositorInsertmCherry2-L
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1_CylLS_CylM
Plasmid#208760PurposeExpresses modified cytolysin S (mCylLS) in E. coliDepositorInsertsCylLS
CylLM
TagsHisx6 TagExpressionBacterialPromoterT7Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZ8-Prp
Plasmid#74065PurposepZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanRDepositorInsertPropionate inducible promoter
UseSynthetic BiologyExpressionBacterialPromoterprpAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Q11
Plasmid#185781PurposeEncodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Q11
ExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-gfp_pJMP2768
Plasmid#208889PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
ExpressionBacterialAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.gfp
Plasmid#112516PurposeProB driven gfpmut3 expression with synTTDepositorInsertProB gfpmut3 TT
ExpressionBacterialPromoterProBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CFL1 (CFL1A-c002)
Plasmid#98237PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCFL1A (CFL1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00241:Cofilin_ADF 27-148PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pID1072
Plasmid#164869PurposeThe pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.DepositorInsertphiKO2 cB repressor
ExpressionBacterialAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM263-USP7
Plasmid#196365Purposeprotein expression of the GST-tagged catalytic domainDepositorInsertUSP7 catalytic domain
TagsHis6-GST-TEVExpressionBacterialMutationcontains only S207-E560PromoterT7Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVV108
Plasmid#215338PurposeExpresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPSalTTCAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA334
Plasmid#214746PurposeHigh copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
ExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Caspase-6
Plasmid#100102PurposeBacterial expression of Caspase-6DepositorAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoL-OG241
Plasmid#72841PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.DepositorInsertPatoL
ExpressionBacterialPromoterPatoLAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PA-TagRFP-pBad
Plasmid#62793PurposeLocalization: Protein Expression Vector , Excitation:N/A, Emission:N/ADepositorTypeEmpty backboneTags6xHis and TagRFPExpressionBacterialMutationnoneAvailable SinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAL-pck_NT
Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterptacAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-KKHSaCas9-BlastR
Plasmid#107189PurposeConfers constitutive expression of KKH SaCas9.DepositorInsertKKH SaCas9 (NEWENTRY )
ExpressionBacterialMutationE782K/N968K/R1015HPromoterChicken ß-ActinAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTBXh-2
Plasmid#26078DepositorTypeEmpty backboneTagsHisExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMK90
Plasmid#13323DepositorInsertgyrA
ExpressionBacterialAvailable SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMS26
Plasmid#32295DepositorTypeEmpty backboneUseBacterial gene additionExpressionBacterialPromoterrhaBAvailable SinceSept. 26, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINMSYB
Plasmid#41118DepositorTypeEmpty backboneExpressionBacterial, Insect, and Mamm…Available SinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHG5
Plasmid#188253PurposeIntegrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGGG-Sr43
Plasmid#186974PurposeSr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).DepositorInsertSr43
TagsNoneExpressionBacterial and PlantMutationNonePromoterNaticeAvailable SinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 661-1014Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSN007
Plasmid#102440PurposeFor PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)DepositorInsert7SK paired gRNA insert
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-pT3-tetR-sfGFP
Plasmid#140871PurposeT3 promoter driven synthesis of TetR-sfGFPDepositorInsertTetR-sfGFP expressed with T3 promoter
UseSynthetic BiologyTagssfGFPExpressionBacterialPromoterT3Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PXWABS62(E11-TEV)
Plasmid#123157PurposeFor TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-tDepositorInsertParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
UseSynthetic BiologyExpressionBacterialPromoterParsR-ABS62, Pecf11Available SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBluescript GPX-1
Plasmid#13038DepositorInsertGPX-1 (GPX1 Human)
ExpressionBacterialMutationAdditional GCG upstream of codon 11. Codon 92 is …Available SinceNov. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCPP5238
Plasmid#128711PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopG1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 KD
Plasmid#13322DepositorInsertPINK1 kinase domain (PINK1 Human)
TagsGSTExpressionBacterialMutationAmino acids 112-496; Kinase dead: K219D/D362A/D3…Available SinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
ELK3_pet28a
Plasmid#131651PurposeBacterial expression of ETS gene ELK3DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDipA-TEV-His12
Plasmid#137042Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only