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32,156 results

Showing: 8761 - 8800 of 32156 results

  4. SpyTag003-OspC

    Plasmid
    #159998
    Purpose
    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells
    Depositor
    Mark Howarth
    Article
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Insert
    SpyTag003-OspC (20-209) C129S
    Tags
    His6-tag and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    C129S mutation improves expression level.
    Promoter
    T7
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159998-plasmid-map-sequence-id-316165

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  11. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

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  14. pJMP8823

    Plasmid
    #220901
    Purpose
    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo
    Depositor
    Jason Peters
    Article
    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003
    Insert
    dCas9-novo
    Use
    CRISPR
    Tags
    3xMyc
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220901-plasmid-map-sequence-id-437054

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  19. pCX0393

    Plasmid
    #205984
    Purpose
    Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Inserts
    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    truncated CRISPR array
    Tags
    14xHis and TwinStrep-tag
    Expression
    Bacterial
    Promoter
    T7 (proteins), pJ23119 (crRNA)
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205984-plasmid-map-sequence-id-416238

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  26. pGBW-m4046814

    Plasmid
    #145601
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145601-plasmid-map-sequence-id-292512

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  27. HSPA1LA

    Plasmid
    #42429
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSPA1LA (HSPA1L Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. PTPRB

    Plasmid
    #39165
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRB (PTPRB Human)
    Tags
    GST-PreScission
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. pUC19-T7-SdB-T

    Plasmid
    #87307
    Purpose
    Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Stabilized dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87307-plasmid-map-sequence-id-174665

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  34. pGlu57

    Plasmid
    #211560
    Purpose
    Amber Suppressor tRNA for Glutamate
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAGlu
    Expression
    Bacterial
    Promoter
    Synthetic (Miller 1986)
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211560-plasmid-map-sequence-id-438928

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  35. pGly57

    Plasmid
    #211561
    Purpose
    Amber Suppressor tRNA for Glycine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAGly
    Expression
    Bacterial
    Promoter
    Synthetic (Miller 1986)
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211561-plasmid-map-sequence-id-438930

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  36. pHis57

    Plasmid
    #211562
    Purpose
    Amber Suppressor tRNA for Histidine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAHis
    Expression
    Bacterial
    Promoter
    Synthetic (Miller 1986)
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211562-plasmid-map-sequence-id-438922

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  37. pLeu57

    Plasmid
    #211563
    Purpose
    Amber Suppressor tRNA for Leucine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALeu
    Expression
    Bacterial
    Promoter
    Synthetic (Miller 1986)
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211563-plasmid-map-sequence-id-417239

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  38. pLys57-1

    Plasmid
    #211564
    Purpose
    Amber Suppressor tRNA for Lysine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Expression
    Bacterial
    Promoter
    Synthetic (Miller 1986)
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211564-plasmid-map-sequence-id-438925

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  39. pLys57-2

    Plasmid
    #211565
    Purpose
    Amber Suppressor tRNA for Lysine (Methionine-derived)
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Expression
    Bacterial
    Promoter
    Synthetic (Miller 1986)
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211565-plasmid-map-sequence-id-421056

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  40. pLys57-3

    Plasmid
    #211566
    Purpose
    Amber Suppressor tRNA for Lysine (Isoleucine-derived)
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Expression
    Bacterial
    Promoter
    Synthetic (Miller 1986)
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211566-plasmid-map-sequence-id-421057

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Showing: 8761 - 8800 of 32156 results