31,591 results
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Plasmid#198296PurposeProduces PVCs retargeted with HA tagDepositorInsertPVCpnf_pvc13-HA
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-HF1-NLS-6xHis (gbr2102)
Plasmid#181744Purposebacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY-HF1
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…PromoterT7Available SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCool-mRbx1-His-hCul1-CTP
Plasmid#29519DepositorTagsGST, His (before thrombin), and Thrombin Cut Site…ExpressionBacterialAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cre-HiBiT
Plasmid#198318PurposeLoads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains PnfDepositorInsertPdp1_NTD-Cre-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP1B
Plasmid#73241PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
ptac::ccdB
Plasmid#15922DepositorInsertccdB (ccdB E. coli)
ExpressionBacterialAvailable SinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAJM.474
Plasmid#108526PurposeLuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.DepositorInsertLuxR + PLuxB-YFP
ExpressionBacterialMutationVanR: T49I A111V P179SAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorInsertNbVHH05-ALFA-His (UBE2J1 Human)
TagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH
Plasmid#83907Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
Tags6x His tagsExpressionBacterialPromoterT7Available SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-5
Plasmid#228965PurposeFor bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-5
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)/MBP-His-PINK1
Plasmid#38005DepositorAvailable SinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMR-A-3-Sasg
Plasmid#214349PurposeExpresses T7-RNAP, and cloning backbone for sgRNADepositorInsertT7-RNAP
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_HECT
Plasmid#187719PurposeExpress GST-tagged NEDD4L HECT domain in bacterial cellsDepositorInsertNEDD4L residues 594-975 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-593Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
MP6-SlugABEN-738
Plasmid#214348PurposeExpresses N-terminus of ABE, and untargeted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
ExpressionBacterialAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT120a17-sg-del
Plasmid#214347PurposeExpresses pIIIDepositorInsertgIII-xLuxAB
ExpressionBacterialAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag
Plasmid#187356PurposeBacterial expression and refolding of gp100 pMHCDepositorInserthHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
ExpressionBacterialPromoterT7Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)_beta2M (MHC Class I light)
Plasmid#187357PurposeBacterial expression and refolding of gp100 pMHCDepositorInsertbeta2M (MHC Class I light)
ExpressionBacterialPromoterT7Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAD39_mIRG1_4-462_pvp008
Plasmid#124873PurposeExpresses mouse CAD (Irg1) in E.coliDepositorInsertcis-aconitate decarboxylase (Acod1 Mouse)
TagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-3 and 463-end.PromoterT7Available SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_AP2B1_adaptin
Plasmid#178467PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only