33,128 results

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pIB165

Plasmid

#90188

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-B CFP

Plasmid

#108858

Purpose

Encodes for the fluorescent protein CFP

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

CFP

Expression

Bacterial

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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HS-mKate2

Plasmid

#105945

Purpose

heat shock inducible transgenesis, cytosolic mKate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

mKate2

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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phage UbiC SNAP-KDEL

Plasmid

#104982

Purpose

Lentiviral vector expressing SNAP-KDEL for ER labeling

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

SNAP-KDEL

Use

Lentiviral

Tags

Secretion signal

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mGFPmut3

Plasmid

#103981

Purpose

Plasmid encoding mGFPmut3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, S72A, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-FKBPx5-His

Plasmid

#103785

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5-His

Tags

EYFP, His-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDB023f1

Plasmid

#99218

Purpose

Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD4] T7RNAP(S12*,S203*)

Expression

Bacterial

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pmVirD2

Plasmid

#90314

Purpose

mVirD2 expression plasmid

Depositor

Bjorn Hogberg

Article

Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

Insert

A. tumefaciens VirD2 from aa 1 to 204

Tags

His6x tag

Expression

Bacterial

Promoter

T7/T5 IPTG inducible

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET-KIX (586-672)

Plasmid

#99336

Purpose

Bacterial expression of mouse CBP KIX domain

Depositor

Peter Wright

Article

Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

Insert

CBP KIX (586-672) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-GFP-AURKA

Plasmid

#99875

Purpose

Expression of GFP-AURKA for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Bacterial

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAV126

Plasmid

#63192

Purpose

pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_12E

Plasmid

#98652

Purpose

Expresses MBP-tagged full length phosphomimetic FUS

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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K84M kinase-dead Nuak1-3xflag

Plasmid

#97219

Purpose

Plasmid expressing kinase-dead mutant Nuak1

Depositor

Huda Zoghbi

Article

Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

Insert

Nuak1 (NUAK1 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Kinase-dead K84M

Promoter

T7

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-TelgRNA_PuroR

Plasmid

#84782

Purpose

Expresses telomere-specific sgRNA.

Depositor

Heinrich Leonhardt

Article

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Insert

telomere-specific sgRNA

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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371-GFP

Plasmid

#97030

Purpose

His6-GFP-tev-yORF

Depositor

Chris Jeans

Type

Empty backbone

Tags

GFP fusion, His6 tag, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Ppr_Cas13a_WT

Plasmid

#91872

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

PprCas13a (PprC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS Citrine

Plasmid

#91761

Purpose

Bacterial expression of a fluorescent protein, Citrine

Depositor

Erik Rodriguez, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Insert

Citrine

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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