30,803 results

1. pCpf1-sp

   Plasmid

   #122186

   Purpose

   Bacterial genome editing plasmid with spectinomycin resistance marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYTK002

   Plasmid

   #65109

   Purpose

   Encodes ConLS as a Type 1 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   ConLS
   

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-IG-mSA

   Plasmid

   #80706

   Purpose

   Expresses monomeric streptavidin (mSA) in bacterial cells

   Depositor

   Matthieu Sainlos

   Article

   Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

   Insert

   Monomeric streptavidin
   

   Tags

   10xHis tag and TEV site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFNF

   Plasmid

   #22687

   Depositor

   Robert Benezra

   Article

   Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

   Insert

   Neo*
   

   Use

   Mouse Targeting

   Expression

   Bacterial and Mammalian

   Mutation

   FRT-pPGK-pEM7-Neo*-bpA-FRT

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMemoryArray

   Plasmid

   #60585

   Purpose

   An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   MemoryArray
   

   Expression

   Bacterial

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pST-2K

   Plasmid

   #44564

   Purpose

   for ectopic constitutive expression of two mycobacterial proteins in one vector

   Depositor

   Vinay Nandicoori

   Article

   Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

   Type

   Empty backbone

   Use

   E.coli-mycobacteria shuttle vector

   Tags

   Flag tag and His tag

   Expression

   Bacterial

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p2CT-His-MBP-Lba_Cas13a_WT

   Plasmid

   #91867

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbaCas13a (LbaC2c2) WT
   

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEEK2

   Plasmid

   #83492

   Purpose

   Expression plasmid for conjugational transfer into cyanobacteria

   Depositor

   Pia Lindberg

   Article

   Miao et al Metab Eng Commun. 2017 Jul 29;5:45-53. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Strep-tag

   Expression

   Bacterial

   Promoter

   Ptrc core

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FLAG-Matr3 delZnF1

   Plasmid

   #32883

   Depositor

   Yossi Shiloh

   Article

   Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

   Insert

   MATR3 Matrin3 (MATR3 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   deleted amino acids 283-322

   Promoter

   CMV

   Available Since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

10. FLAG-Matr3 delZnF2

    Plasmid

    #32884

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    deleted amino acids 793-833

    Promoter

    CMV

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid

    #183027

    Purpose

    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-6His-SUMO-Nsp10

    Plasmid

    #154959

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET-21(+)Tm9D8* TrpB

    Plasmid

    #207517

    Purpose

    Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    Tryptophan synthase β-subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Pin1

    Plasmid

    #40773

    Depositor

    Dustin Maly

    Insert

    Pin1 (PIN1 Human)
    

    Tags

    6His and TEV

    Expression

    Bacterial

    Mutation

    Starting Met removed

    Promoter

    T7

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas-pML-1B_6xHis-Cas12m

    Plasmid

    #192282

    Purpose

    MmCas12m fused to His-tag for protein purification

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12m
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pALS2-sfGFP 150TAG

    Plasmid

    #197574

    Purpose

    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p2.0VPI.EGFP

    Plasmid

    #40868

    Depositor

    Harold Gainer

    Article

    Ponzio et al PLoS One. 2012;7(11):e48860. doi: 10.137

    Insert

    mouse vasopressin promoter
    

    Use

    AAV

    Expression

    Bacterial and Insect

    Promoter

    mouse vasopressin

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KE-005

    Plasmid

    #133440

    Purpose

    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4A1 (EIF4A1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6972 pPROEX-HTb-SSA1

    Plasmid

    #1231

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    Ssa1 (SSA1 Budding Yeast)
    

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Available Since

    Aug. 9, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pH6HTC_CCT3-Halo

    Plasmid

    #175322

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCT3 (CCT3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge