30,803 results

1. pET-15b PAI-RBP

   Plasmid

   #45390

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pJB166

   Plasmid

   #86998

   Purpose

   CRISPR/Cas9 in fission yeast using fluoride selection

   Depositor

   Julien Berro

   Article

   Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE136

   Plasmid

   #118490

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Expression

   Bacterial

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. IVT-nCas9(H840A)

   Plasmid

   #207455

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   H840A
   

   Expression

   Bacterial

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FsC_pET29b

   Plasmid

   #215405

   Purpose

   Expression construct for FsC gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   FsC
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-iNOS

   Plasmid

   #19295

   Depositor

   Charles Lowenstein

   Article

   Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89

   Insert

   inducible nitric oxide synthase (Nos2 Mouse)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-FOLD6

   Plasmid

   #186547

   Purpose

   FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   FOLD6
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

   Promoter

   araB

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. BBa_J72114-BBa_J72104

   Plasmid

   #40783

   Depositor

   Christopher Anderson

   Article

   Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

   Insert

   RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ
   

   Use

   Luciferase and Synthetic Biology; Phagemid

   Expression

   Bacterial

   Available Since

   Jan. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

   Plasmid

   #114072

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
   

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   E174R, S542R, and K548R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET11b-SpyCatcher-HA2

    Plasmid

    #165991

    Purpose

    SpyCatcher-fused influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-HA2
    

    Tags

    Foldon, HisTag, and SpyCatcher

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLG025 (DUF4297 + HerA)

    Plasmid

    #157903

    Purpose

    Expresses the DUF4297 + HerA defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DUF4297 + HerA
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28_mouse-Caspase-1_enzymatic-domain

    Plasmid

    #183385

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404) (Casp1 Mouse)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-RT005-HaloGPM

    Plasmid

    #163385

    Purpose

    2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFDH

    Plasmid

    #131706

    Purpose

    Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

    Depositor

    Ron Milo

    Article

    Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

    Insert

    formate dehydrogenase
    

    Tags

    his-tag

    Expression

    Bacterial

    Available Since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmMaple3-CAM

    Plasmid

    #101148

    Purpose

    Fluorescent protein for superresolution imaging

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    E. coli codon optimized mMaple3
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE133

    Plasmid

    #118487

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Expression

    Bacterial

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST HP1 beta

    Plasmid

    #24075

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 beta (CBX1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pQF-lacZ

    Plasmid

    #48094

    Purpose

    Cumate-inducible expression of lacZ

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Insert

    E. coli lacZ
    

    Expression

    Bacterial

    Promoter

    PQ5

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid

    #83485

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJUMP23-1A(sfGFP)

    Plasmid

    #126970

    Purpose

    Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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