29,771 results

1. pTXB1-FIS

   Plasmid

   #51564

   Purpose

   Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

   Depositor

   Sankar Adhya, Francisco Malagon

   Insert

   FIS
   

   Use

   Tags

   Intein-Chiting Binding Domanin

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-UbE2D3

   Plasmid

   #15784

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   see Depositor Comments below

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMMB67EH-Spy

   Plasmid

   #90102

   Purpose

   IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   YcgR (ycgR )
   

   Use

   Broad-host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM33_EIF4E_eiF4E

   Plasmid

   #178474

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   EIF4E_eiF4E (EIF4E Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-Synaptojanin 1-145

   Plasmid

   #22291

   Depositor

   Pietro De Camilli

   Article

   Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

   Insert

   Synaptojanin 1_145 (SYNJ1 Human)
   

   Use

   Tags

   Flag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Tn7-143

   Plasmid

   #118559

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP8045

   Plasmid

   #220900

   Purpose

   Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   tnsABCD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD/HisB-iRFP670

   Plasmid

   #45453

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   araBAD

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21b-Caspase-3(C163S)

   Plasmid

   #90088

   Purpose

   Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   C163S

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. As0

    Plasmid

    #123158

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS67, PhrpLE, PrinA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE165

    Plasmid

    #118513

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Reb_206 aka PAS629

    Plasmid

    #71234

    Purpose

    Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Use

    Tags

    mNeonGreen to second copy RebB, soluble mCherry a…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-SPA2G231V

    Plasmid

    #42079

    Depositor

    Christine Garcia

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    Surfactant Protein -A2 (SFTPA2 Human)
    

    Use

    Lentiviral; Plenti

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Changed Glycine 231 to Valine

    Promoter

    CMV

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pGEX-4T1-RhoA G17A

    Plasmid

    #69357

    Purpose

    expression of nucleotide free RhoA in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G17A

    Promoter

    tac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-2T-RhoA-wt

    Plasmid

    #12959

    Depositor

    Gary Bokoch

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

17. SpyCatcher002-oTri

    Plasmid

    #124662

    Purpose

    Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTri
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcNB1

    Plasmid

    #89241

    Purpose

    Resurfaced Cell-penetrating GFP nanobody

    Depositor

    Brian McNaughton

    Article

    Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

    Insert

    pcNB1 (GFP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ASB9A-c009

    Plasmid

    #53670

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    ASB9A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only