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32,812 results

Showing: 6841 - 6870 of 32812 results

  4. pET28a Gx-NL

    Plasmid
    #169105
    Purpose
    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169105-plasmid-map-sequence-id-335658

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  5. pAC1430

    Plasmid
    #127981
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127981-plasmid-map-sequence-id-254494

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  14. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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  19. 4X3G

    Plasmid
    #64663
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIAH1 (SIAH1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    April 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. p406GALL

    Plasmid
    #17434
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALL promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17434-plasmid-map-sequence-id-8608

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  28. pJP_Osc05

    Plasmid
    #229040
    Purpose
    Light inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.
    Depositor
    Yolanda Schaerli
    Article
    Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038
    Insert
    pBAD promoter
    Expression
    Bacterial
    Available Since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229040-plasmid-map-sequence-id-458369

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Showing: 6841 - 6870 of 32812 results