30,803 results

1. MET-HYGRO

   Plasmid

   #218113

   Purpose

   Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LYS-Leu2

   Plasmid

   #218114

   Purpose

   Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

   Plasmid

   #60815

   Purpose

   dCas9 with single cysteine residue (M1C) for site-specific labelling

   Depositor

   Jennifer Doudna

   Article

   O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

   Insert

   dCas9 M1C D10A C80S H840A C574S
   

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   M1C D10A C80S H840A C574S

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGDP3 rmtB

   Plasmid

   #112880

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   rmtB (RmtB Escherichia coli)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRBV20-1

   Plasmid

   #123454

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV20-1 (TRBV20-1 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCerulean-pBAD

   Plasmid

   #54666

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Type

   Empty backbone

   Tags

   6xHis and mCerulean

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pTA-HUH

   Plasmid

   #221152

   Purpose

   HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

   Depositor

   Sheng Yang

   Article

   Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

   Insert

   HisG-URA3-HisG
   

   Expression

   Bacterial and Yeast

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad-UXPS

   Plasmid

   #172138

   Purpose

   bacteria expression plasmid for a UDP/UTP sensor

   Depositor

   Huiwang Ai

   Article

   Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

   Insert

   UXPS
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYCDuet-Myc-MBP-HA

   Plasmid

   #222711

   Purpose

   Expression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL-C2X dAmp/Kana+

    Plasmid

    #154352

    Purpose

    Empty backbone vector

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGDP4 ermC

    Plasmid

    #112884

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    ermC (ermM Staphylococcus epidermis)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEcoFus

    Plasmid

    #187833

    Purpose

    1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpression

    Depositor

    Jörg Vogel

    Article

    Ponath et al Nat Microbiol. 2021 Aug;6(8):1007-1020.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJH65

    Plasmid

    #67273

    Purpose

    Conjugally transferable red recombinogenic plasmid

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    tetA
    

    Use

    Red recombinase, temperature sensitive

    Expression

    Bacterial

    Promoter

    tetA promoter of plasmid pACYC184 origin

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMApra-Para

    Plasmid

    #218362

    Purpose

    Empty backbone with araC-ParaBAD expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBXNPHM3

    Plasmid

    #110099

    Purpose

    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GEF-H1 FLARE212.pa

    Plasmid

    #134930

    Purpose

    GEF-H1 biosensor that does not have GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-J23109

    Plasmid

    #113151

    Purpose

    Plasmid constitutively expressing dCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    dCas9
    

    Expression

    Bacterial

    Mutation

    D10A & H840A

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pR562

    Plasmid

    #207886

    Purpose

    Creation of Level 0 entry clones

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    amilCP
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC020 - LseCas13a from Listeria seeligeri

    Plasmid

    #91910

    Purpose

    Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LseCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T-1-3xHA

    Plasmid

    #129567

    Purpose

    Empty backbone for HA tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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