32,167 results
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Plasmid#173935PurposeBacterial expression of PARP1 lacking Zn2 domainDepositorInsertPARP1delZn2 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 97-213 (V762A)Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-weak
Plasmid#108314PurposeGFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterpFAB4282Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)- PC-MjMAT
Plasmid#179372PurposePC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.DepositorInsertPC-MjMAT
Tags6xHisExpressionBacterialMutationL147A, I351APromoterT7Available SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG2_Actin_2
Plasmid#109990PurposeProtein expression and purification of ACTG2_Actin_2DepositorInsertACTG2_Actin_2 (ACTG2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3
Plasmid#197472PurposeBacterial Expression of human ATG3DepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
QUAS-0-T7-GFP_ColE1
Plasmid#171649PurposeQUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-0-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF_p15A
Plasmid#171651PurposeExpresses QF activator protein in BL21(DE3) E. coli. Contains p15A origin of replication.DepositorInsertQF-T7 Stop
ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-GFP_ColE1
Plasmid#171653PurposeQUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-5-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-10-T7-GFP_ColE1
Plasmid#171654PurposeQUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-10-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-15-T7-GFP_ColE1
Plasmid#171655PurposeQUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-15-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcA
Plasmid#107876PurposeExpresses E. coli RtcA in bacterial expression cellsDepositorAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
ExpressionBacterialAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT006-GCN4-HaloPM
Plasmid#163386PurposeTo express 4X sGCN4 module in the G-baToN systemDepositorInsert4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME Integrin beta 1b
Plasmid#105976Purposeentry clone for gateway cloning, Full length Integrin beta 1b without stopDepositorInsertIntegrinbeta1b
ExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Kindlin
Plasmid#186135PurposeFor expression of recombinant kindlin in bacteriaDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF3
Plasmid#228777PurposeProtein expression plasmid for YTH domain of YTHDF3 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA(D34A/D169A)
Plasmid#108693PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon, …PromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_LC3A
Plasmid#73946PurposeFor recombinant expression of human LC3A/MAP1LC3A in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRExpressionBacterialMutationD485APromoterPJ23108Available SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-I
Plasmid#17634DepositorInsertExpressionBacterialAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJ2076.2_TS
Plasmid#140667PurposeToggle switch (TS)DepositorInsertToggle switch (TS)
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEZYHb
Plasmid#18669PurposepKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteriaDepositorTypeEmpty backboneTagshemoglobinExpressionBacterialAvailable SinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GAS7-1/1
Plasmid#91301PurposeProtein expression and purification of human SH3 domain construct GAS7-1/1DepositorInsertGAS7-1/1 (GAS7 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr
Plasmid#190794PurposeTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.DepositorInsertsgRNA-scaffold only
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
Tags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMMB66EH-aphA
Plasmid#44392DepositorInsertAphA
ExpressionBacterialPromotertacAvailable SinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMM627
Plasmid#112532PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
ExpressionBacterialPromoterJ23107, PL(HrtO), and ProDAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSII(KSplus)-HsBGG-WT_E
Plasmid#146187PurposeBacterial Expression of HsBGG-WTDepositorInsertHsBGG-WT
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
ExpressionBacterial and YeastAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only