32,812 results
-
Plasmid#172138Purposebacteria expression plasmid for a UDP/UTP sensorDepositorInsertUXPS
ExpressionBacterialPromoteraraBADAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; IExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Rin1-SH2
Plasmid#46474DepositorInsertRin1 (RIN1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 54-204PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFCiKm7
Plasmid#167521PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.DepositorInsertCinRAM-KmR
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2
Plasmid#99330PurposeMammalian expression of NRBF2, which associates with the PI3KC3-C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
TagsHis-MBPExpressionBacterialPromoterT7Available SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-PvanCC-mVenus_vanR
Plasmid#137664PurposeConjugative vector derived from pSHDY. Constitutive expression of the repressor vanR, as well as vanillate-inducible expression of mVenus.DepositorInsertPvanCC:mVenus
ExpressionBacterialPromoterPvanCCAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBL090J
Plasmid#195045PurposecCadC-CCB3.5 under the control of PBADDepositorInsertcCadC-CCB3.5
ExpressionBacterialAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos14)GFP
Plasmid#89252PurposeExpresses GFP with +14 theoretical charge in E.ColiDepositorInsertHis6-(pos16)-GFP
TagsHis6ExpressionBacterialPromoterT7Available SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP4
Plasmid#129507PurposeExpresses AvicFP4 constitutively in E. coli (most strains)DepositorInsertAvicFP4
ExpressionBacterialMutationOne of two variants of AvicFP4 tested with essent…Promotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PunyEEER
Plasmid#208322PurposeBacterial expression of the His-tagged peptide PunyEEER.DepositorInsertPunyERRE
TagsDBCO-Tag, His-TagExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-TinyERRE
Plasmid#208320PurposeBacterial expression of the His-tagged peptide TinyERRE.DepositorInsertTinyERRE
TagsDBCO-Tag, His-TagExpressionBacterialAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 2A-CVB3
Plasmid#202076PurposeBacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 2A protease with HisTag
Tags6xHis-tagExpressionBacterial, Insect, and Mamm…Promoterpolh, CAG, T7Available SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDx_mCherry-SYFP2
Plasmid#189759PurposeDual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysisDepositorInsertmCherry-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-BoNT/X
Plasmid#168017PurposeExpresses BoNT/X-LC(1-439)-6xHis in bacterial cellsDepositorInsertBoNT/X-LC(1-439)-6xHis
TagsHisx6ExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
APneg-SNAP25a
Plasmid#168011Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD18HMGR
Plasmid#19041DepositorInserttruncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaRlux
Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-luxCDABE-term
UseSynthetic BiologyExpressionBacterialPromoterPesaRAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP toehold switch
Plasmid#107355PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressedDepositorInserttoehold 7 + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q
Plasmid#84352PurposeExpression of the human Huntingtin Exon1 protein containing 43Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGMC00018 (aka pRC0082)
Plasmid#195320PurposeMinimal plasmid backbone with multiple cloning site for HDR donor DNA constructionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterial and MammalianAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX 6P1 Cyclin A (555)
Plasmid#61844Purposebacterial expression of GST cyclin ADepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI
Plasmid#117005PurposeExpresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24-1A(sfGFP)
Plasmid#126971PurposeLevel 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Gamma crystallin TEV LIC cloning vector (1X)
Plasmid#29661DepositorTypeEmpty backboneTags6xHis, Gamma-crystallin, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDule-Acd82
Plasmid#174099PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertAcridone 82 tRNA synthetase and tRNA
ExpressionBacterialMutationN311S, C313G, V366A, W382TAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLxR5
Plasmid#149465PurposeBroad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS Wnt-2HA
Plasmid#17368DepositorAvailable SinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
Tags6xhisExpressionBacterialPromoterT7 promoterAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC059-HisMS2_PLP_NucPhos_pac
Plasmid#155039PurposeGeneration of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein geneDepositorInsertsMaturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein
ExpressionBacterialMutationRemove TypeIIs Restriction SitesPromoterT7Available SinceSept. 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits