32,167 results

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pET22b_msfGFP_stag_KS(FtoG)

Plasmid

#237631

Purpose

For expression of msfGFP_stag_KS(FtoG) in Ecoli

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

stag_KS(FtoG)

Tags

msfGFP

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate PRVABC59 NS3 helicase domain

Plasmid

#228664

Purpose

Bacterial expression of the Zika virus NS3 helicase

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika virus NS3 helicase

Tags

His-GST-TEV

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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RoL10X_pET29b

Plasmid

#215427

Purpose

Expression construct for RoL10X gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

RoL10X

Expression

Bacterial

Mutation

V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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ThcCut1_pET29b

Plasmid

#215429

Purpose

Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ThcCut1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJOE4847.2

Plasmid

#135086

Purpose

rhamnose-inducible expression vector

Depositor

Josef Altenbuchner

Article

Motejadded et al Biotechnol Lett. 2009 Apr;31(4):543-9. d

Insert

MalE-6xHis-Ulp1

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa2-A69T

Plasmid

#98578

Purpose

photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

pcDronpa2-A69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGST-Vps4

Plasmid

#21495

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS4 (VPS4 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAC575

Plasmid

#127976

Purpose

AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

ble (bleomycin resistance marker)

Use

Fungal expression

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPhlF-P3

Plasmid

#74687

Purpose

PhlF-P3 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSP3-PhlF-PPhlF-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShoHELIX-KanR (BO4)

Plasmid

#181795

Purpose

pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCPP3416

Plasmid

#128720

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopT1-1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5534

Plasmid

#128723

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopX1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLicC-MBP-APETx2

Plasmid

#72668

Purpose

Periplasmic expression of APETx2 in E.coli

Depositor

Glenn King

Article

Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

Insert

APETx2

Tags

His tag, MBP, MalE signal sequence for periplasmi…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pHYRSF1-02

Plasmid

#24765

Depositor

Hideo Iwai

Article

Muona et al Nat Protoc. 2010 . 5(3):574-87.

Insert

NpuDnaE-IntC

Tags

GB1 and His

Expression

Bacterial

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-12xGCN4

Plasmid

#205158

Purpose

express tdMCP-12xGCN4

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-12xGCN4

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b/EYFP-GgVcl 884-1066-ECFP FRET

Plasmid

#46179

Depositor

Susan Craig

Insert

Vcl 884-1066 (VCL Chicken)

Tags

ECFP, EYFP, and His

Expression

Bacterial

Mutation

aa 884-1066 (Vt, tail domain)

Promoter

T7

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pET mCerulean LIC cloning vector (u-mCerulean)

Plasmid

#29773

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-BsaI_pJMP2748

Plasmid

#208888

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Use

Pir dependent origin of replication

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEA01

Plasmid

#170766

Purpose

Utilisation of pSAM2 excision tool in Amycolatopsis spp

Depositor

Jean-Luc Pernodet

Article

Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

Inserts

xis, int
oriT
pac

Expression

Bacterial

Promoter

trcp

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAV10.F3

Plasmid

#63199

Purpose

BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/pLas eGFP

Plasmid

#193630

Purpose

Inducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNAP promoter

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRAU168

Plasmid

#86834

Purpose

bacterial expression of anti-CRISPR protein AcrIIA1

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA1

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pPBC-G6f

Plasmid

#62811

Purpose

pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6f-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pWBC

Plasmid

#15239

Depositor

Ron Vale

Article

Niclas et al Neuron. 1994 May . 12(5):1059-72.

Insert

Human neuronal kinesin heavy chain (KIF5A Human)

Expression

Bacterial

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pClodANL-FGF4

Plasmid

#107874

Purpose

Bacterial expression of FGF4-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF4 (FGF4 Synthetic)

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-cTF

Plasmid

#87743

Purpose

IPTG-inducible expression of ligase-cTF fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and cTF (chimeric transcription factor base…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJF093

Plasmid

#113323

Purpose

Sp.pCas9-dCas9, pTet-MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-plu-gamma-ET_rhi145

Plasmid

#180427

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ET_rhi145

Expression

Bacterial

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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P70a-deGFP-sc101-kan

Plasmid

#184841

Purpose

Expression of deGFP

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

promoter OR2-OR1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBS Olfactomedin1

Plasmid

#19225

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Olfactomedin 1 (OLFM1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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