31,059 results
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Plasmid#178484PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only
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pSZT025
Plasmid#140033PurposeKO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
ExpressionBacterialPromotercLac143Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
HSP90B1
Plasmid#39076PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds
Plasmid#119059PurposeFluorescent vector with zebrafish pax3a enhancer 5 (strong activity)DepositorInsertpax3a_enh5-E1b:eGFP
ExpressionBacterialPromoterE1b minimal promoterAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAK463
Plasmid#140701PurposeNBαMs-IgKDepositorInsertNBαMs-IgK
ExpressionBacterialAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJS010
Plasmid#188333PurposePCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mKate2::mIAA7
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJS004
Plasmid#188327PurposePCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mCherry::mIAA7
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL004
Plasmid#180608PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertionDepositorInsertPlmCasX with DpbCasX R1 loop
UseCRISPRTags10xHis and MBPExpressionBacterialPromoterT7Available SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK633
Plasmid#72397PurposeProduces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)DepositorInsertphosphoribosylaminoimidazole synthetase
TagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK515
Plasmid#72385PurposeProduces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)DepositorInsertphosphoribosylglycinamide formyltransferase 2
TagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX CnA 2-347
Plasmid#13251DepositorInsertCnA (PPP3CA Human)
TagsGSTExpressionBacterialMutationAmino acids 2-347 (truncated before B binding hel…Available SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL + STS
Plasmid#139790Purposeexpression of biosynthetic enzymes 4CL and STSDepositorInserts4CL
STS
ExpressionBacterialAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
AD-003
Plasmid#25502PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBJG1 T7-8xHis-OGT 5N5A
Plasmid#154280PurposeBacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationN322A, N356A, N390A, N424A, N458A mutations; bact…Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2
Plasmid#109289PurposeExpresses high levels of protein in mycobacteriaDepositorTypeEmpty backboneExpressionBacterialMutationpSUM-kan-MCS1-gfp was modified to reorient the Ba…Available SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEN1-SGC
Plasmid#39127PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTagsStrep tag and SET1ExpressionBacterialAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJ2044
Plasmid#140676PurposeNOT gate with sg-1DepositorInsertNOT gate with sg-1
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Intersectin I SH3 A domain (human)
Plasmid#47413DepositorInsertIntersectin SH3 domain A (ITSN1 Human)
TagsGSTExpressionBacterialMutationonly SH3 domain APromotertacAvailable SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATOH1_pUC18
Plasmid#191250PurposeEncodes the sequence for the ATOH1 gene (human)DepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-mRFP
Plasmid#113553PurposeFor bacterial expression and purification of mRFPDepositorInserthis-mRFP
ExpressionBacterialAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S222TAG
Plasmid#68302PurposeN-terminal MBP tagged human MEK1 gene S222TAG for bacterial expressionDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SNX5-PX (26-170)
Plasmid#119086PurposeBacterial expression of human phox homology (PX) domain, SNX5-PX (26-170)DepositorAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pReporter_2
Plasmid#60563PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_2
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Lyn-SH2
Plasmid#46452DepositorInsertLyn (LYN Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 106-213PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXC9 (murine) HIS-tag pET
Plasmid#8559DepositorAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-GAL4DBD
Plasmid#26256DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisExpressionBacterial and InsectAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-HpENR
Plasmid#12651DepositorInsertnoyl-acyl carrier protein reductase (fabI Helicobacter pylori)
ExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
B6-2
Plasmid#111486PurposePro-tRNA synthetaseDepositorInsertPro-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Col1A-5'probe
Plasmid#20732DepositorInsertCol1A-5'probe (Col1a1 Mouse)
ExpressionBacterialAvailable SinceApril 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
HARS_pNIC-Bio3
Plasmid#153050PurposeExpression construct for producing Avi tagged histidyl tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
GMPSA
Plasmid#42402PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBLO5.01
Plasmid#121418PurposepUC19_BsaI-dCas9-BsaIDepositorInsertdcas9
ExpressionBacterialPromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFPcR5
Plasmid#149484PurposeBroad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertPcaU(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterP3B5BAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
LB1366 (Bcl-2 promoter with MHS1234)
Plasmid#15388DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS1234Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEE077
Plasmid#176834PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert716
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRF-ScbR
Plasmid#49370DepositorInsertScbR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Ptrc-base
Plasmid#169243PurposeSuicide vector for integrating expression cassette in P. putida (IPTG induction)DepositorInsertlacI
ExpressionBacterialPromoterlacIqAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only