32,826 results
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Plasmid#113397PurposeTy3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducibleDepositorInsertTy3 Gag3 codon optimized for E. coli expression
TagsGag3-6XHis tagExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.719
Plasmid#108520PurposePTtg-YFP Reporter. Nar sensor for use in Marionette strain.DepositorInsertPTtg-YFP
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.711
Plasmid#108512PurposePPhlF-YFP Reporter. DAPG sensor for use in Marionette strain.DepositorInsertPPhlF-YFP
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK563
Plasmid#110612PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoterDepositorInsertNanoluc
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Endophilin II Full
Plasmid#47409DepositorInsertEndophilin II
TagsGSTExpressionBacterialPromotertacAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW209
Plasmid#113188PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR arrayDepositorInsertsCas1
Cas2
Leader-repeat
ExpressionBacterialAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_314
Plasmid#111822PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 294-314
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Integrin beta 1b-mKate
Plasmid#105975Purposein vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.DepositorInsertIntegrinbeta1b
TagsmKateExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her2-dL5-Her2
Plasmid#85624PurposeBacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Human, Synthetic, Schistosoma japonicum)
Tags10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3β ctrl
Plasmid#112031PurposeMode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)DepositorInsert14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
TagsSplit mCherryExpressionBacterialAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
B2-3
Plasmid#111470PurposeLys-tRNA synthetaseDepositorInsertLys-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.gfp
Plasmid#112516PurposeProB driven gfpmut3 expression with synTTDepositorInsertProB gfpmut3 TT
ExpressionBacterialPromoterProBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2
Plasmid#111269Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
ExpressionBacterialPromoterT7Available SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB410 (His-Ub-Ate1-4 1A7B)
Plasmid#104339PurposeExpress His Tag Ate1-4 1A7BDepositorAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC.Taq.1Amb
Plasmid#102794PurposeExpresses Taq DNA polymerases with a premature stop codonDepositorInsertTaq DNA polymerase
ExpressionBacterialMutationW168Stop; *(see below)Available SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUWL201PW-creDE
Plasmid#112241PurposeStreptomyces/E.coli shuttle expression vector encoding creD and creEDepositorInsertscreD
creE
ExpressionBacterialAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBY2_ligD(FnCpf1)
Plasmid#104623PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligDDepositorInsertcrRNA to ligD
UseCRISPRExpressionBacterialPromoterPj23119Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2
Plasmid#111086PurposeFor production of recombinant siRNAs (pro-siRNAs) in E. coli.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSum-LacZ-gfp
Plasmid#109285PurposeExpresses gfp in mycobacteriaDepositorInsertGFP
ExpressionBacterialMutationGFP modified for expression in prokaryotes; inclu…PromoterLacZalphaAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B2-2
Plasmid#111469PurposeThr-tRNA synthetaseDepositorInsertThr-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-2
Plasmid#111476PurposeAsp-tRNA synthetaseDepositorInsertAsp-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-3
Plasmid#111482PurposeCys-tRNA synthetaseDepositorInsertCys-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-3
Plasmid#111495PurposeHis-tRNA synthetaseDepositorInsertHis-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-1
Plasmid#111494PurposeGly-tRNA synthetase BDepositorInsertGly-tRNA synthetase B
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-4
Plasmid#111496PurposeGly-tRNA synthetase ADepositorInsertGly-tRNA synthetase A
ExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-3
Plasmid#111477PurposeAsn-tRNA synthetaseDepositorInsertAsn-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human RNF144B (27-236)
Plasmid#110753PurposeBacterial Expression of RNF144B RBR domainDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_E83K
Plasmid#109412PurposeRepA MutantDepositorInsertrepA
TagsRFPExpressionBacterialMutationE83K repA mutation to increase copy numberAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only