30,307 results

1. pAB50

   Plasmid

   #101678

   Purpose

   mCherry under T7 promoter control

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   mCherry under T7 promoter control
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDsEndoNA2

   Plasmid

   #174728

   Purpose

   Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

   Insert

   DsRed-Monomer
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCB2-sgRNA

   Plasmid

   #129463

   Purpose

   Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   pgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 (SpeI)

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. human MTAP

   Plasmid

   #64077

   Purpose

   Expression of human 5'-methylthioadenosine phosphorylase

   Depositor

   Vern Schramm

   Article

   Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

   Insert

   5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)
   

   Use

   Tags

   Histidine tag and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Butelase AY

   Plasmid

   #221847

   Purpose

   Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

   Insert

   Butelase 1 ligase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Val237Ala, Thr238Tyr

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLxVi5

   Plasmid

   #167516

   Purpose

   Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-Violacein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-NM23-H1

   Plasmid

   #157987

   Purpose

   Expresses NM23-H1 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Han et al Bulletin of the Kor Chem Soc 2010; 31(5)

   Insert

   NM23-H1 (NME1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBADHISTEV_PfeE

   Plasmid

   #129120

   Purpose

   pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   PBAD

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a T7pCONS sfGFP

   Plasmid

   #154462

   Purpose

   pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-6P-1-HsRCK_E

    Plasmid

    #146210

    Purpose

    Bacterial Expression of HsRck

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsRck (DDX6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a(+)::MBP-NL3F10H

    Plasmid

    #141291

    Purpose

    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    MBP-NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3), His (x10), and MBP

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-Dnmt3a3L-sc27

    Plasmid

    #71827

    Purpose

    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

    Depositor

    Albert Jeltsch

    Article

    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

    Insert

    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p7XNH3

    Plasmid

    #47064

    Purpose

    FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMUT2-pLac-mCherry

    Plasmid

    #192859

    Purpose

    pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    lldR operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET19b+H2B(T116C)

    Plasmid

    #157808

    Purpose

    Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H2B(T116C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T116C

    Promoter

    T7

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. B4-4

    Plasmid

    #111478

    Purpose

    translational release factor 1

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    translational release factor 1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFPV25-cR-2iG

    Plasmid

    #204618

    Purpose

    Fluorescent reporter for SPI2 induction in Salmonella

    Depositor

    Jacques Neefjes

    Article

    Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

    Inserts

    rpsM promoter
    sseA promoter
    

    Use

    Tags

    GFP and dsRed

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRAF-MutL

    Plasmid

    #86607

    Purpose

    E. coli MutL expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutL
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEM-3z/602

    Plasmid

    #26657

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only