30,808 results

1. pcDNA-tdMCP-24xGCN4

   Plasmid

   #205157

   Purpose

   express tdMCP-24xGCN4

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   tdMCP-24xGCN4
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS Zpapc

   Plasmid

   #25774

   Depositor

   Edward De Robertis

   Article

   Yamamoto et al Development. 1998 Sep . 125(17):3389-97.

   Insert

   Papc (pcdh8 Zebrafish)
   

   Expression

   Bacterial

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

3. AcrIIA4

   Plasmid

   #101043

   Purpose

   Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

   Depositor

   Zhiwei Huang

   Article

   Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

   Insert

   AcrIIA4
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTPH413

   Plasmid

   #185728

   Purpose

   Bacterial PAM profiling of adenine base editor variants

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Inserts

   ecTadA(8e)-neNme2-C
   Nme2Cas9 sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Otx1/pBS

   Plasmid

   #16332

   Depositor

   Peter Klein

   Insert

   Otx1 (otx1 Frog)
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCAT220

   Plasmid

   #134888

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAR100

   Plasmid

   #180377

   Purpose

   pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).

   Depositor

   Alec Redwood

   Article

   Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:

   Inserts

   NFAT-Luciferase
   hCD8A
   

   Expression

   Bacterial and Mammalian

   Promoter

   hEF1-HTLV and minimal promoter and an NFAT respon…

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a - NR

   Plasmid

   #89625

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Nitrate reductase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. YWHAE

   Plasmid

   #39146

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   YWHAE (YWHAE Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. BRD3

    Plasmid

    #38941

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD3 (BRD3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-SARS-CoV-2 NSP13

    Plasmid

    #176492

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

    Depositor

    Xiaodong Cheng

    Article

    White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

    Insert

    SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
    

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    I570 from SARS-CoV NSP13 was mutated to V to conv…

    Promoter

    T7

    Available Since

    Jan. 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJL1 eforRed

    Plasmid

    #106320

    Purpose

    Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    eforRed
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY003 (pFnCpf1_delta Cas)

    Plasmid

    #69974

    Purpose

    Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus without Cas genes
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Cas genes are deleted from locus

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-Pak1 83-149

    Plasmid

    #12216

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Pak1 (Pak1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Dominant negative Pak1: autoinhibitory domain, aa…

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15-His-∆NGlpG

    Plasmid

    #155131

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115270

    Purpose

    Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    TtCsm6
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJBM3

    Plasmid

    #225290

    Purpose

    E. coli-Campylobacter shuttle vector; KanR

    Depositor

    Robert Moore

    Article

    McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

    Insert

    Replication elements from pCC388 for pJBM3
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbS5c-Maqu_3494

    Plasmid

    #86205

    Purpose

    Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

    Depositor

    Mary Dunlop

    Article

    Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

    Insert

    Efflux pump
    

    Expression

    Bacterial

    Promoter

    ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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