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31,059 results

Showing: 6201 - 6220 of 31059 results

  4. pExp-Bla

    Plasmid
    #112561
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-Bla and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112561-plasmid-map-sequence-id-217226

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  5. pDT386

    Plasmid
    #174733
    Purpose
    Expresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    DT386
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Deleted amino acids 412-560 (the receptor binding…
    Promoter
    T5
    Available Since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174733-plasmid-map-sequence-id-343525

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  6. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available Since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  7. pPEPZ-sgRNAclone

    Plasmid
    #141090
    Purpose
    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107
    Insert
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    p3
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    141090-plasmid-map-sequence-id-330475

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  14. pNtCp-rbcL-PHLS-GPPS-aadA-accD

    Plasmid
    #52471
    Purpose
    Inserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-GPPS-aadA-accD
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52471-plasmid-map-sequence-id-81334

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  15. pET21b-MBPx-MBPx-SpyTag003

    Plasmid
    #216291
    Purpose
    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MBPx-MBPx-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 and SpyTag003
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216291-plasmid-map-sequence-id-443903

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  20. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Tags
    4FLAG
    Expression
    Bacterial
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

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Showing: 6201 - 6220 of 31059 results