33,123 results

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BbZIP-cALFA

Plasmid

#160498

Purpose

For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

BbZIP

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Bacterial

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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6NM4

Plasmid

#162257

Purpose

Bacterial Expression

Depositor

Cheryl Arrowsmith

Insert

PRDM9 (PRDM9 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2_N_deltalinker

Plasmid

#157870

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the linker

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaliner

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaCTD

Plasmid

#157871

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaCTD

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-AtC60αβC20NtL

Plasmid

#160876

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: T7, chaperones: T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2846

Plasmid

#160676

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ILF2_DZF_pGEX

Plasmid

#135162

Purpose

Recombinant expression of ILF2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

ILF2 (ILF2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-Tn5-F

Plasmid

#160081

Purpose

Bacterial expression of C-terminally FLAG-tagged Tn5 transposase

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_HSPA9_HSP70

Plasmid

#109776

Purpose

Protein expression and purification of HSPA9_HSP70

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HSPA9_HSP70 (HSPA9 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pKIKan_idCas9_Chr_center

Plasmid

#149637

Purpose

suicide vector for knock in into the B. burgdorferi chromosome

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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T7 promoter + filler DNA reporter plasmid

Plasmid

#156454

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pQLink-GST-10xCTD

Plasmid

#138472

Purpose

Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Tags

GST

Expression

Bacterial

Mutation

10x CTD repeat

Promoter

T5

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBsacA

Plasmid

#158650

Purpose

Constitutive transcription of sacA crRNA

Depositor

Wenjing Cui

Insert

sacA crRNA

Use

CRISPR and Synthetic Biology; Shuttle vector baci…

Expression

Bacterial

Promoter

Pveg

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoAp-GFPuv

Plasmid

#139072

Purpose

Low phosphate inducible promoter phoAp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoAp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-phzAG

Plasmid

#141106

Purpose

Contains a seven gene phzABCDEFG operon BioBrick

Depositor

Martin Buck

Article

Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

Insert

phzABCDEFG

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMC059-HisMS2_PLP_NucPhos_pac

Plasmid

#155039

Purpose

Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein gene

Depositor

Paul Freemont

Article

Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

Inserts

Maturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein

Expression

Bacterial

Mutation

Remove TypeIIs Restriction Sites

Promoter

T7

Available Since

Sept. 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJF143.J3

Plasmid

#153028

Purpose

J3-mRFP1

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

J3-mRFP1

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AT110.i1

Plasmid

#135481

Purpose

E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

Depositor

Andrew Kruse

Article

Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

Insert

Nanobody AT110.i1

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp7

Plasmid

#156476

Purpose

Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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