30,808 results

1. dKatushka-pBAD

   Plasmid

   #54775

   Purpose

   Localization: Protein Expression Vector , Excitation: 588, Emission: 635

   Depositor

   Dmitriy Chudakov, Michael Davidson

   Article

   Shcherbo et al Nat Methods. 2007 Sep . 4(9):741-6.

   Type

   Empty backbone

   Tags

   6xHis and dKatushka

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pVRa41_491

   Plasmid

   #49702

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF41_491
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbB19c-GFP

   Plasmid

   #126139

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30-T7-His-sfGFP

   Plasmid

   #180755

   Purpose

   pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Tags

   strep tag

   Expression

   Bacterial

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b/EYFP-GgVcl 884-1066-ECFP FRET

   Plasmid

   #46179

   Depositor

   Susan Craig

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Tags

   ECFP, EYFP, and His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (Vt, tail domain)

   Promoter

   T7

   Available Since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-(SUMO)1

   Plasmid

   #127089

   Purpose

   Bacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   EGFP human SUMO3 (SUMO3 Human)
   

   Tags

   EGFP

   Expression

   Bacterial

   Promoter

   unknown

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SUMO-AsCas12f

   Plasmid

   #171612

   Purpose

   AsCas12f1 heterologous expression plasmid

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

   Insert

   AsCas12f1
   

   Expression

   Bacterial

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMBEC8

   Plasmid

   #187599

   Purpose

   Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   nCas9-BEC-UGI; cas6f, gfp
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPTG-Tau-RING

   Plasmid

   #233691

   Purpose

   Expression of Tau-RING protein in bacteria

   Depositor

   Leo James

   Article

   Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

   Insert

   pOPTG
   

   Expression

   Bacterial

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcrVIB1

    Plasmid

    #184566

    Purpose

    Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBR4pemIK-GW

    Plasmid

    #126929

    Purpose

    Broad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selection

    Depositor

    Lindsey Burbank

    Article

    Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ1955

    Plasmid

    #84830

    Purpose

    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized tdTomato
    

    Expression

    Bacterial and Worm

    Promoter

    Peft-3

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLC-255

    Plasmid

    #124292

    Purpose

    Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

    Depositor

    Swaine Chen

    Article

    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

    Insert

    vsfGFP-9
    

    Expression

    Bacterial

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPerimCh

    Plasmid

    #227013

    Purpose

    Expresses periplasmic mCherry

    Depositor

    Suzan Rooijakkers

    Article

    Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

    Insert

    PelB-mCherry, arabinose inducible cytosolic GFP
    

    Expression

    Bacterial

    Promoter

    rpsM and pBAD

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSA2

    Plasmid

    #52262

    Purpose

    Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-2 (SUMO2 Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. HS-Ras-mKate2

    Plasmid

    #105951

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Ras
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. FH

    Plasmid

    #38978

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FH (FH Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pJMP2834

    Plasmid

    #160673

    Purpose

    Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Insert

    gmc6 (gfp) sgRNA
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MacroH2A1.1-c002

    Plasmid

    #173087

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A1 (MACROH2A1 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Q162-N369, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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