33,128 results
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Plasmid#74066PurposepAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecRDepositorInsertsgRNA pgi (T)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterptacAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reb_81 aka PAS622
Plasmid#71227PurposeReb_1 with RebB(T28A, D72G)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebB(T28A, D72G), RebA(G53A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Osh2-PH
Plasmid#71370Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertOsh2-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 277-398PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/spoR
Plasmid#73631PurposeProduces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tagDepositorInsertguanylate kinase
TagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a-AlkB deltaN11
Plasmid#73622PurposeExpresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tagDepositorInsertAlkB (alkB E. coli)
TagsHisExpressionBacterialMutationdeletion of first 11 AAPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO HygroR
Plasmid#68445PurposeTOPO construct expressing HygroR for generation of GMAP-compatible geneDepositorInsertHygromycin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO ZeoR
Plasmid#68446PurposeTOPO construct expressing ZeoR for generation of GMAP-compatible geneDepositorInsertZeocin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO PuroR
Plasmid#68447PurposeTOPO construct expressing PuroR for generation of GMAP-compatible geneDepositorInsertPuromycin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO 2-5
Plasmid#68408PurposeTOPO backbone for GMAP genes with sites #2 and #5DepositorTypeEmpty backboneUseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-mTmod3-WT
Plasmid#70753Purposemouse Tropomodulin3, WT for bacterial expressionDepositorAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-E138A
Plasmid#69921PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsFLAGExpressionBacterial and MammalianMutationE138APromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL542
Plasmid#70282PurposeColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TRP120
Plasmid#71732Purposeinducible expression of TRP120 in E. coliDepositorInsertTRP120
Tags6xHIS and ThioredoxinExpressionBacterialPromoterpBADAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP5
Plasmid#71468PurposeEncodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 5 (SENP5 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hp MTAN
Plasmid#69802PurposeExpression of H. pylori J99 methylthioadenosine nucleosidaseDepositorInsertMethylthioadenosine nucleosidase
TagsHistidine tagExpressionBacterialAvailable SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
NM-01
Plasmid#69799PurposeBacterial expression of Cas9 with His, MBP, and Strep tagsDepositorInsertCas9
TagsHIS, MBP, and StrepIIExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …Available SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-UPRT
Plasmid#69310Purposeexpresses UPRT cDNADepositorInsertUPRT cDNA
TagsHAExpressionBacterialAvailable SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD-rxRFP1.2
Plasmid#67839Purposeredox-sensitive red fluorescent protein rxRFP1.2DepositorInsertrxRFP1.2
TagsHis TagExpressionBacterialPromoterpBADAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF6-V5-His6
Plasmid#67415PurposeExpression of hArf6 with C-term V5 and His6 tag in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLpp5oGFP
Plasmid#69345PurposeOrthogonal GFP message under lpp5 promoterDepositorInsertsuper folder GFP
ExpressionBacterialAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only