32,838 results
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Plasmid#53208PurposeExpressed N-terminally Hexahistine tagged TRF2 Delta Basic proteinDepositorInsertTRF2 Delta Basic (TERF2 Human)
Tags6x HisExpressionBacterialMutationcontains amino acids 47-500PromoterTrcAvailable SinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
BLMA-c800
Plasmid#53631Purposebacterial expression plasmidDepositorInsertBLMA
TagsHis-TevExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 High pI protein 85 TEV LIC cloning vector (2-HpI-85)
Plasmid#55213DepositorTypeEmpty backboneTags6xHis, High pI protein 85, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL030
Plasmid#53533Purposesupplies EtpC required for optimal EtpA expression on compatible plasmidDepositorInsertetpC
UsePacyc184ExpressionBacterialAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(cAMP-3.3)/pRSETB
Plasmid#53592PurposeNano-lantern-based luminescent cAMP indicatorDepositorInsertNano-lantern-based luminescent cAMP indicator
Tags6xHisExpressionBacterialAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
ETV1A-c014
Plasmid#53655Purposebacterial expression plasmidDepositorInsertETV1A
ExpressionBacterialMutationBacterial expression for structure determination;…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
ASB9A-c009
Plasmid#53670Purposebacterial expression plasmidDepositorInsertASB9A
TagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B DN
Plasmid#49529Purposecloning vector for dominant negative chick BMP receptor 1BDepositorInsertBMPR-1B DN (BMPR1B Chicken)
ExpressionBacterialMutationdominant negative mutation (K231R); also P2A, M11…PromoterT3Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 651
Plasmid#53016PurposeLacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).DepositorInsertsLacIq
tetR
UseSynthetic BiologyTagsRBS(st3) and T1 terminatorExpressionBacterialPromoterpConAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1454
Plasmid#53019PurposeAmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertfimB
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlac0-1Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1277
Plasmid#53017PurposeAmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1DepositorInsertsGFP
mCherry
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR4-Dact1(1250-1601)
Plasmid#52046PurposeTo generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.DepositorInsertDact1(1250-1601) (Dact1 Mouse)
ExpressionBacterialAvailable SinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-hMeCP2-R270X
Plasmid#48092PurposeBacterial expression of Mxe intein/chitin binding domain tagged MeCP2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
ExpressionBacterialMutationMxe intein/chitin binding domain tagged, expresse…Available SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
psbA2(noATbox)-PHLS (c)
Plasmid#52308PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyExpressionBacterialMutationCAAATACA box in the psbA2 promoter to ggcgcgccPromoterpsbA2Available SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClbN-NHis
Plasmid#51497PurposeNRPS module containing CATE domains with N-terminal 6His tagDepositorInsertClbN
Tags6x His tag and T7 tagExpressionBacterialMutationcontains aa30-1094 of NP_754347.1PromoterT7Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
CT-Nano-lantern(ATP1)/pRSETB
Plasmid#51990Purposebacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplastsDepositorInsertNano-lantern_ATP1
Tags6xHis and chloroplast transit peptidExpressionBacterialMutationS257G in Rluc8Available SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(ATP1)/pRSETB
Plasmid#51988Purposebacterial expression of a Nano-lantern-based luminescent ATP indicatorDepositorInsertNano-lantern_ATP1
Tags6xHisExpressionBacterialMutationS257G in Rluc8Available SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbP
Plasmid#48244PurposeUntagged, full-length protein for in vivo peptidase assaysDepositorInsertClbP
TagsNoneExpressionBacterialPromoterT7Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbPpep-CHis
Plasmid#48243PurposeFirst 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domainDepositorInsertClbP-pep
Tags6-HisExpressionBacterialMutationFirst 375 amino acids of the peptidase, which con…PromoterT7Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-deltaPYD
Plasmid#51522PurposeExpress His6-tagged HIN domain of hAIM2 in bacteriaDepositorInsertAIM2 (AIM2 Human)
TagsHis6 and T7ExpressionBacterialMutationdeletion of pyrin domainPromoterlac operatorAvailable SinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQYD
Plasmid#48104PurposeDestination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion proteinDepositorTypeEmpty backboneTagsSYFP2ExpressionBacterialPromoterPQ5Available SinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQMD
Plasmid#48103PurposeDestination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion proteinDepositorTypeEmpty backboneTagsmyc tagExpressionBacterialPromoterPQ5Available SinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQHD
Plasmid#48102PurposeDestination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion proteinDepositorTypeEmpty backboneTagsHA tagExpressionBacterialPromoterPQ5Available SinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C3
Plasmid#50355PurposeMouse eIF2C3 cDNA cloneDepositorAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C1
Plasmid#50353PurposeMouse eIF2C1 cDNA cloneDepositorAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRF-HapR
Plasmid#49360DepositorInsertHapR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRF-SmcR
Plasmid#49371DepositorInsertSmcR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pVRa32_1122
Plasmid#49689DepositorInsertECF32_1122
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only