32,218 results

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pMR150

Plasmid

#32292

Depositor

Verne Chapman, Rudolf Jaenisch

Article

Sanford et al Genes Dev. 1987 Dec;1(10):1039-46.

Insert

genomic methylation probe-minor satellite repeat

Expression

Bacterial

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
MSP578

Plasmid

#98808

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-HNLYFQS-GST

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSP808

Plasmid

#98812

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-HNLYGHS-GST

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET30-hDlg PDZ2

Plasmid

#21692

Depositor

James Baleja

Article

Liu et al Biochemistry. 2007 Sep 25. 46(38):10864-

Insert

hDlg/SAP97 PDZ2 domain (DLG1 Human)

Tags

His6

Expression

Bacterial

Mutation

human hDlg/SAP97 PDZ2 domain (residues 318-405)

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pNIC-TolB-NHSTIIT-GG

Plasmid

#215792

Purpose

Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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C1-84 pComb3xss anti-cFos nanobody

Plasmid

#145830

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-84 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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ALFY-FYVE 1G

Plasmid

#197045

Purpose

Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

Insert

ALFY (WDFY3 Human)

Tags

6xHis-GST-TEV

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

Plasmid

#191635

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadR)-DadR

Plasmid

#191636

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ZFYVE9C-c009

Plasmid

#53618

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

ZFYVE9C

Tags

His-6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
VGFR1_HUMAN_D0

Plasmid

#79743

Purpose

This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VGFR1

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-EPSIN 1

Plasmid

#22225

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Tags

Xpress

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-WT

Plasmid

#29350

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
2P0E

Plasmid

#60109

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRSETB-S28 histone 3 phosphorylation reporter

Plasmid

#32788

Depositor

Alice Ting

Article

Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

Insert

CFP-14-3-3tau-H3 peptide-YFP

Tags

CFP and YFP

Expression

Bacterial

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only
AP582-2

Plasmid

#70049

Purpose

Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

mCherry::linker::H2B

Tags

Ollas and V5

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBM19-sd4U

Plasmid

#179692

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE4-DddA-G1397-C (T1413I)

Expression

Bacterial

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pStrepIHFb

Plasmid

#149385

Purpose

Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta

Tags

HRV3C cleavage site and StrepII-tag with

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103 FNBP3 WW domain #1

Plasmid

#104233

Purpose

Bacterial expression of WW domain #1 from FNBP3

Depositor

Sachdev Sidhu, Marius Sudol

Insert

FNBP3 WW domain #1 (PRPF40A Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available Since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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pED14xS-11

Plasmid

#134803

Purpose

IPTG inducible expression of S-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAT892

Plasmid

#68933

Purpose

Int9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Int9

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-mRFP

Plasmid

#27058

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-mcherry rfp

Expression

Bacterial

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pET28CLY6

Plasmid

#21765

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
inhibin-DsRed

Plasmid

#156184

Purpose

Expression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.

Depositor

Goro Yoshizaki

Article

Iwasaki-Takahashi et al Commun Biol. 2020 Jun 15;3(1):308. doi:

Inserts

rainbow trout inhibin α promoter
rainbow trout β actin 3'UTR

Expression

Bacterial

Promoter

inhibin

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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tISR

Plasmid

#122664

Purpose

Express tISR protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tISR (OCLM Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET29b-ClbPpep-CHis

Plasmid

#48243

Purpose

First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP-pep

Tags

6-His

Expression

Bacterial

Mutation

First 375 amino acids of the peptidase, which con…

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pETM6-M.accABCD

Plasmid

#66865

Purpose

Encodes accA, accB, accC, accD in monocistronic configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization Control

Plasmid

#186553

Purpose

Negative Control mediating endosomal localization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-EGFP

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-soNadV

Plasmid

#83363

Purpose

Expresses nadV from Shewanella oneidensis in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

Insert

nadV

Expression

Bacterial

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1-plus

Plasmid

#126775

Purpose

Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A; Q695A; Q926A; amino acids 1005-1013 replac…

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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