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31,059 results

Showing: 2051 - 2100 of 31059 results

  10. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  12. RAB27B

    Plasmid
    #25545
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB27B (RAB27B Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pCT02

    Plasmid
    #212152
    Purpose
    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
    Depositor
    Kang Zhou
    Insert
    FAST-PETase
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S121E, D186H, R224Q, N233K, R280A
    Promoter
    PT7
    Available Since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212152-plasmid-map-sequence-id-424774

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  30. proinsulin-EYFP

    Plasmid
    #218952
    Purpose
    Expresses proinsulin fused to EYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-EYFP
    Tags
    proinsulin
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218952-plasmid-map-sequence-id-446160

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  31. WT FL USP7

    Plasmid
    #227214
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227214-plasmid-map-sequence-id-452331

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  38. pmr101A-cPr-mCherry (ErmR)

    Plasmid
    #177206
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177206-plasmid-map-sequence-id-348606

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  39. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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  40. pSLTS

    Plasmid
    #59386
    Purpose
    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Type
    Empty backbone
    Use
    Genome engineering
    Expression
    Bacterial
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59386-plasmid-map-sequence-id-380750

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  43. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  44. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  45. SPLICSFAST-LY-MT-P2A

    Plasmid
    #214897
    Purpose
    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LY-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214897-plasmid-map-sequence-id-423483

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  48. pCgCas9_recT

    Plasmid
    #153337
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153337-plasmid-map-sequence-id-305977

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Showing: 2051 - 2100 of 31059 results