31,059 results
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Plasmid#102614PurposeExpression of S. cerevisiea RPA in E.coliDepositorExpressionBacterialMutationRFA2 has intron deletedPromoterT7Available SinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only
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His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
Plasmid#222991PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialPromotertacAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialPromoterT7Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT
Plasmid#26106PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis, TEV cleavage site, and TrxAExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2S
Plasmid#15807DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-200u
Plasmid#13555DepositorInsertFLIPPi G162A
TagsECFP and EYFPExpressionBacterialMutationG162A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAJM.715
Plasmid#108516PurposePTac-YFP Reporter. IPTG sensor for use in Marionette strain.DepositorInsertPTac-YFP
ExpressionBacterialAvailable SinceJune 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
luxB:cp157Venus+luxA/pRSETB Duet
Plasmid#197583PurposeBRET lux luciferase with cp157Venus for bacterial expressionDepositorInsertLuxA LuxBcp157Venus
UseE.coli expressionTagsHis X 6ExpressionBacterialPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21.RB
Plasmid#201007PurposepLv1-pUC18R6KT-miniTn7-Gm-mCherry-TagBFP, GmR, ApRDepositorInsertsmcherry
TagBFP
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840AAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R43W_E93G
Plasmid#109406PurposeRepA MutantDepositorInsertrepA
TagsRFPExpressionBacterialMutationR43W and E93G repA mutations to increase copy num…Available SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB27B
Plasmid#25545PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-Padron2
Plasmid#172355PurposeExpresses the reversibly switchable fluorescent protein Padron2 in bacterial cellsDepositorInsertPadron2
Tags6xHisTag (N terminal on backbone)ExpressionBacterialPromoteraraBADAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCT02
Plasmid#212152PurposePlasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.DepositorInsertFAST-PETase
Tags6x HisExpressionBacterialMutationS121E, D186H, R224Q, N233K, R280APromoterPT7Available SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AB-GFP
Plasmid#72203Purposescreen AB42 variants for aggregationDepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
ExpressionBacterialAvailable SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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M13KO7-ΔM13-ΔgeneIII-ΔgeneVI
Plasmid#80840PurposeModified helper phageDepositorInsertsM13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV
ExpressionBacterialAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET N6his GFP1-9
Plasmid#182240PurposeTripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.DepositorInsertGFP1-9
Tags6HISExpressionBacterialPromoterT7Available SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11.B
Plasmid#201005PurposepLv1-pUC18R6KT-miniTn7-Gm-TagBFP, GmR, ApRDepositorInsertTagBFP
ExpressionBacterialPromoterJ23104Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-emiRFP670-HO1
Plasmid#197252PurposeExpresses the protein of emiRFP670 and HO1 in bacteriaDepositorInsertemiRFP670
ExpressionBacterialAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AK1-His
Plasmid#124135PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-HyPerFLEX
Plasmid#217569PurposeBacterial expression of HyPerFLEXDepositorInsertHyPerFLEX
Tags6xHisExpressionBacterialPromoterT5Available SinceOct. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSSBIO32_dCas9_RPA1786
Plasmid#240489PurposeCRISPRi-mediated Knockdown of RPA1786DepositorInsertCas9 (cas9 Streptococcus pyogenes)
UseCRISPRExpressionBacterialMutationD10A, H840APromoterPlacAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW335p3
Plasmid#237329Purposeexpresses Z. mays RbcL RbcS-6xHisDepositorInsertsZ. mays RbcL
Z. mays RbcS-6xHis
Tags6xHis and NoneExpressionBacterialAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET24a_SMOX
Plasmid#186345PurposeC-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cellsDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29-1A(lacZ)
Plasmid#126978PurposeLevel 1 vector. With lacZ as alternative cloning reporter. OriV 9 (pBBR322/ROP; medium copy number).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM200
Plasmid#13079DepositorInsertPtac-gam-bet-exo operon
ExpressionBacterialAvailable SinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-EYFP
Plasmid#218952PurposeExpresses proinsulin fused to EYFP in bacteriaDepositorInsertproinsulin-EYFP
TagsproinsulinExpressionBacterialPromoterT5Available SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT FL USP7
Plasmid#227214PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialPromoterT7-promoterAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_nat
Plasmid#184911PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023
Plasmid#90324PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoterDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB46 (CK2beta)
Plasmid#27085DepositorAvailable SinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBad-mCherry-XL (R125K)
Plasmid#188054PurposeExpresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteriaDepositorInsertmCherry-XL (R125K)
Tags6xHisExpressionBacterialMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoteraraBADAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
ExpressionBacterialAvailable SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
ExpressionBacterialPromoterT7Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (ErmR)
Plasmid#177206PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
ExpressionBacterialPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-P2A-EGFP_mRFPstuf
Plasmid#137730PurposeExpresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNADepositorTypeEmpty backboneUseCRISPR, Lentiviral, Mouse Targeting, and Syntheti…ExpressionBacterial and MammalianAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringExpressionBacterialAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SrtAdelta59
Plasmid#51138Purposeexpression plasmid for N-terminal his tagged S.aureus SrtA delta 59DepositorInsertS.aureus SrtA delta 59
Tags6x HisExpressionBacterialPromoterT7Available SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_mic
Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9_ctrl
Plasmid#100981PurposeExpresses ThermoCas9 and its sgRNA moduleDepositorInsertsCas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialPromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LY-MT-P2A
Plasmid#214897PurposeExpresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sitesDepositorInsertSPLICSFAST-LY-MT-P2A
TagsnoneExpressionBacterial and MammalianAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-SP6
Plasmid#102418PurposeAc transposase with SP6 promoter for in vitro transcriptionDepositorInsertAc transposase
ExpressionBacterialPromoterSP6Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET23a-mUbc9
Plasmid#53137PurposeE.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)DepositorInsertUbc9 (Ube2i Mouse)
TagsuntaggedExpressionBacterialMutationprotein sequence is identical to humanPromoterT7Available SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCgCas9_recT
Plasmid#153337PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; KanrDepositorInsertCas9
UseCRISPRExpressionBacterialAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD-SepRS-EFSep
Plasmid#34623DepositorExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtac and Ptac (from SepRS- operon like)Available SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only