32,672 results
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Plasmid#228996PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-2
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaGIgG-12
Plasmid#228999PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaGIgG-12
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-3
Plasmid#228985PurposeFor bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-3
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB134
Plasmid#228485Purpose"Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)DepositorTypeEmpty backboneTags6xHis-TEV-Halo-HRVExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTBY1-T4uvsY
Plasmid#228214PurposeExpression in BL21(DE3) of T4 UvsY-like recombination mediatorDepositorInsertuvsY (uvsY Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-AffiHER2-SpyTag
Plasmid#216280PurposeExpresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expressionDepositorInsertSnoopTag-AffiHER2-SpyTag
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, SpyTag, and Thrombin cleavage sitā¦ExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD WT
Plasmid#164022PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARDDepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-ERK2
Plasmid#47573PurposeBacterial expression plasmid for GST-3xFlag-tagged ERK2DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 muā¦PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6-AP180
Plasmid#63006Purposeexpresses the adaptor protein AP180 in bacteriaDepositorAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXK-1/1
Plasmid#91539PurposeProtein expression and purification of human SH3 domain construct TXK-1/1DepositorInsertTXK-1/1 (TXK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TEC-1/1
Plasmid#91252PurposeProtein expression and purification of human SH3 domain construct TEC-1/1DepositorInsertTEC-1/1 (TEC Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ITK-1/1
Plasmid#91253PurposeProtein expression and purification of human SH3 domain construct ITK-1/1DepositorInsertITK-1/1 (ITK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rec-PhoA/CsgA
Plasmid#170787PurposeRecombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinaseDepositorUseSynthetic BiologyTagsHHHHHHExpressionBacterialPromoterInverted proD with Bxb1recognition sites and Inveā¦Available SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_2
Plasmid#78206PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter bindingDepositorInsertmatB (His6 C106A S170A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A S170A R286C Q457C K488APromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
TAF1L
Plasmid#39014PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-trAtCCD7-OsD27(pYL726 )
Plasmid#178285PurposeCo-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from ArabidopsisDepositorInsertsOsD27
trAtCCD7
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-trAtCCD8(pYL735)
Plasmid#178286PurposeExpresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJS005
Plasmid#188328PurposePCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mNeonGreen::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
B7-5
Plasmid#111493Purposetranslational initiation factor 1DepositorInserttranslational initiation factor 1
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only