30,880 results

1. pQE80_T5_Z18-ehaA_LacI

   Plasmid

   #186314

   Purpose

   Plasmid for inducible E coli expression of the Z18 helixCAM.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Insert

   Z18-ehaA
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCoofy5

   Plasmid

   #43989

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His6-Sumo1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-MinC

   Plasmid

   #133628

   Purpose

   Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

   Insert

   His-MinC (minC E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6ASB (FBXL19)

   Plasmid

   #119954

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   FBXL19 (FBXL19 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 31-153

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCPP5588

   Plasmid

   #128708

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopD1
   

   Expression

   Bacterial

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCPP5053

   Plasmid

   #128707

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopC1
   

   Expression

   Bacterial

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPP5530

   Plasmid

   #128705

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopA1
   

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-GFP-CPDSalI

   Plasmid

   #38257

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   eGFP
   

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pEGFPN3 (Clonetech)

   Promoter

   T7

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pETDuet RagA(T21N)-RagC

   Plasmid

   #99644

   Purpose

   Bacterial expression of RagA(T21N)-RagC

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   T21N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. APneg-SNAP25d

    Plasmid

    #168014

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pE4c

    Plasmid

    #17464

    Purpose

    modified pENTR vector for C-terminal GST tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    GST

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. PUM1_PUM_pGEX

    Plasmid

    #135129

    Purpose

    Recombinant expression of PUM1 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    PUM1 (PUM1 Human)
    

    Tags

    GST-SBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQLinkHD_mVenus

    Plasmid

    #118861

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    mVenus
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKS104

    Plasmid

    #24632

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    fadD (M3351), atfA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-MRPP1

    Plasmid

    #67862

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP1 (TRMT10C Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmRFPmars

    Plasmid

    #26252

    Depositor

    Martin Fraunholz

    Article

    Paprotka et al J Microbiol Methods. 2010 Oct;83(1):82-6

    Insert

    mRFPmars
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    KpnI restriction site removed

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

17. C1QBP

    Plasmid

    #32863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    C1QBP (C1QBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids E89-Q282 only

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pNH-TrxT-G-S18

    Plasmid

    #127827

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-p40R57Q PX

    Plasmid

    #19009

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p40phox (NCF4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa1-148 (PX domains) site directed mutagenesis: …

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pRSF-G1(6CNW)RS

    Plasmid

    #222873

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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