30,245 results

1. pGEX6P1-HA-MEK5

   Plasmid

   #53166

   Purpose

   Expresses GST-HA tagged human MEK5 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK5 (MAP2K5 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPTF

   Plasmid

   #39111

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BPTF (BPTF Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. Fyn-SH3_5WT_S25C

   Plasmid

   #118824

   Purpose

   Bacterial expression of the SH3 domain of human Fyn

   Depositor

   Nicolas Fawzi

   Article

   Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

   Insert

   FYN (FYN Human)
   

   Use

   Tags

   6-His-TEV tag

   Expression

   Bacterial

   Mutation

   S25C; See depositor comments below.

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. RTA

   Plasmid

   #64029

   Purpose

   Expression of Ricin A Chain (RTA) in E. coli

   Depositor

   Vern Schramm

   Article

   Ho et al Proc Natl Acad Sci U S A. 2009 Dec 1;106

   Insert

   Ricin A Chain
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pEmpty

   Plasmid

   #214051

   Purpose

   Empty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas system

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. RP1B FUS 1-163 S12xQ

   Plasmid

   #127193

   Purpose

   Protein expression for affinity purification

   Depositor

   Nicolas Fawzi

   Article

   Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

   Insert

   FUS 1-163 S12xQ (FUS Human)
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   S12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx-mCherry-Zdk1-Rac1 Q61L

   Plasmid

   #81059

   Purpose

   mammalian expression of Zdk1 tagged Rac1 Q61L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   Rac1 Q61L (RHOA Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pWJB30

   Plasmid

   #126505

   Purpose

   Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

   Depositor

   Ute Romling

   Article

   Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

   Insert

   adrA-8xHis
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD30

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. A0A075HNX4

   Plasmid

   #177695

   Purpose

   expresses a fatty alcohol oxidase in E. coli

   Depositor

   Martin Engqvist

   Article

   Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

   Insert

   Long chain alcohol oxidase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBXC3GH

    Plasmid

    #47070

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSiMPla_N + pSiMPla_C

    Plasmid

    #134314

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    ampR_gp41-1 N-intein + ampR_gp41-1 C-intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL1-MBP-4xDQNAT

    Plasmid

    #128390

    Purpose

    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Use

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    A302T- please see depositor comment

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSETT4gap

    Plasmid

    #153413

    Purpose

    φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

    Depositor

    Joern Kalinowski

    Article

    Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gapDH promoter from Eggerthella lenta

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRE-Tn5-162

    Plasmid

    #118543

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-AR-1-660

    Plasmid

    #104196

    Purpose

    Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-660 (AR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. t1-405

    Plasmid

    #166127

    Purpose

    For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    Tln1Head (1-405) (TLN1 Human)
    

    Use

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-405 only

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dhh1

    Plasmid

    #201412

    Purpose

    Bacterial protein expression of Dhh1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    MBP, TEV and TEV, 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4046809

    Plasmid

    #145685

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. C163

    Plasmid

    #141127

    Purpose

    Expresses sfCFP under lac promoter

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Insert

    sfCFP
    

    Use

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    engineered lac promoter

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. C164

    Plasmid

    #141128

    Purpose

    Expresses sfYFP under lac promoter

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Insert

    sfYFP
    

    Use

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    engineered lac promoter

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge