33,483 results
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Plasmid#53208PurposeExpressed N-terminally Hexahistine tagged TRF2 Delta Basic proteinDepositorInsertTRF2 Delta Basic (TERF2 Human)
Tags6x HisExpressionBacterialMutationcontains amino acids 47-500PromoterTrcAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BLMA-c800
Plasmid#53631Purposebacterial expression plasmidDepositorInsertBLMA
TagsHis-TevExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 High pI protein 85 TEV LIC cloning vector (2-HpI-85)
Plasmid#55213DepositorTypeEmpty backboneTags6xHis, High pI protein 85, and TEV cleavage siteExpressionBacterialPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL030
Plasmid#53533Purposesupplies EtpC required for optimal EtpA expression on compatible plasmidDepositorInsertetpC
UsePacyc184ExpressionBacterialAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(cAMP-3.3)/pRSETB
Plasmid#53592PurposeNano-lantern-based luminescent cAMP indicatorDepositorInsertNano-lantern-based luminescent cAMP indicator
Tags6xHisExpressionBacterialAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
ETV1A-c014
Plasmid#53655Purposebacterial expression plasmidDepositorInsertETV1A
ExpressionBacterialMutationBacterial expression for structure determination;…Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
ASB9A-c009
Plasmid#53670Purposebacterial expression plasmidDepositorInsertASB9A
TagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B DN
Plasmid#49529Purposecloning vector for dominant negative chick BMP receptor 1BDepositorPublicationInsertBMPR-1B DN (BMPR1B Chicken)
ExpressionBacterialMutationdominant negative mutation (K231R); also P2A, M11…PromoterT3Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 651
Plasmid#53016PurposeLacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).DepositorInsertsLacIq
tetR
UseSynthetic BiologyTagsRBS(st3) and T1 terminatorExpressionBacterialPromoterpConAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1454
Plasmid#53019PurposeAmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertfimB
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlac0-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1277
Plasmid#53017PurposeAmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1DepositorInsertsGFP
mCherry
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR4-Dact1(1250-1601)
Plasmid#52046PurposeTo generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.DepositorPublicationInsertDact1(1250-1601) (Dact1 Mouse)
ExpressionBacterialAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-hMeCP2-R270X
Plasmid#48092PurposeBacterial expression of Mxe intein/chitin binding domain tagged MeCP2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
ExpressionBacterialMutationMxe intein/chitin binding domain tagged, expresse…Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
psbA2(noATbox)-PHLS (c)
Plasmid#52308PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyExpressionBacterialMutationCAAATACA box in the psbA2 promoter to ggcgcgccPromoterpsbA2Available sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClbN-NHis
Plasmid#51497PurposeNRPS module containing CATE domains with N-terminal 6His tagDepositorInsertClbN
Tags6x His tag and T7 tagExpressionBacterialMutationcontains aa30-1094 of NP_754347.1PromoterT7Available sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CT-Nano-lantern(ATP1)/pRSETB
Plasmid#51990Purposebacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplastsDepositorInsertNano-lantern_ATP1
Tags6xHis and chloroplast transit peptidExpressionBacterialMutationS257G in Rluc8Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(ATP1)/pRSETB
Plasmid#51988Purposebacterial expression of a Nano-lantern-based luminescent ATP indicatorDepositorInsertNano-lantern_ATP1
Tags6xHisExpressionBacterialMutationS257G in Rluc8Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbP
Plasmid#48244PurposeUntagged, full-length protein for in vivo peptidase assaysDepositorInsertClbP
TagsNoneExpressionBacterialPromoterT7Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET29b-ClbPpep-CHis
Plasmid#48243PurposeFirst 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domainDepositorInsertClbP-pep
Tags6-HisExpressionBacterialMutationFirst 375 amino acids of the peptidase, which con…PromoterT7Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only