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pJExpress401-KDAC8

Plasmid

#224208

Purpose

Expresses human KDAC8 (HDAC8) in E. coli

Depositor

Terry Watt

Publication

Toro et al Protein Sci. 2015 Dec;24(12):2020-32. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Promoter

T5

Available since

Dec. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGD289

Plasmid

#228113

Purpose

pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

Depositor

Seth Shipman

Publication

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Kva1 retron, ncRNA with 90 bp rpoB donor

Expression

Bacterial

Promoter

T7

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJL NarL YdfI Flag

Plasmid

#225650

Purpose

Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.

Depositor

Neha Kamat

Publication

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

NarL-YdfI (narL Synthetic)

Use

Cell free expression

Tags

FLAG

Expression

Bacterial

Mutation

NarL with native DNA-binding domain replaced with…

Promoter

T7

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTW358f

Plasmid

#227935

Purpose

Express hIAPP-T7n

Depositor

Tina Wang

Publication

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

hIAPP-T7n

Expression

Bacterial

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTW357b

Plasmid

#227930

Purpose

Low-stringency accessory plasmid for selection

Depositor

Tina Wang

Publication

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Expression

Bacterial

Mutation

R632S

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTW357bN

Plasmid

#227932

Purpose

Accessory plasmid for negative selection

Depositor

Tina Wang

Publication

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII-neg
T7c

Expression

Bacterial

Mutation

R632S

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTW357d5

Plasmid

#227931

Purpose

High-stringency accessory plasmid for selection

Depositor

Tina Wang

Publication

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Expression

Bacterial

Mutation

R632S, Q649S

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHelper-CBE-sgRNA

Plasmid

#221137

Purpose

Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

Depositor

Craig Roy

Publication

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

sgRNA-scaffold with Cas9 handle

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI)

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-roGFP

Plasmid

#202464

Purpose

Expresses redox sensitive GFP (roGFP) with T7 induction

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Tags

6X-His

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM236 Ubc4

Plasmid

#226361

Purpose

Expression of yeast Ubc4

Depositor

Andreas Martin

Publication

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

Ubc4 (UBC4 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pR_Lux

Plasmid

#226457

Purpose

lambda immunity region to the luciferase operon (lux)

Depositor

Emily Balskus

Publication

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

lambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens

Expression

Bacterial

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-LCC

Plasmid

#218686

Purpose

Encodes codon optimized LCC enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

LCC

Tags

10xHis

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-acatenin

Plasmid

#186458

Purpose

Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Publication

Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

Insert

CTNNA1 (CTNNA1 Human)

Tags

GST

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-AtUBC8-S-AtUBA1-S

Plasmid

#222714

Purpose

Expression AtUBA1-S and AtUBC8-S in in bacterial cells

Depositor

Lei Wu

Publication

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtUBC8 (UBC8 Mustard Weed)
ATUBA1 (UBA1 Mustard Weed)

Tags

S-Tag

Expression

Bacterial

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-HIS-FLAG-AtUBQ11

Plasmid

#222720

Purpose

Expression HIS-FLAG-AtUBQ11 in in bacterial cells

Depositor

Lei Wu

Publication

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtUBQ11 (UBQ11 Mustard Weed)

Tags

2xFLAG and 6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-FBXL5-SKP1

Plasmid

#226620

Purpose

Co-expression of FBXL5-SKP1 complex in E.coli

Depositor

Ning Zheng

Publication

Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

Inserts

FBXL5 (FBXL5 Human)
SKP1 (SKP1 Human)

Tags

GB1

Expression

Bacterial

Mutation

Amino acids 1-198 was deleted, and 420-596 was re…

Promoter

T7

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNHA7

Plasmid

#223649

Purpose

birA protein purification

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BioID_G118R

Tags

6His

Expression

Bacterial

Mutation

G118R

Promoter

LacI

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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WDV-Echistatin

Plasmid

#226985

Purpose

Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Depositor

Wendy Gordon

Publication

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Inserts

WDV (repA )
Echistatin

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAP003l2

Plasmid

#209748

Purpose

RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

Depositor

Ahmed Badran

Publication

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-C-mScarlet-Zeo-TSC22D2

Plasmid

#222670

Purpose

endogenously tag TSC22D2 with C-terminal mScarlet

Depositor

Jason Moffat

Publication

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Tags

mScarlet

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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