30,735 results

1. GB1-PRD(703-800)-Strep

   Plasmid

   #141344

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1-His6-TEV-ALIX(703-800)-Strep
   

   Tags

   GB1-His6-TEV and Strep-tag (WSHPQFEK)

   Expression

   Bacterial

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV11K

   Plasmid

   #63184

   Purpose

   pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SMYD3

   Plasmid

   #25318

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SMYD3_26 (SMYD3 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pTriEx-mCherry-LINXb3

   Plasmid

   #73615

   Purpose

   Light-induced nuclear export switch for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXb3
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRD119 (PCNA GFP)

   Plasmid

   #14822

   Depositor

   Robert Duronio

   Article

   Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

   Insert

   PCNA-GFP (PCNA Fly)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   July 30, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pBbA5k-RFP

   Plasmid

   #35282

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB2-Katushka-9-5

   Plasmid

   #104011

   Purpose

   Plasmid encoding Katushka-9-5

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Katushka9-5
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (K6S, N21D, R32G, L…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXZX352

   Plasmid

   #169456

   Purpose

   MATA fragment in pTOPO vector

   Depositor

   Jef Boeke

   Article

   Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

   Insert

   Mata locus
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXZX353

   Plasmid

   #169457

   Purpose

   MATα (alpha) fragment in pTOPO vector

   Depositor

   Jef Boeke

   Article

   Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

   Insert

   MatA
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

    Plasmid

    #190898

    Purpose

    Entry vector for multiple sgRNA cloning

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6 and 7sk

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJW4.04

    Plasmid

    #171209

    Purpose

    bacterial expression of MBP-tagged PGL-3

    Depositor

    Geraldine Seydoux

    Article

    Schmidt et al bioRxiv 2020.10.15.340570

    Insert

    pgl-3 (pgl-3 Nematode)
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-OleD

    Plasmid

    #59372

    Purpose

    Inducible expression of OleD (DQ195536) under arabinose promoter

    Depositor

    Christopher Anderson

    Insert

    OleD-His
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH-macroHC

    Plasmid

    #82337

    Purpose

    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli

    Depositor

    Michael Hothorn

    Article

    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p

    Insert

    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    

    Tags

    6xHis non-cleavable and macro domain of human his…

    Expression

    Bacterial

    Mutation

    Truncated protein, amino acids 1-178

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T2 SHP2 E76A

    Plasmid

    #8324

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E76A (activated mutant)

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH291

    Plasmid

    #172153

    Purpose

    Reporter plasmid for pylBCD variant in pCDF backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para sfGFP.2TAG; Plpp pylS; PproK pylT; AraC
    

    Expression

    Bacterial

    Mutation

    sfGFP(N39*, Y151*)

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. human rSHIP2 (18-893)

    Plasmid

    #128568

    Purpose

    Expression of human SHIP2 in bacteria

    Depositor

    Christophe Erneux

    Article

    Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

    Insert

    SHIP2 (INPPL1 Human)
    

    Tags

    His Xpress (N-terminal)

    Expression

    Bacterial

    Mutation

    SHIP2 coding sequence from amino acid 18 (A) till…

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. B2-3

    Plasmid

    #111470

    Purpose

    Lys-tRNA synthetase

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    Lys-tRNA synthetase
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD_mCherry_GFP_++

    Plasmid

    #187389

    Purpose

    arabinose-inducible expression of GFP and mCherry

    Depositor

    Roi Avraham

    Insert

    GFP, mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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