31,059 results
-
Plasmid#106095Purposebacterial protein expression of TIA1DepositorAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pGEX6P1-N-His
Plasmid#119755PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.DepositorTypeEmpty backboneTags10xHis and GSTExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-TEV-C9R
Plasmid#118754PurposeTEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activityDepositorInsertTobacco Etch Virus protease (NEWENTRY Synthetic)
TagsC9R and His tagExpressionBacterialPromoterT7Available SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/601
Plasmid#26656DepositorInsert
ExpressionBacterialAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneExpressionBacterialPromoterhyper-pSpankAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJA1
Plasmid#177655PurposeRecombinant protein synthesis of human DNAJA1DepositorAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin WT
Plasmid#12647DepositorAvailable SinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDIV151
Plasmid#226180PurposeExpresses Cas9 in a backbone compatible with K. phaffii.DepositorInsertCas9
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterGAP1Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM-LIN28
Plasmid#16350DepositorInsertHomo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
ExpressionBacterialAvailable SinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEGF
Plasmid#199231PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpidermal growth factor, partial (EGF Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
6BHD (SETDB1)
Plasmid#119955PurposeBacterial expression for structure determinationDepositorInsertSETDB1 (SETDB1 Human)
Tags6xHis-TEVExpressionBacterialMutationcontains amino acids 190-410PromoterT7Available SinceFeb. 1, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a-MetRS-His
Plasmid#124115PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mTagBFP2-TEV-Strep-His
Plasmid#210824PurposeBacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent proteinDepositorInsertmTagBFP2
TagsTwinStrep, 10xHisExpressionBacterialPromoterT7Available SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MBP-His-SFP
Plasmid#141141PurposeExpression of 4'-phosphopantetheinyl transferase geneDepositorInsert4'-phosphopantetheinyl transferase
TagsMBPExpressionBacterialAvailable SinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJYS2_crtYf
Plasmid#85544PurposeConstitutive transcription of crRNA of crtYf, Spectinomycin resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…ExpressionBacterialAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV- PRDX2
Plasmid#31338DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only