32,771 results
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Plasmid#239451Purposeexpress eforRed chromoproteinDepositorInserteforRed
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ3534 fwYellow
Plasmid#239452Purposeexpress fwYellow chromoproteinDepositorInsertfwYellow
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB413a
Plasmid#20058DepositorInsertSperm Whale Myoglobin
ExpressionBacterialAvailable SinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos36)GFP
Plasmid#62937PurposeExpression of 6xHis-(+36)GFP in bacterial cellsDepositorInsert(pos36)GFP
Tags6xHis tagExpressionBacterialPromoterT7Available SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-544
Plasmid#11519DepositorTypeEmpty backboneUseGateway CloningTagsHis6-NusAExpressionBacterialAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_repA1
Plasmid#211910PurposeReplicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM253 (miniCTX1-rhaSR-PrhaBAD)
Plasmid#110571PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
ExpressionBacterialPromoterrhaSR-PrhaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
ExpressionBacterialAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDae069 (eMutaT7[PmCDA1] with optimized RBS)
Plasmid#187621PurposeExpresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertPmCDA1-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTP955
Plasmid#104164PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT_D355N
Plasmid#46988PurposeInducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
TagsGST and PreScission cleavage siteExpressionBacterialMutationMutation aspartic acid 355 to asparagine (D355N);…PromotertacAvailable SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
ExpressionBacterialPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD3_mCherry
Plasmid#187385PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRTDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1
Plasmid#20668PurposeS. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.DepositorTypeEmpty backboneTagsGFP mut3ExpressionBacterialAvailable SinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMRE145
Plasmid#118497Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKL2299
Plasmid#186332PurposeThis plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.DepositorInsertvirulence genes from Agrobacterium tumefaciens pTiBo542
ExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pComb3XTT
Plasmid#63891PurposeBackbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)DepositorInsertTT Fab
UsePhage displayTags6xHis, HA tag, and geneIIIExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp-eyfp
Plasmid#65035PurposeTpR mini-Tn7 vector, mobilizable, for YFP tagging bacteriaDepositorInserteyfp
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis T4 DNA Ligase
Plasmid#206207PurposeInducible expression of T4 DNA ligaseDepositorInsertT4 DNA ligase
ExpressionBacterialAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD42-BtuCEDFB
Plasmid#237541Purposeplasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteinsDepositorInsertsbtuB
btuF
btuCED
ExpressionBacterialPromoterpBADAvailable SinceSept. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKL2315
Plasmid#187876PurposeExpresses Cre recombinase.DepositorInsertCre recombinase
UseCre/LoxExpressionBacterialPromoterJ23107Available SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.1-c002
Plasmid#173087PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
TagsmCeruleanExpressionBacterialMutationQ162-N369, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
NylCp2-TS_pET28a
Plasmid#215421PurposeExpression construct for NylCp2-TS gene, codon optimized for expression in E. coliDepositorInsertNylCp2-TS
ExpressionBacterialMutationD122G/H130Y/D36A/E263QAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-UFBP1
Plasmid#204558PurposeExpresses GST-tagged UFBP1 in E.coliDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1_mBlueberry2
Plasmid#248930PurposeBacterial expression of fluorescent membrane scaffold protein MSP1E3D1 fused to mBlueberry 2 for nanodisc formationDepositorInsertMSP1E3D1_mBlueberry2
TagsHisExpressionBacterialPromoterT7 PromoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKB225
Plasmid#170015PurposeEncodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Peptide display protein with no C-terminal peptide
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AraC/pBad-Tnpase (camR)
Plasmid#213909PurposeArabinose-inducible himar1C9 transposase expression - cam resistanceDepositorInsertAraC/pBad ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMyBADNT
Plasmid#84690PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAraCAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Traptavidin
Plasmid#24753DepositorInsertTraptavidin
TagsHis-6ExpressionBacterialMutationStreptavidin with 2 amino acid mutations: S52G an…Available SinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only