34,152 results

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pAKlux1

Plasmid

#14073

Depositor

Attila Karsi

Publication

Karsi et al FEMS Microbiol Lett. 2006 Jul. 260(2):21

Insert

Bacterial luciferase

Expression

Bacterial

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SIRT7.4

Plasmid

#13740

Depositor

John Denu

Publication

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT7 (SIRT7 Human)

Tags

His

Expression

Bacterial

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX4T1-Smurf2 wt

Plasmid

#13504

Depositor

Jeff Wrana

Publication

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

GST

Expression

Bacterial

Available since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pET26b-Granzyme A

Plasmid

#8823

Depositor

Judy Lieberman

Publication

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A (GZMA Human)

Tags

(His)6 and Enterokinase site

Expression

Bacterial

Mutation

it mature enzyme.

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-2T SHP1 WT

Plasmid

#8594

Depositor

Ben Neel

Publication

Plutzky et al Proc Natl Acad Sci U S A 1992 Feb 1;89(3

Insert

SHP1 (PTPN6 Human)

Tags

GST

Expression

Bacterial

Available since

June 21, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET21b FAST-PETase

Plasmid

#225760

Purpose

Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Publication

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

FAST-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEcCas-2.0

Plasmid

#190697

Purpose

Constitutive expressing of Cas9 and inducible expression of lambda Red

Depositor

Sheng Yang

Publication

Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

Insert

Cas9 and Lamda Red system

Expression

Bacterial

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-MCN-10His-mStayGold (E138D)

Plasmid

#211361

Purpose

Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Publication

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Bacterial

Mutation

E138D

Promoter

T7

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Publication

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28c S2

Plasmid

#171920

Purpose

Express RNA-based (p)ppGpp sensor

Depositor

Mingxu You

Publication

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Insert

RNA-based (p)ppGpp sensor

Expression

Bacterial

Promoter

T7

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBad-LgBiT-PhoCl1-SmBiT-MBP

Plasmid

#164034

Purpose

luciferase-based PhoCl screening construct

Depositor

Robert Campbell

Publication

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

LgBiT-PhoCl1-SmBiT-MBP

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pPP537

Plasmid

#158803

Purpose

pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Publication

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-6xHis

Expression

Bacterial

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Publication

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Expression

Bacterial

Mutation

E36K

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNCST-AausFP1

Plasmid

#129499

Purpose

Expresses AausFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRSET-QUE2m

Plasmid

#129350

Purpose

ATP measurement

Depositor

Hiroyuki Noji

Publication

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-2m

Expression

Bacterial

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSCM001

Plasmid

#124605

Purpose

Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

Depositor

David Summers

Publication

Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

Insert

mCherry-pHluorin

Tags

mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

Expression

Bacterial

Promoter

pDAB

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMRE-Tn7-152

Plasmid

#118566

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNO286-3

Plasmid

#107746

Purpose

Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

Depositor

Jeffrey Tabor

Publication

Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Mutation

Deleted AA 223 and 512

Promoter

J23106 and J23108

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-MH6-RspCas13d_RspCasWYL1

Plasmid

#108304

Purpose

Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Publication

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Inserts

RspCas13d
RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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