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pET28a-MH6-RspCas13d_RspCasWYL1

Plasmid

#108304

Purpose

Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Publication

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Inserts

RspCas13d
RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a_T7-ARG2

Plasmid

#106474

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

Depositor

Mikhail Shapiro

Publication

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET251-pUC 12xMS2V6 Loxp KANr Loxp

Plasmid

#104392

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Publication

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEB2-E2-Crimson

Plasmid

#104010

Purpose

Plasmid encoding E2-Crimson

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

E2-Crimson

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher002

Plasmid

#102829

Purpose

Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

Depositor

Mark Howarth

Publication

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Cys-SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Cysteine residue in N-terminal region

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET15bHis6Tnp

Plasmid

#79807

Purpose

Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

Depositor

Keji Zhao

Publication

Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

Insert

His6Tnp

Tags

His

Expression

Bacterial

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Publication

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Expression

Bacterial

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-IG-mSA

Plasmid

#80706

Purpose

Expresses monomeric streptavidin (mSA) in bacterial cells

Depositor

Matthieu Sainlos

Publication

Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

Insert

Monomeric streptavidin

Tags

10xHis tag and TEV site

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pET-(-30)GFP-9xGGS-Cre-6xHis

Plasmid

#62372

Purpose

Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP-9xGGS-Cre-6xHis

Tags

(-30)GFP and 6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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Venus-pBAD

Plasmid

#54859

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

6xHis and Venus

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
Sirius/pRSETB

Plasmid

#51956

Purpose

bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Publication

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Tags

6xHis

Expression

Bacterial

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHT-ads

Plasmid

#47382

Purpose

Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

Depositor

Heng-Phon Too

Publication

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Insert

ads

Tags

6xHis

Expression

Bacterial

Promoter

Pgrac

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-3xMBT_D355N

Plasmid

#46988

Purpose

Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli

Depositor

Or Gozani

Publication

Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

Insert

Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)

Tags

GST and PreScission cleavage site

Expression

Bacterial

Mutation

Mutation aspartic acid 355 to asparagine (D355N);…

Promoter

tac

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Publication

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBAD-PSmOrange

Plasmid

#31886

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Expression

Bacterial

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His6 MBP Asn10 TEV LIC cloning vector (1C)

Plasmid

#29654

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xAsn, 6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Publication

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Bacterial, Mammalian, and Y…

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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UEV1

Plasmid

#25619

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

UEV1 (UBE2V1 Human)

Tags

His

Expression

Bacterial

Available since

Aug. 31, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles