34,152 results
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Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyExpressionBacterialPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
Tags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389Available sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB1-mVenus NB
Plasmid#103986PurposePlasmid encoding mVenus NBDepositorInsertmVenus NB
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
TagsHisExpressionBacterialAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-CTG-47
Plasmid#99150Purpose47xCAG repeats in pBluescript vectorDepositorInsert47xCTG
ExpressionBacterialPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
ExpressionBacterialAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pG1AK-sfGFP
Plasmid#71739PurposeModular shuttle vector for Geobacillus and E. coliDepositorPublicationInsertsfGFP
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGSKU
Plasmid#72243PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
kanMX4
I-SceI
ExpressionBacterialAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)GFP-9xGGS-Cre-6xHis
Plasmid#62372PurposeExpression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cellsDepositorInsert(-30)GFP-9xGGS-Cre-6xHis
Tags6xHis tag and (-30)GFPExpressionBacterialPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Venus-pBAD
Plasmid#54859PurposeLocalization: Protein Expression Vector , Excitation: 515, Emission: 528DepositorTypeEmpty backboneTags6xHis and VenusExpressionBacterialAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Sirius/pRSETB
Plasmid#51956Purposebacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent proteinDepositorInsertSirius
Tags6xHisExpressionBacterialAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21-Streptavidin-Glutamate_Tag
Plasmid#46367DepositorInsertStreptavidin-Glutamate_Tag
Tags6 glutamate tagExpressionBacterialPromoterT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-3xMBT_D355N
Plasmid#46988PurposeInducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
TagsGST and PreScission cleavage siteExpressionBacterialMutationMutation aspartic acid 355 to asparagine (D355N);…PromotertacAvailable sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINE-3C-eGFP
Plasmid#41125DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
ExpressionBacterial, Insect, and Mamm…Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BRD4
Plasmid#38943PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSP1E3D1_D73C
Plasmid#39328DepositorInsertMSP1E3D1_D73C (APOA1 synthetic gene)
Tags6xHisExpressionBacterialMutationcys mutant of MSP1E3D1PromoterT7Available sinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-PSmOrange
Plasmid#31886DepositorInsertPSmOrange
ExpressionBacterialMutationS21T, Q63L, F100Y, L125M, K166R, P192S (mutations…Available sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET His6 MBP Asn10 TEV LIC cloning vector (1C)
Plasmid#29654DepositorTypeEmpty backboneTags6xHis, 10xAsn, MBP, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by