30,695 results

1. pET28a_TIA1

   Plasmid

   #106095

   Purpose

   bacterial protein expression of TIA1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   TIA1 (TIA1 Human)
   

   Tags

   6xHIs-thrombin and T7

   Expression

   Bacterial

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-ATG8(416)/GFP-AUT7(416)

   Plasmid

   #49425

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

   Depositor

   Daniel Klionsky

   Article

   Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Promoter

   ATG8

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE80l ACA

   Plasmid

   #196471

   Purpose

   Protein expression of SpyTag-ELP-CarHC-ELP-SpyTag

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   ACA
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRBN_ΔHBD

   Plasmid

   #232779

   Purpose

   Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

   Depositor

   Ivan Dikic

   Article

   Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

   Insert

   Cereblon (CRBN Human)
   

   Tags

   MBP-His6-TEV

   Expression

   Bacterial

   Mutation

   CRBN (residues 47 to 193 and 249 to 436) with GNG…

   Promoter

   T7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBECKP-km

   Plasmid

   #117235

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEP PolI

   Plasmid

   #11722

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I EP (polA E. coli)
   

   Expression

   Bacterial

   Mutation

   D424A, I709N, A759R. Error-prone version of PolI.

   Available Since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUltra-5HTP

   Plasmid

   #178042

   Purpose

   Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

   Depositor

   Han Xiao

   Article

   Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

   Inserts

   Modified ScTrpRS for 5HTP incoporation
   suppressor tRNA for 5HTP incorporation
   

   Expression

   Bacterial

   Mutation

   T107C, P254T, C255A

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST14-Cys-SpyCatcher002

   Plasmid

   #102829

   Purpose

   Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Cys-SpyCatcher002
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Cysteine residue in N-terminal region

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Plasmid

   #159540

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   G12C

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTXTL-T7max-RLuc

    Plasmid

    #188312

    Purpose

    Expression of Renilla luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. HIS-HA-CMYC P53

    Plasmid

    #233738

    Purpose

    expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

    Depositor

    Albert Keung

    Article

    Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

    Insert

    HIS-HA-CMYC-p53 (TP53 Human)
    

    Tags

    HIS-HA-CMYC

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Rat, Human, Mouse, Bovine)
    

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Promoter

    T7 polymerase

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCURE

    Plasmid

    #23013

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    I-CreI homing endonuclease
    

    Expression

    Bacterial

    Mutation

    I-CreI expression is controlled by the arabinose …

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbS8k-RFP_N99D

    Plasmid

    #109410

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    N99D repA mutation to increase copy number

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS mTSP-2

    Plasmid

    #12999

    Depositor

    Jack Lawler

    Insert

    TSP-2 (Thbs2 Mouse)
    

    Expression

    Bacterial

    Available Since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pTW411d

    Plasmid

    #246585

    Purpose

    different combination of A. thaliana and N. tabacum Rubisco assembly factors

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    NtRaf1, NtRaf2, NtRbcX2, NtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30a-GABAT

    Plasmid

    #239572

    Purpose

    Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

    Depositor

    William Plaxton

    Article

    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

    Insert

    4-aminobutyrate aminotransferase (gabT E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYFJ84

    Plasmid

    #227644

    Purpose

    Vibrio harveyi acyl-ACP synthetase

    Depositor

    John Cronan

    Article

    Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

    Insert

    Acyl-Acyl carrier protein synthetase
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDF-Leup

    Plasmid

    #213154

    Purpose

    Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

    Depositor

    Jason Crawford

    Article

    Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

    Insert

    Leupeptin Protease Inhibitor Pathway
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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