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pBS mFgfr1 (CT#92)

Plasmid

#14005

Depositor

Cliff Tabin

Insert

Fgfr1 (Fgfr1 Mouse)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pGEX5X.1 PINK1 KD

Plasmid

#13322

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496; Kinase dead: K219D/D362A/D3…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pBMM42

Plasmid

#13331

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

XRCC4 (XRCC4 Human)

Tags

His

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Smad3

Plasmid

#12630

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
p3035 GST-HPV-18 E6

Plasmid

#10850

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

HPV18 E6 (E6 Human papillomavirus)

Tags

GST

Expression

Bacterial

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pET21b FAST-PETase

Plasmid

#225760

Purpose

Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

FAST-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHEN2-SG4 R105A

Plasmid

#196072

Purpose

Express mutant (R105A) SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4 R105A

Tags

3XFLAG and 6XHis

Expression

Bacterial

Mutation

R105A mutation in SG4

Promoter

T7

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-RT003-GPM

Plasmid

#163383

Purpose

To express sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

GFP-PDGFRtm-2A-mCherry (GPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT

Plasmid

#154271

Purpose

Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

Insert

O-GlcNAc Transferase (OGT Human)

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

bacterial codon optimized

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJeM1

Plasmid

#135088

Purpose

rhamnose-inducible expression vector for replication in E. coli and P. putida

Depositor

Josef Altenbuchner

Article

Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

Insert

rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pGNW2

Plasmid

#122086

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLBH559_Tet-HisCas14a1Locus

Plasmid

#112502

Purpose

Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

6xHis-TEV

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2

Plasmid

#113634

Purpose

An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

Depositor

Miguel Valvano

Article

Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

Type

Empty backbone

Expression

Bacterial

Promoter

PrhaB

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCAP05

Plasmid

#102920

Purpose

Direct cloning and broad-host-range heterologous expression vector

Depositor

Bradley Moore

Article

Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pST39-pNL29

Plasmid

#91696

Purpose

Expresses Mega GVs in E.Coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

Insert

Mega GV gene cluster (from pNL29) in pST39 backbone

Tags

No tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-NT

Plasmid

#89960

Purpose

Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

empty gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-Spy

Plasmid

#90102

Purpose

IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YcgR (ycgR )

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pTlpA39-Wasabi

Plasmid

#86116

Purpose

mWasabi reporter gene gated by TlpA39

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA39
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, D135V, A217V, L236F

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Tags

OneSTrEP-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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