30,535 results

1. pET11a_3xFlag-nsp14 (SARS-CoV-2)

   Plasmid

   #169159

   Purpose

   To express SARS-CoV-2 nsp14 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX4T3-GST-Tev

   Plasmid

   #177142

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   Tev
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOSIP-KC (KanR, PhiC31)

   Plasmid

   #45982

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Ursula Jakob, Michael Gray

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   6X His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK302

   Plasmid

   #87768

   Purpose

   As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

   Inserts

   PrhaBAD-sfGFP
   rhaS from E. coli with its native RBS from E. coli
   

   Use

   Synthetic Biology

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD rhamnose-inducible promoter from E. coli …

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYC-TtCas6-4xcrRNA4.5

   Plasmid

   #127764

   Purpose

   For expression of T. thermophilus Csm complex in E. coli (crRNA)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Synthetic CRISPR array containing four identical T. thermophilus spacers
   Cas6A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. UbcH5c S22R pET28a

   Plasmid

   #12644

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   Ubch5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

8. Opto-CRAC version 1

   Plasmid

   #101245

   Purpose

   Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

   Depositor

   Yubin Zhou

   Article

   He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

   Insert

   LOV2 and STIM1 cytosolic fragment
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDFDuet-MKK6-EE p38alpha

   Plasmid

   #82719

   Purpose

   Expresses constitutively active human MKK6 and murine p38 alpha.

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Inserts

   MKK6 (MAP2K6 Human)
   p38alpha (Mapk14 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Ser207 to Glu, Thr211 to Glu

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-RT003-GPM

    Plasmid

    #163383

    Purpose

    To express sGFP module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    GFP-PDGFRtm-2A-mCherry (GPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC019 - LwCas13a from Leptotrichia wadei

    Plasmid

    #91909

    Purpose

    Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a and guide expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGLO-GFP-1UAG

    Plasmid

    #82500

    Purpose

    Expresses GFP with 1 UAG codon at the amino acid position 3

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescent Protein with 1 UAG codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3

    Promoter

    AraBAD

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDC216

    Plasmid

    #115791

    Purpose

    Expresses rat Syntaxin 1

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Syntaxin1 (Stx1a Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    C145S S193C C271S C272S

    Promoter

    T7

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T-1-3xFLAG

    Plasmid

    #129570

    Purpose

    Empty backbone for FLAG tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1159

    Plasmid

    #119250

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_Cas9d_Plasmid

    Plasmid

    #239445

    Purpose

    Expression plasmid codon-optimized for E. coli

    Depositor

    David Taylor

    Article

    Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

    Insert

    Cas9d
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28_ancCas9d_Plasmid

    Plasmid

    #239444

    Purpose

    Expression plasmid codon-optimized for E. coli

    Depositor

    David Taylor

    Article

    Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

    Insert

    ancCas9d
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSWAP_Entry_H2_beta_CM

    Plasmid

    #184870

    Purpose

    pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. PT7lacO-EF-Tu-His8

    Plasmid

    #224370

    Purpose

    Express EF-Tu protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    EF-Tu (tufB E. coli)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge