-
Plasmid#49998PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertG13 promoter + Firefly luciferase Red Thermostable
UseMycobacteria integrating vectorTagsExpressionBacterialMutationCodon optimized for Mycobacterium tuberculosis wi…PromoterG13Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJOE7706.1
Plasmid#135075PurposeA shuttle vector for heterologous HA production with Corynebacterium glutamicumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-acrAB-sfGFP
Plasmid#195516PurposeInducible expression vector for transcriptionally fused acrAB and sfGFP.DepositorInsertacrAB
UseSynthetic BiologyTagssfgfpExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule2-para-aminoPhe
Plasmid#85503PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor WT
Plasmid#12150DepositorInsertRhoA Biosensor (RHOA Human)
UseTags6xHisExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKD236-4b
Plasmid#90956PurposeExpresses ThsS constitutivelyDepositorInsertThsS
UseTagsExpressionBacterialMutationPromoterBba_J23104Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKP112 (pRS406-GFP-ADH1t)
Plasmid#106472PurposeVector for creating C-terminal GFP fusion proteins in yeast.DepositorTypeEmpty backboneUseTagsGFPExpressionBacterial and YeastMutationPromoterNoneAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG010 (DRT type 2)
Plasmid#157888PurposeExpresses the DRT type 2 defense systemDepositorInsertDRT type 2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(2A-9) pET29b
Plasmid#75145Purposereprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificityDepositorInsertreprogrammed sortase A variant
UseTags6x HisExpressionBacterialMutationS102C A104H E105D K138P K152I N160K K162H T164N K…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pALOD4
Plasmid#111026PurposeExpresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472ADepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472APromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUC01
Plasmid#177204Purposechromogenic reporter for gene knockout in StreptomycesDepositorInsertSaindC
UseSynthetic BiologyTagsNONEExpressionBacterialMutationNONEPromoterTGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 GOF
Plasmid#53200PurposeExpresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationR67S/D321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2-SNAP
Plasmid#208769PurposeTo express C2-SNAP in bacteriaDepositorInsertC2-SNAP (Mfge8 Mouse)
UseTagsSNAP-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-Broccoli
Plasmid#66788PurposeExpresses Broccoli in the F30 scaffold in bacterial cellsDepositorInsertBroccoli in the F30 scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSm-Nuclease
Plasmid#132431PurposeExpresses Serratia marcescens nuclease in E. coli using T7 promoterDepositorInsertSerratia marcescens nuclease (codon optimized)
UseTagsNoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV WT SPOP 28-359
Plasmid#194115PurposeWild type His-SUMO SPOP expression plasmidDepositorInsertSPOP (SPOP Human)
UseTags6His-SUMO-TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mu-IFNg-pCIneo
Plasmid#163517PurposeExpresses murine IFN gamma in mammalian cellsDepositorInsertmurine-IFN gamma (Ifng Mouse)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-ecfp
Plasmid#65030PurposeGmR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -