31,059 results
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Plasmid#175512PurposeFor protein expression of WT nsp1 CoV2DepositorInsertnsp1 CoV2
TagsGST tagExpressionBacterialPromotertac promoterAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5e-Rubicon RH
Plasmid#216706PurposeBacterial protein expression of Rubicon RH domainDepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(NC)-SH2
Plasmid#46499DepositorInsertSHP-2(NC) (PTPN11 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-222PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN4036
Plasmid#74698PurposeOutput plasmid: PPhlF-PAmtR-YFPDepositorInsertPPhlF-PBetI-YFP
ExpressionBacterialAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYTK004
Plasmid#65111PurposeEncodes ConL2 as a Type 1 part to be used in the Dueber YTK systemDepositorInsertConL2
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
TagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…Available SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET24b-BR1.4
Plasmid#199436Purposeexpresses BAmRed1.4 in bacterial cellsDepositorInsertBAMRed1.4
Tagshis tagExpressionBacterialPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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6972 pPROEX-HTb-SSA1
Plasmid#1231DepositorAvailable SinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET11b-HA2
Plasmid#165992PurposeInfluenza HA ministem with removable HisTagDepositorInsertHA2
TagsFoldon and HisTagExpressionBacterialPromoterT7Available SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT316
Plasmid#8608DepositorInsertbarstar
ExpressionBacterialAvailable SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSEVA421-Cas9tr
Plasmid#138709PurposePlasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenesDepositorInserttracrRNA-Cas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
Tags6xHIS-TwinStrep-SUMOExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST-2K
Plasmid#44564Purposefor ectopic constitutive expression of two mycobacterial proteins in one vectorDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB
Plasmid#30505DepositorAvailable SinceNov. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGRG37
Plasmid#16667DepositorInsertmTn7::Gateway cassette
ExpressionBacterialAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSR1037
Plasmid#208736PurposeCharacterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)DepositorInsertPSarA1
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-h-cGASCD(K427E/K428E)
Plasmid#127162Purposeexpression of truncated human cGAS proteinDepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab3b
Plasmid#203685PurposeExpresses N8C_Fab3bDepositorInsertN8C_Fab3b
ExpressionBacterialAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaSeph
Plasmid#67507PurposeExpress the tRNAMet scaffold with the sephadex aptamer tagDepositorInsertmodified tRNAMet with sephadex aptamer
ExpressionBacterialPromoterlppAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
O-TREM2-2
Plasmid#166545PurposescFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
poRibo-T2
Plasmid#69347PurposeRibo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutationDepositorInsertoRibo-T
ExpressionBacterialAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
TAF1
Plasmid#39117PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialPromoterpPH (Polyhedrin promoter)Available SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_PCNA_IRES_puro2b
Plasmid#21048DepositorInserthPCNA
TagsGFPExpressionBacterial and MammalianAvailable SinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PRKACB_Pkinase
Plasmid#110024PurposeProtein expression and purification of PRKACB_PkinaseDepositorInsertPRKACB_Pkinase (PRKACB Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialPromoterPtrc coreAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCH
Plasmid#164160PurposeIncQ BHR plasmidDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRECMYC-Ebu1
Plasmid#240713PurposepLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155DepositorInsertEbu1 RT, Eco1 ncRNA, MspRecT
ExpressionBacterialMutationWTAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRExpressionBacterialPromoterTetR-PT5-3Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRExpressionBacterialPromoterP3 constitutive promoter and native promoterAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab2b
Plasmid#203692PurposeExpresses N8C_Fab2bDepositorInsertN8C_Fab2b
ExpressionBacterialAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE5770
Plasmid#170777Purposeexpression of hybrid proteins in E. coliDepositorAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCPP3373
Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
ExpressionBacterialMutationSynonymous point mutation in the wobble position…Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-mCh-FYVE
Plasmid#192289PurposeExpresses GST- and mCherry-tagged HRS FYVE in e ColiDepositorInsertHRS FYVE domain
TagsGST-TEV-mCherryExpressionBacterialPromotertacAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFCiKm7
Plasmid#167521PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.DepositorInsertCinRAM-KmR
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIV296
Plasmid#204687PurposeAMA1 plasmid with nat1 resistance markerDepositorInsertnat1
UseFungal expressionExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG163
Plasmid#124365PurposeIPTG inducible system (gfpmut2 with Ppsank) for expression in B. subtilisDepositorAvailable SinceJuly 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L Hu IL-15-His tag
Plasmid#183256PurposeBacterial expression of human IL-15 with N-terminal His tagDepositorAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-dddAI
Plasmid#186559PurposeBroad host range regulated expression of the DddA immunity determinant (DddAI)DepositorInsertdddAI
ExpressionBacterialPromotertacAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL001
Plasmid#98917PurposeConstitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.DepositorInsertCaPR
ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pFVK5
Plasmid#167522PurposeBroad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.DepositorInsertVanRAM-mKelly2
UseSynthetic BiologyExpressionBacterialPromoterPVanCCAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CPDSalI
Plasmid#38252DepositorTypeEmpty backboneTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialPromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCas1+2
Plasmid#72676PurposeExpress Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.DepositorInsertCas1, Cas2
ExpressionBacterialAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialPromoteraraC and lppAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG166
Plasmid#124366PurposeGfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilisDepositorAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST HP1 beta
Plasmid#24075DepositorAvailable SinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only