32,656 results
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Plasmid#110142PurposeSuicide plasmid with repair template for knockout in P. putidaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only
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D. vulgaris sp2 CRISPR/pACYCDuet-1
Plasmid#81186PurposeExpresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coliDepositorInsertSynthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
UseCRISPRTagsNoneExpressionBacterialPromoterT7Available SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-N
Plasmid#30225DepositorInsertrhVitronectin with N terminus cleavage (VTN Human)
ExpressionBacterialMutationdeleted amino acids: 20*-62Available SinceMay 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pST44-AP-1 core
Plasmid#184809PurposeBacterial expression of clathrin adaptor AP-1 core complexDepositorInsertHs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
TagsGST tag and His6ExpressionBacterialMutationAP1S3: S148C; AP1B1: K359R, E476KPromoterT7Available SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-Pcym
Plasmid#225280PurposeMini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneExpressionBacterialPromoterPcym cumate (p-isopropylbenzoate)-inducible promo…Available SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAB076i3
Plasmid#79198PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW334i
Plasmid#237319Purposeexpresses A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1DepositorInsertA. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1
ExpressionBacterialAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-KOpstS
Plasmid#237503PurposeKnockout of pstSDepositorInsertphosphate ABC transporter substrate-binding protein PstS
ExpressionBacterialAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-URP112
Plasmid#237504PurposeUpregulation of mgdh with P112DepositorInsertmembrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
ExpressionBacterialAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
His-lsMSP1E3D1
Plasmid#240152PurposeExpression of the MSP 'His-lsMSP1E3D1'DepositorInsertHis-lsMSP1E3D1
TagsHis-tagExpressionBacterialPromoterT7 promoterAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVTRE3_smFP_Myc
Plasmid#201208PurposeAAV vector for TRE(TRE3G)-driven smFP_Myc (Myc-tagged spaghetti monster) expressionDepositorInsertsmFP _Myc (from addgene #59757)
UseAAVExpressionBacterial and MammalianPromoterTRE3GAvailable SinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His_TEV_MsbA
Plasmid#191476PurposeExpresses MsbA with a TEV protease cleavable N terminal His TagDepositorInsertMsbA
TagsHisExpressionBacterialPromoterT7Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM656
Plasmid#68887PurposeRhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_MYRF
Plasmid#64843PurposeA lentiviral vector for the expression of mouse MYRF under doxycyline control.DepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHuji
Plasmid#61555PurposeRed fluorescent protein based pH indicator in bacterial expression vectorDepositorInsertpHuji
Tags6xHisExpressionBacterialAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
AARS_C2_pNIC-Bio3
Plasmid#153047PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-mKelly2
Plasmid#119783PurposeExpresses mKelly2 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmKelly2
Tags6xHis tagExpressionBacterialPromoterT7Available SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSHDY
Plasmid#137661PurposeConjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 SARS-CoV 3CLpro
Plasmid#61692Purposeinducible expression of SARS 3CLpro proteaseDepositorInsertSARS 3CLpro protease
TagsGST and HISExpressionBacterialPromotertacAvailable SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUS200
Plasmid#17788DepositorInsertCytochrome P450cam
ExpressionBacterialAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBAC-BA-lacZ
Plasmid#13423DepositorInsertBA
UseCre/LoxExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTCF236 HLA-B*40:01
Plasmid#180463PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*40:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUWL201PW-creDE
Plasmid#112241PurposeStreptomyces/E.coli shuttle expression vector encoding creD and creEDepositorInsertscreD
creE
ExpressionBacterialAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
SB_concave
Plasmid#110102PurposepBXPHM3 with scaffold Sybody of the concave libraryDepositorInsertscaffold Sybody of the concave library
TagsHisExpressionBacterialPromoterPBADAvailable SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Rpb4/7
Plasmid#138484PurposeExpresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteriaDepositorTags6xHis and GSTExpressionBacterialPromotertacAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET Trx His6 LIC cloning vector (2Tc-T)
Plasmid#37239DepositorTypeEmpty backboneTags6xHis and Thioredoxin (Trx)ExpressionBacterialPromoterT7Available SinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbE8a-RFP
Plasmid#35268DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - C2 (UCSF)
Plasmid#169209PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
Tags6xHisExpressionBacterialPromoterT7Available SinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET.HaloTag.v7.AviTag
Plasmid#195486PurposeBacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.DepositorInsertHaloTag.v7.AviTag
Tags6xHIS and AviTagExpressionBacterialPromoterT7Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159548PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTS-HA-GFP11
Plasmid#177725PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA taggedDepositorInsertMTS-HA-GFP11
TagsGFP11 and HAExpressionBacterial and MammalianAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-mCherry
Plasmid#71737PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertmCherry
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)
Plasmid#159546PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)DepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_2
Plasmid#60574Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_2
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
Tags6xHis and MBPExpressionBacterialAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-KSRP
Plasmid#23019DepositorAvailable SinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFNR5
Plasmid#149483PurposeBroad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPSalTTCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7II-1N3Rtau
Plasmid#249552PurposeRecombinant protein synthesis of 1N3R isoform of human TauDepositorInsertTau (1N3R) (MAPT Human)
ExpressionBacterialAvailable SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pT7II-0N3Rtau
Plasmid#249553PurposeRecombinant protein synthesis of 0N3R isoform of human TauDepositorInsertTau (0N3R) (MAPT Human)
ExpressionBacterialAvailable SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X_MBP-LcgcN(C24S)-H6
Plasmid#245527PurposeMaltose binding protein N-terminally fused to His-tagged N-terminal LCGC14 PolB2split intein fragmentDepositorInsertN-terminal Fragment of the LCGC14 Split Intein with C24S Mutation
TagsHexahistidine Tag and Maltose Binding Protein (MB…ExpressionBacterialMutationC24S within the N-terminal fragment of the LCGC14…PromotertacAvailable SinceNov. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)
Plasmid#228661PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assayDepositorInsertZika virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
USUV strain Vienna 2001 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228658PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Usutu virus for biochemical assayDepositorInsertUsutu virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
POWV strain LB NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228654PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assayDepositorInsertPowassan virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialMutationAlanine mutant to stop self-cleaving ..VEREEA*AL.…Available SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228653PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assayDepositorInsertMVEV portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Fusion of West Nile NS2B-NS3 proteins
Plasmid#204796PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
ExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCarCBADE
Plasmid#117757PurposeCodon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised CarCBA, Protein CarD, Ferredoxin CarE
ExpressionBacterialPromoterPLac(UV5)Available SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-155
Plasmid#118569PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only